The screening of miRNA/transcription factors-target gene regulatory networks in the aging process of hematopoietic stem cells

Abstract

Background hematopoietic stem cells (HSCs) failure is one of the key signs of biological aging. Objective Screening the key gene regulatory network in the aging process. Materials and methods Used GES32719 and GSE69408 to screen the difference genes in the Aging process, and enrichment analysis and protein-protein interaction analysis were carried out to obtain key transcription factor (TFs). The transcription factor is matched to the DNA damage map to obtain a target gene. Analysis of miRNA regulated or was regulated with transcription factors and target genes. Based on the above results, construct possible miRNA/TFs/target gene regulation networks. Results 222 different genes were obtained using the GES32719, found mitogen-activated protein kinase signaling pathway, microRNAs in cancer, and the hippo signaling pathway was significantly enrichment. Four TFS (MYC, FOS, ERG1, and JUN) were identified. Docked with DNA damage map, obtained three key target genes (PTEN, PFN1, and LMNA). 13 potential miRNAs based on TFS and target gene, where has-miR-139-5p and has-miR-543 were considered the most critical. Based on these data, three possible miRNA/TFs/target gene regulatory networks were constructed. Conclusion We constructed three possible regulatory models. This study provides a reference and basis for studying the possible mechanisms of HSCs aging.