The secondary structure of E. coli ribosomes and ribosomal RNA's: a spearophotometric approach

Araco, A.; Belli, M.; Giorgi, Colomba; Onori, G.

doi:10.1093/nar/2.3.373

Cited by 25 publications

(8 citation statements)

References 12 publications

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“…From phosphorus determinations it was found that the large and the small subunits of Artemia ribosomes contain 40.8 (± 1) pg of RNA per A260 unit. It is satisfactory that the same value has been found for particles with different ratios of RNA to protein content, because the contribution of the proteins to the absorbance at 260nm is negligible (Araco et al, 1975;Elson et al, 1979). The same value can therefore be used for the complete ribosomes also.…”

Section: Results and Discussion Relation Between Absorbance And Concesupporting

confidence: 68%

The molecular weight of Artemia ribosomes, as determined from their refractive-index increment and light-scattering intensity

Nieuwenhuysen

Voeght

Clauwaert

1981

Biochemical Journal

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Section: Results and Discussion Relation Between Absorbance And Concesupporting

confidence: 68%

The molecular weight of Artemia ribosomes, as determined from their refractive-index increment and light-scattering intensity

Nieuwenhuysen

Voeght

Clauwaert

1981

Biochemical Journal

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“…strand DNA as compared with double strand DNA and ribosomal RNA. It is possible, however, that the lack of detectable binding to RNA was due to the known secondary structure of the ribosomal species used ( 17,18).…”

Section: Discussionmentioning

confidence: 99%

Purification and general properties of the DNA-binding protein (P16) from rat liver mitochondria.

Pavco¹,

Tuyle²

1985

The Journal of Cell Biology

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The mitochondrial DNA-binding protein P16 was isolated from rat liver mitochondrial lysates by affinity chromatography on single strand DNA agarose and separated from DNA in the preparation by alkaline CsCI isopycnic gradients. The top fraction of the gradients contained a single polypeptide species (Mr ~ 15,200) based upon SDS PAGE. Digestion of single strand DNA-bound P16 with proteinase K produced a protease-insensitive, DNAbinding fragment (Mr ~ 6,000) that has been purified by essentially the same procedures used for intact P16. The partial amino acid compositions for P16 and the DNA-binding fragment were obtained by conventional methods. Analysis of subcellular fractions revealed that nearly all of the cellular P16 was located in the mitochondria and that only trace amounts of protein of comparable electrophoretic mobility could be isolated from the nuclear or cytoplasmic fractions. The labeling of P16 with [35S]methionine in primary rat hepatocyte cultures was inhibited by more than 90% by the cytoplasmic translation inhibitor cycloheximide, but unaffected by the mitochondrial-specific agent chloramphenicol. These results indicate that P16 is synthesized on cytoplasmic ribosomes and imported into the mitochondria. The addition of purified P16 to deproteinized mitochondrial DNA resulted in the complete protection of the labeled nascent strands of displacement loops against branch migrational loss during cleavage of parental DNA with SstI, thus providing strong evidence that P16 is the single entity required for this in vitro function. Incubation of P16 with single strand ~X174 DNA, double strand (RF) ~Xl 74 DNA, or Escherichia coil ribosomal RNA and subsequent analysis of the nucleic acid species for bound protein indicated a strong preference of P16 for single strand DNA and no detectable affinity for RNA or double strand DNA. Examination of P16-single strand ¢X174 DNA complexes by direct electron microscopy revealed thickened, irregular fibers characteristic of protein-associated single strand DNA.A previous study from our laboratory (1) identified P I6 (Mr ----16,000) as the single detectable polypeptide present in native mtDNA-protein complexes isolated from rat liver mitochondria. One function of the bound protein component was to stabilize the displacement loop (D-loop) structure by protecting the nascent strand of the D-loop from branch migration upon parental strand cleavage in vitro. In the accompanying paper (2), PI6 was found to bind exclusively to the displaced single strand of normal and expanded Dloops and to the single strand gap segment of molecules with the characteristics of/3-gapped circles. Thus, in addition to stabilization of the D-loop structure, P 16 probably functions at all stages of the asymmetrical replication cycle of mtDNA. Further evidence from that study indicated that there were an average of ~49 P16 molecules per mtDNA in the bound population composed predominantly of D-loop DNA.The work presented here describes the isolation of Pl 6 and a protease resistant, DNA...

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“…The situation with respect to 16 and 23 S procaryote rRNAs is of course less clear, but similar in outline. That is, a high degree of secondary (and possibly tertiary) structure is inferred from optical denaturation experiments (Cox et al 1973;Araco et al 1975). Metastable alternative structured forms appear to exist depending on the presence or absence of Mg 2+ (Morris, Dahlberg & Dahlberg, 1975;Nisbet & Slayter, 1975), and this is true also of the precursor molecules (Slack, Sartirana & Loening, 1975).…”

Section: (B) Ribosomal Rnamentioning

confidence: 98%

RNA structure

Kallenbach

Berman

1977

Quart. Rev. Biophys.

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This review is concerned primarily with the physical structure and changes in the structure of RNA molecules. It will be evident that we have not attempted comprehensive coverage of what amounts to a vast literature. We have tried to stay away from particular areas that have been recently reviewed elsewhere. Citations to and information from them are included, however, so that access to the literature is available. Much of what we treat in depth deals with the crystal structures and solution behaviour of model RNA compounds, including synthetic polymers and molecular fragments such as dinucleoside phosphates. Sequence data on natural RNA are cited, but not in detail. Similarly, apart from tRNA, natural RNAs the structural determinations of which are presently not so far advanced, are not dwelt upon. We have tried to present in detail the available structural data with scaled drawings that permit facile comparisons of molecular geometries.

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The secondary structure of E. coli ribosomes and ribosomal RNA's: a spearophotometric approach

Cited by 25 publications

References 12 publications

The molecular weight of Artemia ribosomes, as determined from their refractive-index increment and light-scattering intensity

The molecular weight of Artemia ribosomes, as determined from their refractive-index increment and light-scattering intensity

Purification and general properties of the DNA-binding protein (P16) from rat liver mitochondria.

RNA structure

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