The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells

Abstract

The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.

Oxidative Stress Under Ambient and Physiological Oxygen Tension in Tissue Culture

“…Interestingly, mouse stem cells cultured at 3% O 2 showed less oxidative stress and lower aneuploidy, compared to the cells cultured at 21% O 2 [42]. Increased neuronal cell survival and differentiation of human neural progenitor cells is observed in human umbilical cord wharton jelly-derived mesenchymal stem cells (hWJ-MSCs) cultured at lower oxygen tension as compared to normoxia [43, 44]. On the contrary, culturing cells at 21% O 2 is known to induce chromosomal abnormality/aberration [41, 45, 46], aneuploidy [42], telomere shortening [47] and DNA damage [22] at genome levels in primary cells.…”

Insights into cell‐free therapeutic approach: Role of stem cell “soup‐ernatant”

“…One of the recent studies on adipose‐derived SC secretome has reported improved hepato‐regenerative activity after preconditioning of SCs with lipopolysaccharide, hence boosting the regenerative effect of SC secretome . The secretome from human Bone Marrow Stem Cells (h‐BMSCs) and h‐WJMSCs has shown survival and differentiation of a neuroblastoma cell line toward a neuronal phenotype . We have recently shown that secretome from four different human SC (BMSCs and dental SCs) populations induced the differentiation of IMR‐32 toward mature neuronal phenotype .…”

Canine mesenchymal stem cells are neurotrophic and angiogenic: An in vitro assessment of their paracrine activity

“…The human neuroblastoma cell line SH-SY5Y was used to assess neurotrophic activity of canine MSC CM, as has been performed previously with human MSC CM (Wright et al, 2010;Pires et al, 2014;Appendix: Supplementary material). This was due to a lack of available canine neuronal models, but also because we and other researchers have found similar responses to MSC secretomes in cell assays using MSCs and responder cells of the same and different species, i.e.…”