The <scp>SKN</scp>‐1/Nrf2 transcription factor can protect against oxidative stress and increase lifespan in <i>C. elegans</i> by distinct mechanisms

Tullet, Jennifer M. A.; Green, James W.; Au, Catherine; Benedetto, Alexandre; Thompson, Maximillian A.; Clark, Emily; Gilliat, Ann F.; Young, Alex; Schmeißer, Kathrin; Gems, David

doi:10.1111/acel.12627

Cited by 142 publications

(101 citation statements)

References 20 publications

(31 reference statements)

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“…All three stress assays also clearly discriminated between strains with altered insulin/IGF‐1 signaling (IIS): a daf‐16(mgDf50) (FoxO) null mutant (stress sensitive), a daf‐16 overexpresser, and a daf‐2 insulin/IGF‐1 receptor reduction in function mutant (both stress resistant) (Figure e‐g). This is largely consistent with previous findings (Garsin et al, ; Henderson & Johnson, ; Lithgow, White, Melov, & Johnson, ; Tullet et al, , ), though the hypersensitivity of daf‐16 (mgDf50) to death from E. faecalis infection was not detected using previous methods (Garsin et al, ; Zou, Tu, Niu, Ji, & Zhang, ). Hence, this label‐free automated survival scoring method (henceforth referred to as LFASS) proved sensitive, robust, and accurate in a variety of assays, indicating its suitability for high‐throughput approaches.…”

Section: Resultssupporting

confidence: 91%

“…We therefore used the high temporal resolution capacity of LFASS to examine the effects of severe stress on C. elegans survival as they age. Specifically, we exposed adult worms to 7% t ‐BHP (by volume, i.e., 777 mM, as opposed to 5 or 7.5 mM, as used previously) (Tullet et al, , ) and 42°C (as opposed to 35°C as previously) (Labbadia & Morimoto, ), which in young adults both give a median survival time of only 1.5 hr (Figure a,b). We also studied how age affects resistance to accelerated E. faecalis OG1RF infection (death within 36 hr instead of 3 days) as a third severe stress paradigm.…”

Section: Resultsmentioning

confidence: 99%

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New label‐free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

Benedetto

Bambade

et al. 2019

Aging Cell

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Caenorhabditis elegans is an excellent model for high‐throughput experimental approaches but lacks an automated means to pinpoint time of death during survival assays over a short time frame, that is, easy to implement, highly scalable, robust, and versatile. Here, we describe an automated, label‐free, high‐throughput method using death‐associated fluorescence to monitor nematode population survival (dubbed LFASS for label‐free automated survival scoring), which we apply to severe stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity, and severe stress resistance, and its applicability to parasitic nematodes. The use of LFASS to assess the effects of aging on susceptibility to severe stress revealed an unexpected increase in stress resistance with advancing age, which was largely autophagy‐dependent. Correlation analysis further revealed that while severe thermal stress resistance positively correlates with lifespan, severe oxidative stress resistance does not. This supports the view that temperature‐sensitive protein‐handling processes more than redox homeostasis underpin aging in C. elegans. That the ages of peak resistance to infection, severe oxidative stress, heat shock, and milder stressors differ markedly suggests that stress resistance and health span do not show a simple correspondence in C. elegans.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

New label‐free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

Benedetto

Bambade

et al. 2019

Aging Cell

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“…Effects of L1‐12 on reactive oxygen species (ROS) level (a) and superoxide dismutase (SOD) activity (b) inside C. elegans . The nematodes were prepared with the same method as described in protocol . After 5 days, one part of worms was collected and washed with M9 buffer three times.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and antiageing properties of antioxidant pseudopeptides designed based on the bioisostere principle

Zhang

Wang

et al. 2019

Archiv der Pharmazie

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Nineteen antioxidant pseudopeptides were designed and synthesized. They were confirmed as mild antioxidants, in which L1-11 was the most active antioxidant with a cellular antioxidant activity (CAA) value of 5.65 ± 0.64 μmol QE/g, and L1-12 was the second most active one (5.58 ± 0.66 μmol QE/g). The existence of nonnatural amino acids in L1-12 increased its stability. Pretreatment with L1-12 dose-dependently extended the lifespan of Caenorhabditis elegans. L1-12 improved resistance against UVB irradiation, oxidative stress induced by paraquat, and thermal shock. It decreased the reactive oxygen species level and upregulated the superoxide dismutase activity inside C. elegans. This pseudopeptide sensitively enhanced the expressions of the Cat-1 and Nhr-8 genes to reduce oxidative damage, leading to an extension of the lifespan. All the evidence support that L1-12 may probably be a potential antiageing agent.

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“…This transcription factor is involved in diverse cellular function including the regulation cell death or apoptosis, resistance to oxidative stress and increased life span. Good references for the regulation of these transcription factors are available [54][55][56]. Hence, transcription factors that are critically involved in alleviation of oxidative stress and increased life span of C. elgans are activated by D. indusiata polysaccharides.…”

Section: Antioxidant Effectmentioning

confidence: 99%

The Chemistry, Pharmacology and Therapeutic Potential of the Edible Mushroom Dictyophora indusiata (Vent ex. Pers.) Fischer (Synn. Phallus indusiatus)

Habtemariam

2019

Biomedicines

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Dictyophora indusiata (Vent. Ex. Pers.) Fischer or Phallus indusiatus is an edible member of the higher mushroom phylum of Basidiomycetes. Known for its morphological elegance that gave it the names bridal veil fungus, veiled lady or queen of the mushrooms, it has numerous medicinal values that are beginning to be acknowledged through pharmacological efficacy studies. In an attempt to promote research on this valuable natural resource, the present communication aims to provide a comprehensive review of the chemistry, pharmacology and potential therapeutic applications of extracts and compounds isolated from D. indusiata. Of the bioactive compounds, the chemistry of the polysaccharides as major bioactive components primarily the β-(1→3)-D-glucan with side branches of β-(1→6)-glucosyl units are discussed, while small molecular weight compounds include terpenoids and alkaloids. Biochemical and cellular mechanisms of action from general antioxidant and anti-inflammatory to more specific signaling mechanisms are outlined along with potential applications in cancer and immunotherapy, neurodegenerative and chronic inflammatory diseases, etc. Further research areas and limitations of the current scientific data are also highlighted.Biomedicines 2019, 7, 98 2 of 21 have been reported [3]. The composition of carbohydrates can reach up to 47% of the dry weight of which the soluble polysaccharides are about 38%, while crude fibres and crude proteins contents are about 29% and 6% respectively [1]. In this communication, the emphasis is on the compositions of the fruiting body far beyond nutritional source with a focus on their pharmacologically active components and their therapeutic implications. In this connection, considerable attention has been given to the major components of the fungus, the polysaccharides, while small molecular weight drug-like molecules such as terpenoids and alkaloids have not been extensively studied. The chemistry and biological significance of all the bioactive compounds known from D. indusiata are herein scrutinised.Biomedicines 2020, 8, x FOR PEER REVIEW 2 of 21 have been reported [3]. The composition of carbohydrates can reach up to 47% of the dry weight of which the soluble polysaccharides are about 38%, while crude fibres and crude proteins contents are about 29% and 6% respectively [1]. In this communication, the emphasis is on the compositions of the fruiting body far beyond nutritional source with a focus on their pharmacologically active components and their therapeutic implications. In this connection, considerable attention has been given to the major components of the fungus, the polysaccharides, while small molecular weight drug-like molecules such as terpenoids and alkaloids have not been extensively studied. The chemistry and biological significance of all the bioactive compounds known from D. indusiata are herein scrutinised.

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The SKN‐1/Nrf2 transcription factor can protect against oxidative stress and increase lifespan in C. elegans by distinct mechanisms

Cited by 142 publications

References 20 publications

New label‐free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

New label‐free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

Synthesis and antiageing properties of antioxidant pseudopeptides designed based on the bioisostere principle

The Chemistry, Pharmacology and Therapeutic Potential of the Edible Mushroom Dictyophora indusiata (Vent ex. Pers.) Fischer (Synn. Phallus indusiatus)

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