The <scp>P</scp>resence of <scp>SOD</scp> 1 and <scp>GSH</scp>‐<scp>P</scp>x in <scp>B</scp>ovine <scp>R</scp>etained and <scp>P</scp>roperly <scp>R</scp>eleased <scp>F</scp>oetal <scp>M</scp>embranes

Kankofer, Marta; Wawrzykowski, Jacek; Hoedemaker, Martina

doi:10.1111/rda.12152

Cited by 5 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For decorin detection, specific immune reaction was performed with 1 μg/ml sheep polyclonal anti-Decorin antibodies (RayBiotech, Norcross, Georgia) for 12 h at 4 °C. The visualization of immune reactions was prepared as previously described [ 13 ] with secondary polyclonal donkey antibody to sheep IgG conjugated with alkaline phosphatase at a concentration of 0.25 μg/ml. The obtained bands were compared with the decorin standard (RayBiotech, Norcross, Georgia).…”

Section: Methodsmentioning

confidence: 99%

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

2018

View full text Add to dashboard Cite

Decorin is a small leucine-rich proteoglycan which is involved in multiple biological functions mainly as a structural and signaling molecule. Due to its biological properties in connective tissue, decorin may participate in remodeling of ECM during attachment and detachment of placenta within the course of pregnancy and at parturition in cows. The aim of the study was to detect the presence of decorin protein in bovine placental tissues and to evaluate its profile during pregnancy and at parturition. Placental tissues from healthy pregnant cows (2–5 month) were collected in abattoir (n = 10), while parturient tissues were obtained during caesarian section at physiological term (n = 6). Maternal and fetal parts were separated manually and subjected to homogenization and to quantitative ELISA and verification by Western blotting with anti-decorin antibodies. ELISA test showed that the concentration of decorin during pregnancy was higher in the fetal part of placenta compared with the maternal part (p < 0.001). Similar pattern was noted regarding to maternal and fetal samples derived from parturient cows. Our preliminary results demonstrate that the concentration of decorin is gestation time-dependent in healthy bovine placenta. Possible confirmation of the involvement of decorin in early pregnancy attachment and detachment of the placenta during parturition requires further research.

show abstract

Section: Methodsmentioning

confidence: 99%

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

2018

View full text Add to dashboard Cite

show abstract

“…Interestingly, other authors considered the higher GPX activity in tissue of RP than in physiologically expelled placenta as part of the protective strengthening of the local antioxidant capacities (Kankofer et al. 2013 ). In our study, GPX inactivation in cows with RP by the increasing amount of reactive nitrogen species generated during the intensive APR might explain the disparity between GPX relative mass concentration and activity.…”

Section: Discussionmentioning

confidence: 99%

Profiling the alterations of serum proteome in dairy cows with retained placenta using high-throughput tandem mass tags quantitative approach

Beletić

Kuleš

Maslov

et al. 2023

Veterinary Quarterly

View full text Add to dashboard Cite

Background Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. Aim To investigate the difference in the serum proteome between the cows with RP and the physiologic puerperium (PP). Material & Methods Analysis of serum samples from nine cows with RP and six with PP using high-resolution liquid chromatography-tandem mass spectrometry approach. The proteins differing in the relative abundance between the PP and RP groups were classified using the Protein Analysis Through Evolutionary Relationship tool. For the pathway enrichment analysis, the REACTOME tool, with the human genome as the background, was employed. The criterion for significance was the false discovery rate corrected P-value less than 0.05. Results In total 651 proteins were identified with altered relative abundance of ten proteins. Among them, seven had higher, and three showed lower relative abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in lipoprotein metabolism, and the remaining ones associated with for instance redox homeostasis, post-translational modification, and scavenging. Finally, the validation of the proteomic results showed that haptoglobin and lipopolysaccharide-binding protein levels reliably differentiated between the RP and PP groups. Conclusion The pattern of serum proteome alterations in the cows with RP mirrored several interplaying mechanisms underlying the systematic response to the presence of RP, therefore representing a source to mine for predictive or prognostic biomarkers.

show abstract

“…For decorin detection, specific immune reaction was performed with 1 µg/ml sheep polyclonal anti‐decorin antibodies (RayBiotech) for 12 hr at 4°C. The visualization of immune reactions was prepared as previously described (Kankofer, Wawrzykowski, & Hoedemaker, 2013) with secondary polyclonal donkey antibody to sheep IgG conjugated with alkaline phosphatase at a concentration of 0.25 µg/ml. The obtained bands were compared with the decorin standard (RayBiotech).…”

Section: Methodsmentioning

confidence: 99%

Decorin concentrations in canine normal and neoplastic mammary gland tissues

Szczubiał

Kankofer

Wawrzykowski

et al. 2020

Reprod Domestic Animals

View full text Add to dashboard Cite

In the present study, the concentration of decorin in canine normal and neoplastic mammary gland tissues was examined to understand the potential role of decorin in development and progression of canine mammary tumours. The homogenates of 48 mammary gland tumours (10 benign and 38 malignant) and 10 samples of normal canine mammary gland tissue were used in the study. The presence and quantification of decorin was examined in the homogenates using Western blot and specific canine ELISA. Western blotting confirmed the presence of decorin both in the normal mammary gland tissues and in the mammary gland tumours. The concentration of decorin was significantly higher (p < .05) in the benign tumours and non-metastatic malignant tumours than in the normal mammary gland. The concentration of decorin was significantly lower (p < .05) in the malignant tumours with metastasis to regional lymph nodes compared with benign tumours and non-metastatic malignant tumours. No significant differences were found in the level of decorin between the benign and the non-metastatic malignant tumours. Both the histological type of malignant tumours and the histological grade did not significantly affect the concentration of decorin. These findings suggest that neoplastic transformation in the canine mammary gland leads to increase in the decorin protein synthesis. The reducing decorin concentration in canine malignant mammary tumours appears to facilitate the metastatic spread of these tumours.

show abstract

The Presence of SOD 1 and GSH‐Px in Bovine Retained and Properly Released Foetal Membranes

Cited by 5 publications

References 35 publications

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

Preliminary results of the placental decorin profile in bovine pregnancy and parturition

Profiling the alterations of serum proteome in dairy cows with retained placenta using high-throughput tandem mass tags quantitative approach

Decorin concentrations in canine normal and neoplastic mammary gland tissues

Contact Info

Product

Resources

About