The SEC14 phospholipid transfer protein regulates pathogen-associated molecular pattern-triggered immunity in Nicotiana benthamiana

Kiba, Akinori; Nakano, Masahito; Ohnishi, Kouhei; Hikichi, Yasufumi

doi:10.1016/j.plaphy.2018.02.002

Cited by 13 publications

(8 citation statements)

References 35 publications

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“…Therefore, N. benthamiana leaves treated with 1 μM VdEIX3 were collected at 0, 3, 6, and 12 h post inoculation (hpi) for reverse transcription-quantitative PCR (RT-qPCR) analysis. The three selected MTI marker genes, PTI5, Acre31 (Kiba et al, 2018), CYP71D20 (Heese et al, 2007), and four pathogenesis-related genes PR1 (Dean et al, 2005), PR4 (Asai and Yoshioka, 2009), ERF1 (Zhang et al, 2017a(Zhang et al, , 2017b, and WRKY7 (Ishihama et al, 2011) were remarkably activated by the VdEIX3 treatment (Figure 3B, C). In particular, CYP71D20 and PR1 were upregulated by 8,000-and 10,000-fold, respectively.…”

Section: Vdeix3 Induces Immune Responses In N Benthamianamentioning

confidence: 91%

Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae

Yin

Wang

et al. 2021

JIPB

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Verticillium wilt diseases caused by the soil-borne fungus Verticillium dahliae result in devastating yield losses in many economically important crops annually. Here, we identified a novel ethyleneinducing xylanase (EIX)-like protein, VdEIX3, from V. dahliae, which exhibits immunity-inducing activity in Nicotiana benthamiana. In vitro-purified VdEIX3 can induce strong oxidative burst, activate the expression of defense-related genes, and increase resistance against oomycete and fungal pathogens in N. benthamiana. VdEIX3 orthologs of other Verticillium pathogens also induce cell death in N. benthamiana, which form a new type of EIX protein family that is distinct from the known EIX proteins. A leucine-rich repeat receptor-like protein, NbEIX2, regulates the perception of VdEIX3 in N. benthamiana. Our results demonstrate that VdEIX3 is a novel EIX-like protein that can be recognized by N. benthamiana NbEIX2, and also suggest that NbEIX2 is a promising receptor-like protein that is potentially applicable to transgenic breeding for improving resistance to Verticillium wilt diseases.

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Section: Vdeix3 Induces Immune Responses In N Benthamianamentioning

confidence: 91%

Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae

Yin

Wang

et al. 2021

JIPB

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“…Based on previous reports, the bacterial population of P. fluorescens 55 was adjusted to 10 7 ( Chakravarthy et al , 2010 ), P. syringae pv. tabaci 6605 to 10 4 ( Ito et al, 2014 a , 2014 b ), RsOE1-1 and Rs8107 to 10 8 ( Maimbo et al , 2010 ), and hrpY- deficient RsOE1-1 to 10 8 ( Kiba et al , 2018 ). Agrobacterium tumefaciens was cultured in YEB medium ( Maimbo et al , 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

Kiba

Nakano

Hosokawa

et al. 2020

Journal of Experimental Botany

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Phospholipid signaling plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here, we isolated two phospholipase C2 (PLC2) orthologs in the N. benthamiana genome, designated as PLC2-1 and 2-2. Both NbPLC2-1 and NbPLC2-2 were expressed in most tissues and were induced by infiltration with bacteria and flg22. NbPLC2-1 and NbPLC2-2 (NbPLC2s) double-silenced plants showed a moderately reduced growth phenotype. The induction of the hypersensitive response was not affected, but bacterial growth and the appearance of bacterial wilt were accelerated in NbPLC2s-silenced plants when they were challenged with a virulent strain of Ralstonia solanacearum that was compatible with N. benthamiana. NbPLC2s-silenced plants showed reduced expression levels of NbPR-4, a marker gene for jasmonic acid signaling, and decreased jasmonic acid and jasmonoyl-L-isoleucine contents after inoculation with R. solanacearum. The induction of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) marker genes was reduced in NbPLC2s-silenced plants after infiltration with R. solanacearum or Pseudomonas fluorescens. Accordingly, the resistance induced by flg22 was compromised in NbPLC2s-silenced plants. In addition, the expression of flg22-induced PTI marker genes, the oxidative burst, stomatal closure, and callose deposition were all reduced in the silenced plants. Thus, NbPLC2s might have important roles in pre- and post-invasive defenses, namely in the induction of PTI.

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“…RSSC strains first invade the intercellular spaces of plant roots (Araud‐Razou et al, 1998 ; Hikichi et al, 2017 ; Vasse et al, 1995 ). After invading the roots, the bacterial cells attach to the surface of host cells, where they avoid plant innate immune responses (Genin & Denny, 2012 ; Hikichi et al, 2017 ; Kiba et al, 2018 , 2020 ; Nakano et al, 2013 ) and grow vigorously to activate QS (Hikichi et al, 2017 ). The bacteria then invade xylem vessels, with the bacteria systemically spreading and multiplying throughout the xylem to activate QS (Genin & Denny, 2012 ; Hikichi et al, 2017 ; Vasse et al, 1995 ).…”

Section: Introductionmentioning

confidence: 99%

PhcQ mainly contributes to the regulation of quorum sensing‐dependent genes, in which PhcR is partially involved, in Ralstonia pseudosolanacearum strain OE1‐1

Takemura

Senuma

Hayashi

et al. 2021

Molecular Plant Pathology

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The gram‐negative plant‐pathogenic β‐proteobacterium Ralstonia pseudosolanacearum strain OE1‐1 produces methyl 3‐hydroxymyristate as a quorum sensing (QS) signal via the methyltransferase PhcB and senses the chemical through the sensor histidine kinase PhcS. This leads to functionalization of the LysR family transcriptional regulator PhcA, regulating QS‐dependent genes responsible for the QS‐dependent phenotypes including virulence. The phc operon consists of phcB, phcS, phcR, and phcQ, with the latter two encoding regulator proteins with a receiver domain and a histidine kinase domain and with a receiver domain, respectively. To elucidate the function of PhcR and PhcQ in the regulation of QS‐dependent genes, we generated phcR‐deletion and phcQ‐deletion mutants. Though the QS‐dependent phenotypes of the phcR‐deletion mutant were largely unchanged, deletion of phcQ led to a significant change in the QS‐dependent phenotypes. Transcriptome analysis coupled with quantitative reverse transcription‐PCR and RNA‐sequencing revealed that phcB, phcK, and phcA in the phcR‐deletion and phcQ‐deletion mutants were expressed at similar levels as in strain OE1‐1. Compared with strain OE1‐1, expression of 22.9% and 26.4% of positively and negatively QS‐dependent genes, respectively, was significantly altered in the phcR‐deletion mutant. However, expression of 96.8% and 66.9% of positively and negatively QS‐dependent genes, respectively, was significantly altered in the phcQ‐deletion mutant. Furthermore, a strong positive correlation of expression of these QS‐dependent genes was observed between the phcQ‐deletion and phcA‐deletion mutants. Our results indicate that PhcQ mainly contributes to the regulation of QS‐dependent genes, in which PhcR is partially involved.

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The SEC14 phospholipid transfer protein regulates pathogen-associated molecular pattern-triggered immunity in Nicotiana benthamiana

Cited by 13 publications

References 35 publications

Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae

Nicotiana benthamiana LRR‐RLP NbEIX2 mediates the perception of an EIX‐like protein from Verticillium dahliae

Phosphatidylinositol-phospholipase C2 regulates pattern-triggered immunity in Nicotiana benthamiana

PhcQ mainly contributes to the regulation of quorum sensing‐dependent genes, in which PhcR is partially involved, in Ralstonia pseudosolanacearum strain OE1‐1

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