The secreted ribonuclease <scp>T2</scp> protein <scp>FoRnt2</scp> contributes to <i>Fusarium oxysporum</i> virulence

Qian, Hengwei; Wang, Lulu; Wang, Baoshan; Liang, Wenxing

doi:10.1111/mpp.13237

Cited by 17 publications

(12 citation statements)

References 61 publications

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“…VdEG1 is another GH12 protein secreted by Verticillium dahliae and it plays an important role in virulence ( Gui et al, 2017 ). In addition, FoRnt2, a secreted ribonuclease T2 protein, contributes to the full virulence of F. oxysporum in tomato plants via its RNase activity ( Qian et al, 2022 ). Moreover, by targeting the host cell nucleus, the secreted Vd424Y protein from V. dahliae , a glycoside hydrolase family 11 protein, is required for full virulence ( Liu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The secreted FoAPY1 peptidase promotes Fusarium oxysporum invasion

Qian

Song²,

Wang³

et al. 2022

Front. Microbiol.

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The secretion of peptidases from several pathogens has been reported, but the biological function of these proteins in plant-pathogen interactions is poorly understood. Fusarium oxysporum, a soil-borne plant pathogenic fungus that causes Fusarium wilt in its host, can secrete proteins into host plant cells during the infection process to interfere with the host plant defense response and promote disease occurrence. In this study, we identified a peptidase, FoAPY1, that could be secreted from F. oxysporum depending on the N-terminal signal peptide of the protein. FoAPY1 belongs to the peptidase M28 family and exerts peptidase activity in vitro. Furthermore, the FoAYP1 gene knockout strain (∆FoAYP1) presented reduced virulence to tomato plants, but its mycelial growth and conidiation were unchanged. Moreover, FoAYP1 overexpression tomato seedlings exhibited enhanced susceptibility to F. oxysporum and Botrytis cinerea strains. These data demonstrated that FoAYP1 contributes to the virulence of F. oxysporum may through peptidase activity against host plant proteins.

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Section: Introductionmentioning

confidence: 99%

The secreted FoAPY1 peptidase promotes Fusarium oxysporum invasion

Qian

Song²,

Wang³

et al. 2022

Front. Microbiol.

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“…brongniartii, Cordyceps sp , Akanthomyces lecanii, Trichoderma sp., and Metarhizium sp , possess both BbRNT2 and BbTrv homologs. Yeast RNY1, plant fungal pathogens Fusarium oxysporum FoRnt2 and Ustilago maydis Nuc1 and Nuc2 were classified into the BbTrv clade, whereas a member of Colletotrichum viniferum was classified into the BbRNT2 clade (Figure C).…”

Section: Resultsmentioning

confidence: 99%

“…These effects may be due to their enzyme function in utilization of the extracellular RNA (presenting in maize apoplast) as a phosphate source at least . A secretory RNase T2, FoRnt2, of Fusarium oxysporum, is localized in both the cytoplasm and nucleus of plant cells and contributes to fungal virulence possibly through the degradation of host RNA …”

Section: Introductionmentioning

confidence: 99%

Two Secretory T2 RNases Act as Cytotoxic Factors Contributing to the Virulence of an Insect Fungal Pathogen

Yue

Deng

Wang

et al. 2023

J. Agric. Food Chem.

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RNase T2 members are secreted by several pathogens or parasites during infection, playing various roles in pathogen−host interaction. However, functions of those members in biocontrol microbes targeting their hosts are still unknown. Here, we report that an insect fungal pathogen, Beauveria bassiana, produces two secretory RNase T2 members that act as cytotoxic factors, which were examined by insect bioassays using the targeted gene(s) disruption and overexpression strains. Overexpression strains displayed dramatically increased virulence, which was concurrent with few fungal cells and hemocytes in hemocoel, suggesting a cytotoxicity of the overexpressed gene products. In vitro assays using yeast-expressed proteins verified the cytotoxicity of the two members against insect cells, to which the cytotoxic effect was dependent on their RNases enzyme activities and glycosylation modification. Moreover, the excessive humoral immune responses triggered by the two ribonucleases were examined. These results suggested prospects of these two T2 ribonucleases for improvement of biocontrol agents.

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“…We previously performed a secretome-wide analysis in Fol to provide new sight of infection-related proteins and found that most of them with an N-terminal SP were annotated as enzymes ( Li J. et al, 2020 ). To date, three secreted proteins are shown to contribute virulence, and two of them encode the ribonuclease and peptidase, respectively ( Li et al, 2022 ; Qian et al, 2022a , b ). In this study, we characterized one aspartic protease FolAsp from the Fol secretome.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the FolSvp1 is characterized to contribute virulence by translocating a key defense protein PR1 to dampen the host defense ( Li et al, 2022 ). FoRnt2, a secreted ribonuclease T2 protein, contributes to the full virulence of F. oxysporum in tomato plants depending on its RNase activity ( Qian et al, 2022a ). The FoAYP1 contributes to the virulence of Fol through peptidase activity on host protein ( Qian et al, 2022b ).…”

Section: Introductionmentioning

confidence: 99%

The secreted FolAsp aspartic protease facilitates the virulence of Fusarium oxysporum f. sp. lycopersici

et al. 2023

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Pathogens utilize secretory effectors to manipulate plant defense. Fusarium oxysporum f. sp. lycopersici (Fol) is the causal agent of Fusarium wilt disease in tomatoes. We previously identified 32 secreted effector candidates by LC-MS analysis. In this study, we functionally identified one of the secreted proteins, FolAsp, which belongs to the aspartic proteases (Asp) family. The FolAsp was upregulated with host root specifically induction. Its N-terminal 1–19 amino acids performed the secretion activity in the yeast system, which supported its secretion in Fol. Phenotypically, the growth and conidia production of the FolAsp deletion mutants were not changed; however, the mutants displayed significantly reduced virulence to the host tomato. Further study revealed the FolAsp was localized at the apoplast and inhibited INF1-induced cell death in planta. Meanwhile, FolAsp could inhibit flg22-mediated ROS burst. Furthermore, FolAsp displayed protease activity on host protein, and overexpression of FolAsp in Fol enhanced pathogen virulence. These results considerably extend our understanding of pathogens utilizing secreted protease to inhibit plant defense and promote its virulence, which provides potential applications for tomato improvement against disease as the new drug target.

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The secreted ribonuclease T2 protein FoRnt2 contributes to Fusarium oxysporum virulence

Cited by 17 publications

References 61 publications

The secreted FoAPY1 peptidase promotes Fusarium oxysporum invasion

The secreted FoAPY1 peptidase promotes Fusarium oxysporum invasion

Two Secretory T2 RNases Act as Cytotoxic Factors Contributing to the Virulence of an Insect Fungal Pathogen

The secreted FolAsp aspartic protease facilitates the virulence of Fusarium oxysporum f. sp. lycopersici

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