2009
DOI: 10.1002/pmic.200800692
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The secreted salivary proteome of the pea aphid Acyrthosiphon pisum characterised by mass spectrometry

Abstract: Nine proteins secreted in the saliva of the pea aphid Acyrthosiphon pisum were identified by a proteomics approach using GE-LC-MS/MS and LC-MS/MS, with reference to EST and genomic sequence data for A. pisum. Four proteins were identified by their sequences: a homolog of angiotensin-converting enzyme (an M2 metalloprotease), an M1 zinc-dependant metalloprotease, a glucose-methanol-choline (GMC)-oxidoreductase and a homolog to regucalcin (also known as senescence marker protein 30). The other five proteins are … Show more

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Cited by 224 publications
(296 citation statements)
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“…Seven nonsynonymous substitution sites were identified as evolving significantly faster than synonymous substitutions in the same genes, an indication that they are potentially under positive selection. Interestingly, the two glucose dehydrogenases identified in this study (ACYPI-000986 and ACYPI000288) also evolved at a faster rate relative to homologues in other insects, but in contrast the glucose dehydrogenase (ACYIPI000113; GMC oxidoreductase) identified in pea aphid saliva 28 has evolved at a significantly lower rate relative to other insect homologues. We found no evidence for positive selection among the predicted salivary glucose dehydrogenase genes (ACYPI000986 and ACYPI000288).…”
Section: Phylome Analysis Of Saliva-associated Genesmentioning
confidence: 82%
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“…Seven nonsynonymous substitution sites were identified as evolving significantly faster than synonymous substitutions in the same genes, an indication that they are potentially under positive selection. Interestingly, the two glucose dehydrogenases identified in this study (ACYPI-000986 and ACYPI000288) also evolved at a faster rate relative to homologues in other insects, but in contrast the glucose dehydrogenase (ACYIPI000113; GMC oxidoreductase) identified in pea aphid saliva 28 has evolved at a significantly lower rate relative to other insect homologues. We found no evidence for positive selection among the predicted salivary glucose dehydrogenase genes (ACYPI000986 and ACYPI000288).…”
Section: Phylome Analysis Of Saliva-associated Genesmentioning
confidence: 82%
“…The validity of this approach is supported by the fact that our resulting data set includes all but one (an unknown protein ACYPI008138) of the proteins identified by direct MS analysis of the saliva. 28 Further validation of our approach can be inferred from the high proportion of annotatable salivary-gland enriched transcript supported proteins that possess a secretion signal. This includes some of the proteases, oxidoreductases, and apolipophorin as well as some hypothetical proteins with homologues in other insect species.…”
Section: ' Discussionmentioning
confidence: 99%
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