2023
DOI: 10.1002/em.22577
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The PIG‐A gene mutation assay in human biomonitoring and disease

Rachel Lawrence,
Kathryn Munn,
Hamsa Naser
et al.

Abstract: The blood cell phosphatidylinositol glycan class A (PIG‐A) gene mutation assay has been extensively researched in rodents for in vivo mutagenicity testing and is now being investigated in humans. The PIG‐A gene is involved in glycosyl phosphatidylinositol (GPI)‐anchor biosynthesis. A single mutation in this X‐linked gene can lead to loss of membrane‐bound GPI anchors, which can be enumerated via corresponding GPI‐anchored proteins (e.g., CD55) using flow cytometry. The studies published to date by different re… Show more

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Cited by 2 publications
(2 citation statements)
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References 62 publications
(147 reference statements)
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“…PIGA function cannot apparently be compensated for by any other member of the enzyme complex and thus loss of PIGA manifests in a deregulation of GPI-anchored proteins. GPI-anchored proteins and components of the GPI anchor biosynthesis pathway including PIGA are prognostic biomarkers in cancer ( 16 ). The ease of identifying PIGA mutant cells by flow cytometry ( 15 ) has led to the development of routine flow-based mutation detection assays for PNH and for mutagenicity in drug safety assessment ( 16 ).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…PIGA function cannot apparently be compensated for by any other member of the enzyme complex and thus loss of PIGA manifests in a deregulation of GPI-anchored proteins. GPI-anchored proteins and components of the GPI anchor biosynthesis pathway including PIGA are prognostic biomarkers in cancer ( 16 ). The ease of identifying PIGA mutant cells by flow cytometry ( 15 ) has led to the development of routine flow-based mutation detection assays for PNH and for mutagenicity in drug safety assessment ( 16 ).…”
Section: Discussionmentioning
confidence: 99%
“…GPI-anchored proteins and components of the GPI anchor biosynthesis pathway including PIGA are prognostic biomarkers in cancer ( 16 ). The ease of identifying PIGA mutant cells by flow cytometry ( 15 ) has led to the development of routine flow-based mutation detection assays for PNH and for mutagenicity in drug safety assessment ( 16 ).…”
Section: Discussionmentioning
confidence: 99%