The secretion antigen SA5K has a role in the adaptation of Mycobacterium bovis bacillus Calmette–Guérin to intracellular stress and hypoxia

Bottai, Daria; Batoni, Giovanna; Esin, Semih; Florio, Walter; Brancatisano, Franca Lisa; Favilli, Flavia; Maisetta, Giuseppantonio; Campa, Mario

doi:10.1016/j.micinf.2006.04.020

Cited by 3 publications

(2 citation statements)

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“…In addition to these clusters of antigens, a promising cell wall antigen is the secreted T-cell antigen TB8.4 (Rv1174c). This protein, detected in human macrophages and sputum of TB patients [ 215 ], affects growth of M. bovis BCG in human macrophages and mice [ 216 ], probably via its role in both resistance to intracellular stress and adaptation to hypoxia [ 217 ]. The Rv1174c knockout mutants are attenuated in vivo and Rv1174c-derived peptides were found to resuscitate one year old cultures [ 218 ].…”

Section: Resultsmentioning

confidence: 99%

Whole genome identification of Mycobacterium tuberculosisvaccine candidates by comprehensive data mining and bioinformatic analyses

et al. 2008

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Background: Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), infects ~8 million annually culminating in ~2 million deaths. Moreover, about one third of the population is latently infected, 10% of which develop disease during lifetime. Current approved prophylactic TB vaccines (BCG and derivatives thereof) are of variable efficiency in adult protection against pulmonary TB (0%-80%), and directed essentially against early phase infection.

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Section: Resultsmentioning

confidence: 99%

Whole genome identification of Mycobacterium tuberculosisvaccine candidates by comprehensive data mining and bioinformatic analyses

et al. 2008

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“…The impaired growth and virulence of the pmt mutant was also observed in the corresponding Mycobacterium abscessus mutant strain (Becker et al, 2017). Furthermore, secreted antigen SA5K (also called TB8.4 low molecular weight T-cell antigen), encoded by BCGsa5k, is associated with BCG intracellular adaption and survival (Bottai et al, 2006), while resuscitation-promoting factor RpfD, encoded by RN06_2923 in BCG, demonstrates strong immunogenicity in many mycobacterial infection models (Romano et al, 2012). Taken together, these findings suggested that the O-mannosylated proteins identified in the current study were potentially antigenic, and could be used to promote the immune responses induced by BCG.…”

Section: Discussionmentioning

confidence: 94%

Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980

Deng

Zhang

et al. 2020

Front. Microbiol.

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Bacterial glycoproteins have been investigated as vaccine candidates as well as diagnostic biomarkers. However, they are poorly understood in Mycobacterium bovis strain bacille Calmette-Guérin (BCG), a non-pathogenic model of Mycobacterium tuberculosis. To understand the roles of secreted O-mannosylated glycoproteins in BCG, we conducted a ConA lectin-affinity chromatography and mass spectra analysis to identify O-mannosylated proteins in BCG culture filtrate. Subsequent screening of antigens was performed using polyclonal antibodies obtained from a BCG-immunized mouse, with 15 endogenous O-mannosylated proteins eventually identified. Of these, BCG_0470 and BCG_0980 (PstS3) were revealed as the immunodominant antigens. To examine the protective effects of the antigens, recombinant antigens proteins were first expressed in Mycobacterium smegmatis and Escherichia coli, with the purified proteins then used to boost BCG primed-mice. Overall, the treated mice showed a greater delayed-type hypersensitivity response in vivo, as well as stronger Th1 responses, including higher level of IFN-7, TNF-α, and specific-IgG. Therefore, mannosylated proteins BCG_0470 and BCG_0980 effectively amplified the immune responses induced by BCG in mice. Together, our results suggest that the oligosaccharide chains containing mannose are the antigenic determinants of glycoproteins, providing key insight for future vaccine optimization and design.

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High Throughput Discovery of Novel Diagnostic Antigens for Mycobacterium bovis Using a Whole Genome Approach

Assal¹

2021

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The secretion antigen SA5K has a role in the adaptation of Mycobacterium bovis bacillus Calmette–Guérin to intracellular stress and hypoxia

Cited by 3 publications

References 31 publications

Whole genome identification of Mycobacterium tuberculosisvaccine candidates by comprehensive data mining and bioinformatic analyses

Whole genome identification of Mycobacterium tuberculosisvaccine candidates by comprehensive data mining and bioinformatic analyses

Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980

High Throughput Discovery of Novel Diagnostic Antigens for Mycobacterium bovis Using a Whole Genome Approach

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