The segmentation gene Krüppel of Drosophila melanogaster has homeotic properties.

Harbecke, Ruth; Janning, Wilfried

doi:10.1101/gad.3.1.114

Cited by 46 publications

(23 citation statements)

References 43 publications

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“…3B), which is thought to represent rudimental tissues of the small intestine intermingled with rudimental Malpighian tubules. These results demonstrate that the (Harbecke and Janning, 1989;Liu and Jack, 1992;Redemann et al, 1988;Skaer, 1993). In Kr embryos, drm was found to be expressed in the posterior-most region of the midgut (Fig.…”

Section: Development Of the Small Intestine In Mutations That Affect supporting

confidence: 51%

The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

Uddin¹,

Yano²,

Murakami³

2011

Int. J. Dev. Biol.

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An odd family gene drumstick (drm) encodes a zinc finger protein, and is necessary for the development of the small intestine, an anterior domain of the ectodermal hindgut of Drosophila melanogaster. However, mechanisms that specify the small intestine, as well as gene regulatory pathways leading to transcriptional activation of drm, are still unclear. We found that drm is expressed in two different tissues abutting the anterior end of the hindgut primordium, that is, the posterior-most region of the midgut (endoderm) and basal portion of the Malpighian tubules. A small intestine marker gene, unpaired (upd), begins to be expressed at the anterior-most region of the hindgut primordium that abuts the basal portion of Malpighian tubules, and the upd-positive region expands, resulting in a short tube during stages 11-13. The small intestine develops in both of the mutant embryos, serpent (srp) and Krüppel (Kr), that lack the drm-positive midgut or Malpighian tubules, respectively, while it fails to develop in the Kr srp double-mutant embryos that lack both of the drm-positive tissues. These results demonstrate that drm expressed in the abutting tissues cell-non-autonomously induces development of the small intestine in the hindgut primordium, probably by deploying some extracellular signaling factor. drm expression in the posterior gut region disappears and the small intestine fails to form in tailless (tll) mutant embryos, whereas over-expression of tll causes expansion of drm expression throughout the midgut, inducing a longer small intestine. These results indicate that drm is activated under the control of tll and triggers development of the small intestine cell-non-autonomously through some extracellular signaling.

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Section: Development Of the Small Intestine In Mutations That Affect supporting

confidence: 51%

The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

Uddin¹,

Yano²,

Murakami³

2011

Int. J. Dev. Biol.

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“…2 A and B). The phenotype of Kr mutants has been described as the complete loss of renal tubule cells (15,16) and of ct mutants as the failure of specified cells to branch out from the hindgut (12,14,17). Using the transport of urates as a diagnostic feature for differentiated tubule cell function, we show that it is only when the functions of both Kr and ct are removed that cell specification fails completely (Figs.…”

Section: Resultsmentioning

confidence: 99%

Genetic regulation of patterned tubular branching in Drosophila

Hatton-Ellis

Ainsworth

Sushama

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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A common theme in organogenesis is the branching of epithelial tubes, for example in the lung, liver, or kidney. The later morphogenesis of these branched epithelia dictates the final form and function of the mature tissue. Epithelial branching requires the specification of branch cells, the eversion process itself, and, frequently, patterned morphogenesis to produce branches of specific shape and orientation. Using the branching of renal tubule primordia from the hindgut in Drosophila, we show that these aspects are coordinately regulated. Cell specification depends on Wnt signaling along the tubular gut and results in the spatially restricted coexpression of two transcription factors, Krü ppel and Cut, in the hindgut, whose activity drives cells toward renal tubule fate. Significantly, these transcription factors also confer the competence to respond to a second signal; TGF-␤ induces branching to form the four renal tubule buds. Differential activation of the TGF-␤ pathway also patterns the tubules, resulting in the asymmetry in size and positioning that is characteristic of the two tubule pairs. High levels of TGF-␤ promote the expression of Dorsocross1-3 and anterior tubule growth, whereas low levels allow the expression of the transcriptional repressor, Brinker, and thus promote posterior tubule identity. We show that patterning of the tubule primordium into two distinct pairs is critical for the eversion of tubule branches, as well as for their asymmetric morphogenesis.branching morphogenesis ͉ patterning ͉ renal tubule ͉ TGF-␤ signaling ͉ Wnt

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“…However, it is clear that Kr alone is not su¤cient to dictate complete tubule cell di¡erentiation. In the absence of Kr, it is tubule eversion that fails and in mutants where Kr expression is reduced, but not lost, the tubules evert but their morphogenesis later in embryogenesis is defective (Harbecke & Janning 1989;Liu & Jack 1992).…”

Section: The Allocation Of Malpighian Tubule Cells and The Formation mentioning

confidence: 99%

“…This is followed by the changes in cell shape that mark the onset of tubule eversion. It has been suggested that the expression of Kr alone is su¤cient to propel cells into Malpighian tubule di¡erentiation; KrÏppel acts to switch potential hindgut cells into the tubule cell fate (Harbecke & Janning 1989). Indeed, in embryos mutant for Kr no tubules bud out from the hindgut (Gloor 1950).…”

Section: The Allocation Of Malpighian Tubule Cells and The Formation mentioning

confidence: 99%

Coordinating cell fate and morphogenesis inDrosophilarenal tubules

Ainsworth

Wan

Skaer

2000

Phil. Trans. R. Soc. Lond. B

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Using the renal tubules of Drosophila as an example, we explore how cell speci¢cation leads to the morphogenetic movements that underlie the generation of tissue architecture. Taking two stages of development, we show ¢rst that the tubule cells are allocated by signalling between the endodermal and ectodermal compartments of the posterior gut. Activation of the Wnt pathway patterns the ectodermal anlage, resulting in the expression of tubule genes in a subset of cells and their eversion from the hindgut to form the tubule primordia. We argue that early gene expression directs these morphogenetic movements but not the complete programme of tubule di¡erentiation. In the second example we show that the allocation of the mitogenic tip cell lineage in each tubule is required not only for the normal pattern of cell division but also for the stereotyped three-dimensional arrangement of the mature tubules. Analysis of mutants in which the tip cell lineage is misspeci¢ed reveals that both daughters of the tip cell progenitor are required for the tubules to navigate through the body cavity, so that the distal tips locate in their characteristic positions. We show that the regulator of Rac, Myoblast city, is essential for this second morphogenetic process.

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The segmentation gene Krüppel of Drosophila melanogaster has homeotic properties.

Cited by 46 publications

References 43 publications

The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

The drumstick gene acts cell-non-autonomously and triggers specification of the small intestine in the Drosophila hindgut

Genetic regulation of patterned tubular branching in Drosophila

Coordinating cell fate and morphogenesis inDrosophilarenal tubules

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