The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy: a critical review

Marrelli, Mauro Toledo; Sallum, Maria Anice Mureb; Marinotti, Osvaldo

doi:10.1590/s0074-02762006000800002

Cited by 80 publications

(66 citation statements)

References 86 publications

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“…dunhami are available in GenBank, AF462378 and U92326. By comparing AF462378 with the consensus sequences generated in this study, we observed that they share 83-85% similarity, suggesting that they were generated from an individual of a distinct species (Marrelli et al 2006). When comparing the U92326 ITS2 sequence with that published by Lounibos et al (1998), we found that they are distinct but share the same GenBank accession number.…”

Section: Discussionmentioning

confidence: 64%

Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon

Calado

Foster

Bergo

et al. 2008

Mem. Inst. Oswaldo Cruz

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Nucleotide sequences of the internal transcribed spacer 2 (ITS2) rDNA and partial sequences of the cytochrome coxidase subunit I (COI) mtDNA and white gene nDNA were obtained from specimens of Anopheles nuneztovari A collected in Macapá (state of Amapá), Óbidos, Prainha and Almeirim (state of Pará), Itacoatiara and Parintins (state of Amazonas), Brazil, and compared with previously published sequences of A. nuneztovari s.l. Results of the Bayesian phylogenetic analyses performed using either COI or combined ITS2, COI and white gene sequences suggest that An. nuneztovari B/C is distinct from specimens obtained in the Amazonas/Solimões River basin. Anopheles goeldii, currently in synonymy with An. nuneztovari, was described from individuals collected in Belterra (= Fordlândia) in the Tapajós River, state of Pará, Southern Amazonas River. Morphological comparisons of the characteristics of the male genitalia indicated that An. nuneztovari A and An. goeldii are similar but distinct from An. nuneztovariB/C by the apex of the aedeagus. In considering the results of the phylogenetic analyses and morphological comparisons, An. goeldii is resurrected from synonymy with An. nuneztovari. Additionally, Anopheles dunhamiis reported for the first time in Parintins. This species can be distinguished from An. goeldiiby characters of the male genitalia and molecular data

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Section: Discussionmentioning

confidence: 64%

Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon

Calado

Foster

Bergo

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…and other closely related species and can compromise vector control strategies 12,13,19,28,29 ; in contrast, ITS2 is generally reliable. 27,30,31 All specimens collected were tested for the presence of Plasmodium using CytB-PCR but none were positive. However, the sample sizes were small, and as the overall average prevalence of Plasmodium in neotropical vectors is around 1%, this was not an unexpected result.…”

Section: Introductionmentioning

confidence: 99%

Molecular Taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and Malaria Epidemiology in Southern Amazonian Peru

Conn

Moreno²,

Saavedra³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either Anopheles (Nyssorhynchus) benarrochi B or An. (Nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as An. benarrochi B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian An. benarrochi B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA COI BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2+COI detected a single clade with very high support for all An. benarrochi B except one individual from Lupuna that was excluded. No samples were positive for Plasmodium by CytB-PCR.

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“…Several genetic markers have been studied in the previous studies, such as; ITS2 (Internal Transcribed Spacer), mitochondrial cytochrome c oxidase subunit 1 (COI) gene, and mitochocondrial NADH dehydrogenase subunit 4 (ND4) gene. ITS2 has been proven to be a good molecular marker because of its highly conserved region and species specific sequence; as a result, it is largely being used as a phylogenetic marker for mosquito's taxonomy (13,14).…”

Section: Phylogenetic Marker In Mosquitoesmentioning

confidence: 99%

Phylogenetic Analysis of Aedes aegypti Based on Mitochondrial ND4 Gene Sequences in Almadinah, Saudi Arabia

et al. 2016

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The second internal transcribed spacer of nuclear ribosomal DNA as a tool for Latin American anopheline taxonomy: a critical review

Cited by 80 publications

References 86 publications

Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon

Resurrection of Anopheles goeldii from synonymy with Anopheles nuneztovari (Diptera, Culicidae) and a new record for Anopheles dunhami in the Brazilian Amazon

Molecular Taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and Malaria Epidemiology in Southern Amazonian Peru

Phylogenetic Analysis of Aedes aegypti Based on Mitochondrial ND4 Gene Sequences in Almadinah, Saudi Arabia

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