The secretory antibody system

Bazin, Hervé

doi:10.1007/978-94-011-6150-3_2

Cited by 16 publications

(2 citation statements)

References 159 publications

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“…The ACC were mostly localized in the crypts and the lower half of the villi, which is in accordance with earlier findings (17). Cells with specific intracellular staining were not seen in Peyer's patches, consistent with the reported absence of immunoglobulin-containing cells in the Peyer's patches (3,6). The lamina propria ACC seemed to be arranged in clusters and not randomly distributed in the specimens.…”

Section: Resultssupporting

confidence: 83%

“…The intestinal lamina propria contains vast numbers of immunoglobulin A (IgA)-producing plasma cells (3,6,8), and there is accumulating evidence that antibodies synthesized by these cells effect the major defense of the gut mucosal surface against pathogens (5,7,15,16,19). Although conventional immunohistological techniques can be employed to detect the specific antitoxin-containing plasma cells (ACC) in the lamina propria, knowledge is still very incomplete of the best conditions for stimulation of an effective ACC response in the small intestine.…”

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confidence: 99%

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Local cholera immunity in mice: intestinal antitoxin-containing cells and their correlation with protective immunity

Lange¹,

Hansson²,

Molin³

et al. 1979

Infect Immun

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Methods for light and electron microscopic identification and characterization of intestinal cholera antitoxin-containing cells (ACC) using peroxidase-labeled immunoreagent are described and used to study the ACC response in mice after immunizations with cholera toxin. Specific ACC appeared in significant numbers after two oral immunizations and increased six- to eightfold with two additional oral boosters, whereas further oral immunizations caused no additional stimulation. Intravenous immunizations had to be repeated seven times before ACC could be detected. After two oral immunizations, most of the ACC were found in the proximal part of the small intestine and no ACC were seen in the colon. This uneven distribution of ACC within the small intestine was eliminated after four oral immunizations, when ACC could also be detected in the colon. The ACC response after four oral immunizations demonstrated a peak at 4 days with far fewer cells present at 2 and 7 days. Electron microscopic studies showed that the ACC were mature plasma cells with the staining localized to the endoplasmic reticulum. Regression analysis of the relationship between the number of ACC and the magnitude of protective immunity against intestinal challenge with cholera toxin indicates a highly significant correlation (r = 0.91, P less than 0.001).

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Section: Resultssupporting

confidence: 83%