The abiotic stresses to which plants are exposed, especially in times of climate change, can result in the disruption of natural plant physiological processes. Sudden atmospheric phenomena may increase the risk of failure in protecting rare and extinction-threatened plant species by translocation. This study aimed to determine the effect of extreme ambient temperatures on the condition and physiological response of Salix lapponum plantlets used for their reintroduction into the natural habitat. Salix lapponum plants obtained by micropropagation methods at different stages of growth under laboratory conditions were subjected to a biological experiment. Plants were exposed for 12 h to temperature extremes (0 °C and 30 °C), after which the values of selected markers of the biochemical response were determined, such as photosynthetic pigments and anthocyanin content, guaiacol peroxidase and catalase activity, the presence of ROS and the RWC value. The study showed that plants at early growth stages were sensitive to low-temperature stress. In contrast, older ones showed a stronger response to high temperature, marked by an increased anthocyanin content and guaiacol peroxidase activity. It was also found that a short exposure to temperature extremes did not change the photosynthetic pigment content or catalase activity. The results of the study may be an important indication for the optimization of plant acclimatization methods in the process of their active protection by species translocation.