The miR‐184‐3p promotes rice black‐streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)

Abstract

BACKGROUNDMicroRNAs (miRNAs) play a key role in various biological processes by influencing the translation of target messenger RNAs (mRNAs) through post‐transcriptional regulation. The miR‐184‐3p has been identified as an abundant conserved miRNA in insects. However, less is known about its functions in insect–plant virus interactions.RESULTSThe function of miR‐184‐3p in regulation of plant viral infection in insects was investigated using a rice black‐streaked dwarf virus (RBSDV) and Laodelphax striatellus (Fallén) interaction system. We found that the expression of miR‐184‐3p increased in L. striatellus after RBSDV infection. Injection of miR‐184‐3p mimics increased RBSDV accumulation, while treatment with miR‐184‐3p antagomirs inhibits the viral accumulation in L. striatellus. Ken, a zinc finger protein, was identified as a target of miR‐184‐3p. Knockdown of Ken increased the virus accumulation and promoted RBSDV transmission by L. striatellus.CONCLUSIONThis study demonstrates that RBSDV infection induces the expression of miR‐184‐3p in its insect vector L. striatellus. The miR‐184‐3p targets Ken to promote RBSDV accumulation and transmission. These findings provide a new insight into the function of the miRNAs in regulating plant viral infection in its insect vector. © 2023 Society of Chemical Industry.

MicroRNA750-3p Targets Processing of Precursor 7 to Suppress Rice Black-Streaked Dwarf Virus Propagation in Vector Laodelphax striatellus

“…For example, a small RNA derived from rice stripe virus (RSV) downregulates the miR263a of SBPH and then suppresses RSV infection [ 20 ]. Some researchers found that miR315-5p and miR184-3p could effectively modulate RBSDV infection in SBPH [ 21 , 22 ]. Li et al analyzed the conserved and novel miRNA profiles in RBSDV-infected SBPH and found miR750-3p was downregulated in RBSDV-infected SBPH [ 23 ].…”

Utilizing Star Polycation Nanocarrier for the Delivery of miR-184 Agomir and Its Impact on the Life History Traits of the English Grain Aphid, Sitobion avenae

“…In the pea aphid, Acyrthosiphon pisum (Aphididae, Hemiptera) , miR-184 negatively regulates the JNK (Jun N-terminal kinase) signaling pathway, which is involved in the immune response [ 18 ]. Moreover, miR-184 impacts the plant virus–host interactions by regulating the dynamics between rice black streak dwarf virus (RBSDV) and its insect vector, the planthopper Laodelphax striatellus (Delphacidae, Hemiptera), offering insights into pest-mediated virus transmission [ 19 ].…”