The
            <scp>C</scp>
            ‐terminal tail of tetraspanin proteins regulates their intracellular distribution in the parasite
            <scp>
              <i>T</i>
            </scp>
            <i>richomonas vaginalis</i>

Cóceres, Verónica M.; Alonso, Andrés M.; Nievas, Y. Romina; Midlej, Victor; Frontera, Lorena Soledad; Benchimol, Marlene; Johnson, Patricia J.; Miguel, Natalia de

doi:10.1111/cmi.12431

Cited by 38 publications

(52 citation statements)

References 47 publications

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“…We have previously noted that highly adherent strains generally aggregate in cell culture, in contrast to less‐adherent strains (such as G3; Coceres et al, ). Moreover, this observation has been correlated with the ability of the strain to adhere and be cytotoxic to host cells (Coceres et al, ; Lustig et al, ).…”

Section: Resultsmentioning

confidence: 96%

“…Despite being of critical importance to the survival of T. vaginalis , the mechanisms used to establish an infection and thrive within its host are poorly defined. Different T. vaginalis strains are highly variable in their adherence, aggregation, and cytolysis capacity (Coceres et al, ; Lustig, Ryan, Secor, & Johnson, ; Rojas et al, ), even when they have almost identical amino acid sequences (Leitsch et al, ; de Miguel et al, ; and our unpublished data). These differences in the pathogenic phenotype could suggest that the proteins mediating these interactions vary greatly, either in abundance or in type between strains (Lustig et al, ).…”

Section: Introductionmentioning

confidence: 80%

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Epigenetics regulates transcription and pathogenesis in the parasiteTrichomonas vaginalis

Pachano

Nievas

Lizarraga

et al. 2017

Cellular Microbiology

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Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract. Infections range from asymptomatic to highly inflammatory, depending on the host and the parasite strain. Different T. vaginalis strains vary greatly in their adherence and cytolytic capacities. These phenotypic differences might be attributed to differentially expressed genes as a consequence of extra-genetic variation, such as epigenetic modifications. In this study, we explored the role of histone acetylation in regulating gene transcription and pathogenesis in T. vaginalis. Here, we show that histone 3 lysine acetylation (H3KAc) is enriched in nucleosomes positioned around the transcription start site of active genes (BAP1 and BAP2) in a highly adherent parasite strain; compared with the low acetylation abundance in contrast to that observed in a less-adherent strain that expresses these genes at low levels. Additionally, exposition of less-adherent strain with a specific histone deacetylases inhibitor, trichostatin A, upregulated the transcription of BAP1 and BAP2 genes in concomitance with an increase in H3KAc abundance and chromatin accessibility around their transcription start sites. Moreover, we demonstrated that the binding of initiator binding protein, the transcription factor responsible for the initiation of transcription of ~75% of known T. vaginalis genes, depends on the histone acetylation state around the metazoan-like initiator to which initiator binding protein binds. Finally, we found that trichostatin A treatment increased parasite aggregation and adherence to host cells. Our data demonstrated for the first time that H3KAc is a permissive histone modification that functions to mediate both transcription and pathogenesis of the parasite T. vaginalis.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 80%

Epigenetics regulates transcription and pathogenesis in the parasiteTrichomonas vaginalis

Pachano

Nievas

Lizarraga

et al. 2017

Cellular Microbiology

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“…In general, tetraspanins are known to be involved in cell proliferation, adhesion, signalling and migration [56]. Recently, it was shown that the tetraspanin TvTSP8 of Trichomonas vaginalis is involved in parasite-parasite communication [57]. …”

Section: Discussionmentioning

confidence: 99%

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation

et al. 2016

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We here compared pathogenic (p) and non-pathogenic (np) isolates of Entamoeba histolytica to identify molecules involved in the ability of this parasite to induce amoebic liver abscess (ALA)-like lesions in two rodent models for the disease. We performed a comprehensive analysis of 12 clones (A1–A12) derived from a non-pathogenic isolate HM-1:IMSS-A and 12 clones (B1–B12) derived from a pathogenic isolate HM-1:IMSS-B. “Non-pathogenicity” included the induction of small and quickly resolved lesions while “pathogenicity” comprised larger abscess development that overstayed day 7 post infection. All A-clones were designated as non-pathogenic, whereas 4 out of 12 B-clones lost their ability to induce ALAs in gerbils. No correlation between ALA formation and cysteine peptidase (CP) activity, haemolytic activity, erythrophagocytosis, motility or cytopathic activity was found. To identify the molecular framework underlying different pathogenic phenotypes, three clones were selected for in-depth transcriptome analyses. Comparison of a non-pathogenic clone A1np with pathogenic clone B2p revealed 76 differentially expressed genes, whereas comparison of a non-pathogenic clone B8np with B2p revealed only 19 differentially expressed genes. Only six genes were found to be similarly regulated in the two non-pathogenic clones A1np and B8np in comparison with the pathogenic clone B2p. Based on these analyses, we chose 20 candidate genes and evaluated their roles in ALA formation using the respective gene-overexpressing transfectants. We conclude that different mechanisms lead to loss of pathogenicity. In total, we identified eight proteins, comprising a metallopeptidase, C2 domain proteins, alcohol dehydrogenases and hypothetical proteins, that affect the pathogenicity of E. histolytica.

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“…Genetic studies identified tetraspanins, proteins that are markers of exosomes 101. T. vaginalis exosomes are about 50-100 nm in diameter and contain RNA and a variety of proteins.…”

Section: Pathogenicity - Opening the "Black Box" Of Trichomonas Vaginmentioning

confidence: 99%

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

Menezes

Frasson

Tasca

2016

MIC

119

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Etiology: Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. Transmission: Trichomoniasis is transmitted by sexual intercourse and transmission via fomites is rare. Epidemiology, incidence and prevalence: The WHO estimates an incidence of 276 million new cases each year and prevalence of 187 million of infected individuals. However, the infection is not notifiable. Pathology/Symptomatology: The T. vaginalis infection results in a variety of clinical manifestations - in most cases the patients are asymptomatic, but some may develop signs typically associated to the disease. Importantly, the main issue concerning trichomoniasis is its relationship with serious health consequences such as cancer, adverse pregnancy outcomes, infertility, and HIV acquisition. Molecular mechanisms of infection: To achieve success in parasitism trichomonads develop a complex process against the host cells that includes dependent- and independent-contact mechanisms. This multifactorial pathogenesis includes molecules such as soluble factors, secreted proteinases, adhesins, lipophosphoglycan that culminate in cytoadherence and cytotoxicity against the host cells. Treatment and curability: The treatment with metronidazole or tinidazole is recommended; however, cure failures remain problematic due to noncompliance, reinfection and/or lack of treatment of sexual partners, inaccurate diagnosis, or drug resistance. Therefore, new therapeutic alternatives are urgently needed. Protection: Strategies for protection including sexual behavior, condom usage, and therapy have not contributed to the decrease on disease prevalence, pointing to the need for innovative approaches. Vaccine development has been hampered by the lack of long-lasting humoral immunity associated to the absence of good animal models.

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The C ‐terminal tail of tetraspanin proteins regulates their intracellular distribution in the parasite T richomonas vaginalis

Cited by 38 publications

References 47 publications

Epigenetics regulates transcription and pathogenesis in the parasiteTrichomonas vaginalis

Epigenetics regulates transcription and pathogenesis in the parasiteTrichomonas vaginalis

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation

Trichomoniasis – are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?

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