The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat

Leesukon, Panithi; Wirathorn, Wilaiwan; Chuchue, Tatsanee; Charoenlap, Nisanart; Mongkolsuk, Skorn

doi:10.1007/s00203-013-0913-7

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…To determine whether over-production of another unrelated MFS transporter increases mexX expression and reduces antibiotic susceptibility, PAO1 harboring pTetA(C) for overexpression of tetA(C) (Leesukon et al, 2013) was used in the experiments. The tetracycline resistance gene, tetA(C), encodes an MFS efflux pump containing 12 transmembrane domains that transport tetracycline across the inner membrane (Schnappinger and Hillen, 1996).…”

Section: Envelope Proteomics Analysis Of In Pao1/pmfs1 and Pao1/pmfs2mentioning

confidence: 99%

Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa

et al. 2021

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Pseudomonas aeruginosa, a well-known cause of nosocomial infection, is frequently antibiotic resistant and this complicates treatment. Links between oxidative stress responses inducing antibiotic resistance through over-production of RND-type efflux pumps have been reported in P. aeruginosa, but this has not previously been associated with MFS-type efflux pumps. Two MFS efflux pumps encoded by mfs1 and mfs2 were selected for study because they were found to be sodium hypochlorite (NaOCl) inducible. Antibiotic susceptibility testing was used to define the importance of these MFS pumps in antibiotic resistance and proteomics was used to characterize the resistance mechanisms involved. The results revealed that mfs1 is NaOCl inducible whereas mfs2 is NaOCl, N-Ethylmaleimide and t-butyl hydroperoxide inducible. Deletion of mfs1 or mfs2 did not affect antibiotic or paraquat susceptibility. However, over-production of Mfs1 and Mfs2 reduced susceptibility to aminoglycosides, quinolones, and paraquat. Proteomics, gene expression analysis and targeted mutagenesis showed that over-production of the MexXY RND-type efflux pump in a manner dependent upon armZ, but not amgRS, is the cause of reduced antibiotic susceptibility upon over-production of Mfs1 and Mfs2. mexXY operon expression analysis in strains carrying various lengths of mfs1 and mfs2 revealed that at least three transmembrane domains are necessary for mexXY over-expression and decreased antibiotic susceptibility. Over-expression of the MFS-type efflux pump gene tetA(C) did not give the same effect. Changes in paraquat susceptibility were independent of mexXY and armZ suggesting that it is a substrate of Mfs1 and Mfs2. Altogether, this is the first evidence of cascade effects where the over-production of an MFS pump causes over-production of an RND pump, in this case MexXY via increased armZ expression.

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Section: Envelope Proteomics Analysis Of In Pao1/pmfs1 and Pao1/pmfs2mentioning

confidence: 99%

Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa

et al. 2021

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Effects of full-scale advanced water treatment on antibiotic resistance genes in the Yangtze Delta area in China

Zhang

Lin

2016

FEMS Microbiology Ecology

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As emerging microbial contaminants, antibiotic resistance genes (ARGs) are widespread in the aquatic environment, including source water, which might enter water supply systems and endanger public health by enhancing the resistance of opportunistic pathogens to some antibiotics. In the present study, we investigated how water treatments affect the levels of ARGs in a full-scale drinking water treatment plant for one year using real-time PCR. The 16s rRNA gene and eleven ARG families, including tetA, tetG, aacC1, strA, ermB, cmlA5, vanA, dfrA1, sulII, blaTEM-1 and blaoxa-1, in source water and the outlet of each treatment and tap water were monitored. The results showed that nine ARG families were detected at relatively high levels, for example, the sulII gene was detected at ∼10(4) copies mL(-1) compared with 10(5) copies mL(-1) in finished water and tap water in July, whose relative concentrations were consistently high. Treatments for the reduction of the absolute concentrations of ARGs included sand filtration, coagulation/sedimentation and two-stage O3-BAC filtration, while distribution could increase ARGs an average of 0.50 log.

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The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat

Cited by 2 publications

References 15 publications

Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa

Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa

Effects of full-scale advanced water treatment on antibiotic resistance genes in the Yangtze Delta area in China

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