The Seedlesness Manifestation of Grape Varieties Under the Agro-Climatic Conditions of Anapa Ampelographic Collection

Ilnitskaya, Elena Tarasovna; Pyata, Elena Georgievna; Marmorshtein, Anna Aleksandrovna; Коваленко, Александр Григорьевич

doi:10.30679/2219-5335-2019-5-59-21-30

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“…Если рассматривать данные сорта в качестве роди-тельских форм для селекции бессемянных сортов, то следует отметить, что ягоды винограда генотипов Кишмиш запорожский, Памяти Смирнова и Шаян имеют достаточно крупные рудименты семян (Ilnitskaya et al, 2019a;Maistrenko et al, 2017 Рисунок. Профили локусов UDV305 и UDV737 сорта Памяти Смирнова при фрагментном анализе.…”

Section: таблица результаты днк-маркерного анализа сортов виноградаunclassified

“…В скобках указано значение с учётом корректировки относительно образца-контроля. (Remaily Seedless), характеризующийся практически полным отсутствием рудиментов семян в ягодах, что делает его наиболее привлекательным в качестве источника ценных признаков именно для селекции бессемянных сортов (Ilnitskaya et al, 2019a;Pool et al, 1981).…”

Section: таблица результаты днк-маркерного анализа сортов виноградаunclassified

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DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

Ильницкая

Makarkina

Токмаков

2020

Biotehnologiâ i selekciâ rastenij

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Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

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Section: таблица результаты днк-маркерного анализа сортов виноградаunclassified

DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

Ильницкая

Makarkina

Токмаков

2020

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

show abstract

The Seedlesness Manifestation of Grape Varieties Under the Agro-Climatic Conditions of Anapa Ampelographic Collection

Cited by 1 publication

References 0 publications

DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

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