The secretion and action of the digestive enzymes of the salivary glands of the blowfly, Calliphora

Bay, Christina M. Hansen

doi:10.1016/0022-1910(78)90111-7

Cited by 15 publications

(2 citation statements)

References 15 publications

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“…The blowfly Phormia regina feeds on rotting meat and fatty acids are an important component of its diet as they are required for reproduction (Stoffolano et al., ). During feeding, the flies secrete in their saliva a lipase that hydrolyses triglycerides, thus producing free fatty acids (Hansen Bay, ). These nutrients are not soluble in water and could not be ingested without the cooperation of a carrier.…”

Section: Multiple Functions Of Obps and Cspsmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are regarded as carriers of pheromones and odorants in insect chemoreception. These proteins are typically located in antennae, mouth organs and other chemosensory structures; however, members of both classes of proteins have been detected recently in other parts of the body and various functions have been proposed. The best studied of these non-sensory tasks is performed in pheromone glands, where OBPs and CSPs solubilise hydrophobic semiochemicals and assist their controlled release into the environment. In some cases the same proteins are expressed in antennae and pheromone glands, thus performing a dual role in receiving and broadcasting the same chemical message. Several reports have described OBPs and CSPs in reproductive organs. Some of these proteins are male specific and are transferred to females during mating. They likely carry semiochemicals with different proposed roles, from inhibiting other males from approaching mated females, to marking fertilized eggs, but further experimental evidence is still needed. Before being discovered in insects, the presence of binding proteins in pheromone glands and reproductive organs was widely reported in mammals, where vertebrate OBPs, structurally different from OBPs of insects and belonging to the lipocalin superfamily, are abundant in rodent urine, pig saliva and vaginal discharge of the hamster, as well as in the seminal fluid of rabbits. In at least four cases CSPs have been reported to promote development and regeneration: in embryo maturation in the honeybee, limb regeneration in the cockroach, ecdysis in larvae of fire ants and in promoting phase shift in locusts. Both OBPs and CSPs are also important in nutrition as solubilisers of lipids and other essential components of the diet. Particularly interesting is the affinity for carotenoids of CSPs abundantly secreted in the proboscis of moths and butterflies and the occurrence of the same (or very similar CSPs) in the eyes of the same insects. A role as a carrier of visual pigments for these proteins in insects parallels that of retinol-binding protein in vertebrates, a lipocalin structurally related to OBPs of vertebrates. Other functions of OBPs and CSPs include anti-inflammatory action in haematophagous insects, resistance to insecticides and eggshell formation. Such multiplicity of roles and the high success of both classes of proteins in being adapted to different situations is likely related to their stable scaffolding determining excellent stability to temperature, proteolysis and denaturing agents. The wide versatility of both OBPs and CSPs in nature has suggested several different uses for these proteins in biotechnological applications, from biosensors for odours to scavengers for pollutants and controlled releasers of chemicals in the environment.

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Section: Multiple Functions Of Obps and Cspsmentioning

confidence: 99%

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

et al. 2017

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“…Blowflies are the insects most commonly associated with crime scenes, and consequently several studies with a strong forensic focus have been conducted into the retrieval of human and mammalian DNA from blowflies (Table ). In both the juvenile (maggot) and adult stages, food will be degraded by enzymatic, bacterial, and physical activity, either prior to consumption as the insect breaks down solid tissue or dried food sources (Fraenkel, ; Graham‐Smith, ; Hansen Bay, , ; Hewitt, ; Kerlin & Hughes, ; Oschman & Berridge, ; Prince & Berridge, ; Waterhouse, ; Webber, ), or as it passes through the mid‐ and hindguts (Espinoza‐Fuentes & Terra, ; Hobson, ). Consequently, the DNA present within the food source will also degrade to some extent (Lund & Dissing, ; Pääbo, ; van Oorschot et al, ).…”

Section: Retrieval Of Extraneous Dna From Insectsmentioning

confidence: 99%

Insects as vectors of DNA in a forensic context

Durdle

2019

WIREs Forensic Science

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Insects have historically been used in criminal investigations to provide information in relation to postmortem intervals (PMIs) but the field of forensic entomology is expanding. It is now recognized that insects can act as vectors of human and mammalian DNA through the consumption of biological material, with extraneous DNA able to be extracted and genotyped from all stages of the life cycle, and insect feces and regurgitant (artifacts). To date, DNA recovered from insects have been used to inform investigations into neglect and homicide, link body parts, and to identify victims of crime. It may also potentially be used to identify assailants, confirm the food source of insects to determine their relevance in PMI calculations, determine if a crime has occurred, identify scenes of crimes, and link people to locations and other individuals. However, insects which have consumed biological material may also transfer DNA by traveling to new areas, or depositing it via their artifacts, either within crime scenes or laboratories, or at locations distant to the food source. This could contaminate forensic evidence, confound investigations, and/or falsely incriminate or exclude an individual. Therefore, it is important that there is increased awareness into both the utility of insects as vectors of forensically relevant DNA, and the potential for contamination. This article is categorized under: Forensic Biology > Forensic DNA Technologies Forensic Science in Action/Crime Scene Investigation > Crime Scene Examination Forensic Biology > Interpretation of Biological Evidence Forensic Biology > Body Fluid Identification

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The innervation of locust salivary glands

Baines¹,

Tyrer

1989

J. Comp. Physiol.

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The secretion and action of the digestive enzymes of the salivary glands of the blowfly, Calliphora

Cited by 15 publications

References 15 publications

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

Beyond chemoreception: diverse tasks of soluble olfactory proteins in insects

Insects as vectors of DNA in a forensic context

The innervation of locust salivary glands

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