The Secretomes of Aspergillus japonicus and Aspergillus terreus Supplement the Rovabio® Enzyme Cocktail for the Degradation of Soybean Meal for Animal Feed

Abstract

One of the challenges of the 21st century will be to feed more than 10 billion people by 2050. In animal feed, one of the promising approaches is to use agriculture by-products such as soybean meal as it represents a rich source of proteins. However, soybean meal proteins are embedded in a complex plant cell wall matrix, mostly composed of pectic polysaccharides, which are recalcitrant to digestion for animals and can cause digestive disorders in poultry breeding. In this study, we explored fungal diversity to find enzymes acting on soybean meal components. An exploration of almost 50 fungal strains enabled the identification of two strains (Aspergillus terreus and Aspergillus japonicus), which improved the solubilization of soybean meal in terms of polysaccharides and proteins. The two Aspergilli strains identified in the frame of this study offer a promising solution to process industrial food coproducts into suitable animal feed solutions.

Quality improvement of soybean meal by yeast fermentation based on the degradation of anti‐nutritional factors and accumulation of beneficial metabolites

“…It has not, therefore, been applied on a large scale in feed industry. 14 Fermentation with lactic acid bacteria can form beneficial metabolites such as lactic acid and antimicrobial peptides, which can reduce the acid-binding capacity of feed and inhibit pathogenic bacteria. 15 However, a large amount of lactic acid produced by lactic acid bacteria fermentation leads to a single beneficial metabolite and inhibits the activity of protease.…”

“…However, aerobic fermentation requires forced ventilation, making investment more expensive, and it produces a large number of bitter peptides, affecting the palatability of the product. It has not, therefore, been applied on a large scale in feed industry 14 . Fermentation with lactic acid bacteria can form beneficial metabolites such as lactic acid and antimicrobial peptides, which can reduce the acid‐binding capacity of feed and inhibit pathogenic bacteria 15 .…”

Identification of Copper-Containing Oxidoreductases in the Secretomes of Three Colletotrichum Species with a Focus on Copper Radical Oxidases for the Biocatalytic Production of Fatty Aldehydes

“…The fungal kingdom represents a vast and untapped reservoir of biocatalysts (4) but to date, the enzymatic potential of filamentous fungi has mostly been studied in the frame of plant cell wall degradation (5)(6)(7). Fungal secretomics is a powerful approach to study the wide diversity of enzymes secreted and to formulate enzymatic cocktails with biotechnological relevance (8)(9)(10). Using suitable inducers, some fungal strains can secrete high amounts of oxidoreductases (11) including copper-containing enzymes.…”

Time-resolved secretome analysis of threeColletotrichumspecies identifies copper radical alcohol oxidases for the production of fatty aldehydes

“…The fungal kingdom represents a vast and untapped reservoir of biocatalysts (4) but to date, the enzymatic potential of filamentous fungi has mostly been studied in the frame of plant cell wall degradation (5)(6)(7). Fungal secretomics is a powerful approach to study the wide diversity of enzymes secreted and to formulate enzymatic cocktails with biotechnological relevance (8)(9)(10). Using suitable inducers, some fungal strains can secrete high amounts of oxidoreductases (11) (which was not certified by peer review) is the author/funder.…”