The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

Shrivastava, Abhishek; Roland, Thibault; Berg, Howard C.

doi:10.1016/j.bpj.2016.07.043

Cited by 33 publications

(41 citation statements)

References 24 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The regulatory elements and biochemistry that have been discerned for M. xanthus, F. johnsoniae, M. mobile, and otherwise share no common homology outside their respective clusters. It is likely that gliding evolved independently in several bacterial classes (35), but each of these species exhibits characteristics consistent with tank tread locomotion mediated by membrane-spanning complexes (36)(37)(38).…”

mentioning

confidence: 99%

Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

et al. 2018

View full text Add to dashboard Cite

The degree to which surface motile bacteria explore their surroundings is influenced by aspects of their local environment. Accordingly, regulation of surface motility is controlled by numerous chemical, physical, and biological stimuli. Discernment of such regulation due to these multiple cues is a formidable challenge. Additionally inherent ambiguity and variability from the assays used to assess surface motility can be an obstacle to clear delineation of regulated surface motility behavior. Numerous studies have reported single environmental determinants of microbial motility and lifestyle behavior but the translation of these data to understand surface motility and bacterial colonization of human host or environmental surfaces is unclear. Here, we describe the current state of the field and our understanding of exogenous factors that influence bacterial surface motility.

show abstract

mentioning

confidence: 99%

Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

et al. 2018

View full text Add to dashboard Cite

show abstract

“…4Aand Movie S6-S12). The adhesin SprB of F. johnsoniae moves in a spiral fashion on the cell-surface(17). A non-motile bacterium on the surface of a C. gingivalis cell moves in a similar way(Fig.…”

mentioning

confidence: 88%

“…Bacteria that use motor-driven mobile cellsurface adhesins for motility are classified as gliding bacteria. Bacterial gliding requires a continuous expenditure of energy, and it only occurs when the bacteria are in contact with an external surface, with cells moving actively in a screw-like fashion [11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cargo Transport Shapes the Spatial Organization of a Microbial Community

Shrivastava

Patel

Tang

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

The human microbiome is an assemblage of diverse bacteria that interact with one another to form communities. Bacteria in a given community are arranged in a three-dimensional matrix with many degrees of freedom. Snapshots of the community display well-defined structures, but the steps required for their assembly are not understood. Here, we show that this construction is carried out with the help of gliding bacteria. Gliding is defined as the motion of cells over a solid or semi-solid surface without the necessity of growth or the aid of pili or flagella. Genomic analysis suggests that gliding bacteria are present in human microbial communities. We focus on Capnocytophaga gingivalis which is present in abundance in the human oral microbiome. Tracking of fluorescently-labeled single cells and of gas bubbles carried by fluid flow shows that swarms of C. gingivalis are layered, with cells in the upper layers moving more rapidly than those in the lower layers. Thus, cells also glide on top of one another. Cells of non-motile bacterial species attach to the surface of C. gingivalis and are propelled as cargo. The cargo cell moves along the length of a C. gingivalis cell, looping from one pole to the other. Multi-color fluorescent spectral imaging of cells of different live but non-motile bacterial species reveals their long-range transport in a polymicrobial community. A swarm of C. gingivalis transports some non-motile bacterial species more efficiently than others and helps shape the spatial organization of a polymicrobial community.SignificanceWe describe a situation in which bacteria typical of the human oral microbiome are organized spatially by gliding cells, species of Capnocytophaga, that move backwards and forwards over the substratum. The mobile adhesins that pull the cells over the substratum also attach to cells of non-motile bacterial species, which are carried up and down the motile cells as cargo. The synchronized transport of non-motile cargo bacteria helps shape a polymicrobial community.

show abstract

“…The motor in these bacteria powers rapid gliding motility across solid surfaces 8 using the PMF across the IM as the energy source 9–11 . The gliding motility motor generates a rotary motion at the cell surface 11 that results in a helical flow of surface adhesins 9,12,13 , possibly by driving a mobile track to which the adhesins are attached 14 .…”

Section: Mainmentioning

confidence: 99%

Structure of a proton-powered molecular motor that drives protein transport and gliding motility

Berks

James

Deme

et al. 2020

Preprint

View full text Add to dashboard Cite

16Ion-driven motors are rare in biology. The archetypes of the three classes identified to date are ATP 17 synthase, the bacterial flagellar motor, and a proton-driven motor that powers gliding motility and 18 protein secretion in Bacteroidetes bacteria. Whilst the molecular mechanism of ATP synthase is now 19 well understood, structural information is lacking for the other two classes of motor. Here we present 20 the structure of the Bacteroidetes gliding motility motor determined by cryo-electron microscopy. The 21 motor is an asymmetric inner membrane protein complex in which the single transmembrane helices 22Neither of these proteins has detectable sequence similarity to the components of the other classes 51 of ion-driven motors. GldL is composed of two predicted transmembrane helices (TMH) followed by a 52 long cytoplasmic tail 16 . GldM is mainly periplasmic and anchored to the IM by a N-terminal TMH 16 . A 53 recent structure of the periplasmic portion of GldM reveals that it contains four folded domains which 54 assemble as a homodimeric rod that is long enough (180Å) to span the periplasm 17 . The distal end of 55

show abstract

The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

Cited by 33 publications

References 24 publications

Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

Assessing Travel Conditions: Environmental and Host Influences on Bacterial Surface Motility

Cargo Transport Shapes the Spatial Organization of a Microbial Community

Structure of a proton-powered molecular motor that drives protein transport and gliding motility

Contact Info

Product

Resources

About