The Secretome Deregulations in a Rat Model of Endotoxemic Shock

Blangy-Letheule, Angélique; Persello, Antoine; Michelland, Sylvie; Cunin, Valérie; Souab, F.; Aillerie, Virginie; Dhot, J.; Erraud, Angélique; Montnach, Jérôme; Sève, Michel; Bourgoin-Voillard, Sandrine; Rozec, Bertrand; Waard, Michel De; Lauzier, Benjamin

doi:10.1155/2021/6650464

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…Molecules with anti-inflammatory properties have been found in omega-3 fatty acids eicosapentaenoic acid, docosahexaenoic acid, and arachidonic acid ( 49 ). A study also showed that oxidative stress and lipid metabolism promote sepsis development ( 50 ). Furthermore, other pathways, including urea cycle metabolism, glutathione metabolism, and primary bile acid biosynthesis, were also affected in the MTS group.…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers and the mechanisms of multiple trauma complicated with sepsis using metabolomics

Feng

Dai

Liu

et al. 2022

Front. Public Health

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Sepsis after trauma increases the risk of mortality rate for patients in intensive care unit (ICUs). Currently, it is difficult to predict outcomes in individual patients with sepsis due to the complexity of causative pathogens and the lack of specific treatment. This study aimed to identify metabolomic biomarkers in patients with multiple trauma and those with multiple trauma accompanied with sepsis. Therefore, the metabolic profiles of healthy persons designated as normal controls (NC), multiple trauma patients (MT), and multiple trauma complicated with sepsis (MTS) (30 cases in each group) were analyzed with ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS)-based untargeted plasma metabolomics using collected plasma samples. The differential metabolites were enriched in amino acid metabolism, lipid metabolism, glycometabolism and nucleotide metabolism. Then, nine potential biomarkers, namely, acrylic acid, 5-amino-3-oxohexanoate, 3b-hydroxy-5-cholenoic acid, cytidine, succinic acid semialdehyde, PE [P-18:1(9Z)/16:1(9Z)], sphinganine, uracil, and uridine, were found to be correlated with clinical variables and validated using receiver operating characteristic (ROC) curves. Finally, the three potential biomarkers succinic acid semialdehyde, uracil and uridine were validated and can be applied in the clinical diagnosis of multiple traumas complicated with sepsis.

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Section: Discussionmentioning

confidence: 99%

Identification of biomarkers and the mechanisms of multiple trauma complicated with sepsis using metabolomics

Feng

Dai

Liu

et al. 2022

Front. Public Health

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“…The plasma secretome is easily accessible by a non-invasive approach and can be analyzed by non-targeted mass spectrometry [ 11 ]. Recently, a study identified, through the investigation of the secretome of a rat model in endotoxemic shock, deregulated proteins throughout the course of the pathology [ 101 ]. The analysis of the secretome of horses in sepsis could be a source of diagnostic, prognostic biomarkers, and therapeutic targets.…”

Section: Main Biomarkers Of Equine Sepsis: a Proof Of Concept For Hum...mentioning

confidence: 99%

Spontaneous Sepsis in Adult Horses: From Veterinary to Human Medicine Perspectives

Blangy-Letheule

Vergnaud

Dupas

et al. 2023

Cells

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Sepsis is a life-threatening disease defined as an organ dysfunction caused by a dysregulated host response to an infection. Early diagnosis and prognosis of sepsis are necessary for specific and timely treatment. However, no predictive biomarkers or therapeutic targets are available yet, mainly due to the lack of a pertinent model. A better understanding of the pathophysiological mechanisms associated with sepsis will allow for earlier and more appropriate management. For this purpose, experimental models of sepsis have been set up to decipher the progression and pathophysiology of human sepsis but also to identify new biomarkers or therapeutic targets. These experimental models, although imperfect, have mostly been performed on a murine model. However, due to the different pathophysiology of the species, the results obtained in these studies are difficult to transpose to humans. This underlines the importance of identifying pertinent situations to improve patient care. As humans, horses have the predisposition to develop sepsis spontaneously and may be a promising model for spontaneous sepsis. This review proposes to give first an overview of the different animal species used to model human sepsis, and, secondly, to focus on adult equine sepsis as a spontaneous model of sepsis and its potential implications for human and veterinary medicine.

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Endotoxin tolerance and low activation of TLR-4/NF-κB axis in monocytes of COVID-19 patients

et al. 2023

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Higher endotoxin in the circulation may indicate a compromised state of host immune response against coinfections in severe COVID-19 patients. We evaluated the inflammatory response of monocytes from COVID-19 patients after lipopolysaccharide (LPS) challenge. Whole blood samples of healthy controls, patients with mild COVID-19, and patients with severe COVID-19 were incubated with LPS for 2 h. Severe COVID-19 patients presented higher LPS and sCD14 levels in the plasma than healthy controls and mild COVID-19 patients. In non-stimulated in vitro condition, severe COVID-19 patients presented higher inflammatory cytokines and PGE-2 levels and CD14 + HLA-DR low monocytes frequency than controls. Moreover, severe COVID-19 patients presented higher NF-κB p65 phosphorylation in CD14 + HLA-DR low , as well as higher expression of TLR-4 and NF-κB p65 phosphorylation in CD14 + HLA-DR high compared to controls. The stimulation of LPS in whole blood of severe COVID-19 patients leads to lower cytokine production but higher PGE-2 levels compared to controls. Endotoxin challenge with both concentrations reduced the frequency of CD14 + HLA-DR low in severe COVID-19 patients, but the increases in TLR-4 expression and NF-κB p65 phosphorylation were more pronounced in both CD14 + monocytes of healthy controls and mild COVID-19 patients compared to severe COVID-19 group. We conclude that acute SARS-CoV-2 infection is associated with diminished endotoxin response in monocytes. Key messages Severe COVID-19 patients had higher levels of LPS and systemic IL-6 and TNF-α. Severe COVID-19 patients presented higher CD14+HLA-DRlow monocytes. Increased TLR-4/NF-κB axis was identified in monocytes of severe COVID-19. Blunted production of cytokines after whole blood LPS stimulation in severe COVID-19. Lower TLR-4/NF-κB activation in monocytes after LPS stimulation in severe COVID-19.

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The Secretome Deregulations in a Rat Model of Endotoxemic Shock

Cited by 4 publications

References 40 publications

Identification of biomarkers and the mechanisms of multiple trauma complicated with sepsis using metabolomics

Identification of biomarkers and the mechanisms of multiple trauma complicated with sepsis using metabolomics

Spontaneous Sepsis in Adult Horses: From Veterinary to Human Medicine Perspectives

Endotoxin tolerance and low activation of TLR-4/NF-κB axis in monocytes of COVID-19 patients

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