The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells

Ha, Do Thi; Trung, Trân Nam; Phượng, Trần Thị; Yim, Nam–Hui; Chen, Quan Cheng; Bae, KiHwan

doi:10.1016/j.bmcl.2010.08.054

Cited by 48 publications

(25 citation statements)

References 54 publications

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“…Moreover, activation of AMPK was previously associated with the inhibition of adipocyte differentiation [70] or increased glucose uptake. Indeed, our data are in an agreement with recent reports by Ha et al [71,72] demonstrating an increase of glucose uptake and inhibition of adipocyte differentiation through the activation of AMPK in 3T3-L1 cells.…”

Section: Discussionsupporting

confidence: 94%

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Thyagarajan‐Sahu

Lane

Slíva

2011

BMC Complement Altern Med

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BackgroundObesity is a health hazard which is closely associated with various complications including insulin resistance, hypertension, dyslipidemia, atherosclerosis, type 2 diabetes and cancer. In spite of numerous preclinical and clinical interventions, the prevalence of obesity and its related disorders are on the rise demanding an urgent need for exploring novel therapeutic agents that can regulate adipogenesis. In the present study, we evaluated whether a dietary supplement ReishiMax (RM), containing triterpenes and polysaccharides extracted from medicinal mushroom Ganoderma lucidum, affects adipocyte differentiation and glucose uptake in 3T3-L1 cells.Methods3T3-L1 pre-adipocytes were differentiated into adipocytes and treated with RM (0-300 μg/ml). Adipocyte differentiation/lipid uptake was evaluated by oil red O staining and triglyceride and glycerol concentrations were determined. Gene expression was evaluated by semi-quantitative RT-PCR and Western blot analysis. Glucose uptake was determined with [3H]-glucose.ResultsRM inhibited adipocyte differentiation through the suppresion of expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ (PPAR-γ), sterol regulatory element binding element protein-1c (SREBP-1c) and CCAAT/enhancer binding protein-α (C/EBP-α). RM also suppressed expression of enzymes and proteins responsible for lipid synthesis, transport and storage: fatty acid synthase (FAS), acyl-CoA synthetase-1 (ACS1), fatty acid binding protein-4 (FABP4), fatty acid transport protein-1 (FATP1) and perilipin. RM induced AMP-activated protein kinase (AMPK) and increased glucose uptake by adipocytes.ConclusionOur study suggests that RM can control adipocyte differentiation and glucose uptake. The health benefits of ReishiMax warrant further clinical studies.

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Section: Discussionsupporting

confidence: 94%

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Thyagarajan‐Sahu

Lane

Slíva

2011

BMC Complement Altern Med

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“…Previous studies reported that AMPK activators such as 5‐Aminoimidazole‐4‐carboxamide ribonucleotide (AICAR), A‐769662, RSVA314 and RSVA405 inhibit adipogenesis and decrease the levels of major genes involved in adipogenesis, including PPAR‐γ, C/EPBα and FAS. In addition, AICAR is effective to restore metabolic alterations in mice in which obesity was induced by feeding of high‐fat diets . These findings indicate that activation of AMPK is sufficient to terminate adipogenesis in pre‐adipocytes.…”

Section: Discussionmentioning

confidence: 85%

Euphorbiasteroid, a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3‐L1 cells via activation of AMP‐activated protein kinase

Park

Han

et al. 2015

Cell Biochemistry & Function

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The purpose of this study is to investigate the effects of euphorbiasteroid, a component of Euphorbia lathyris L., on adipogenesis of 3T3-L1 pre-adipocytes and its underlying mechanisms. Euphorbiasteroid decreased differentiation of 3T3-L1 cells via reduction of intracellular triglyceride (TG) accumulation at concentrations of 25 and 50 μM. In addition, euphorbiasteroid altered the key regulator proteins of adipogenesis in the early stage of adipocyte differentiation by increasing the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase. Subsequently, levels of adipogenic proteins, including fatty acid synthase, peroxisome proliferator-activated receptor-γ and CCAAT/enhancer-binding protein α, were decreased by euphorbiasteroid treatment at the late stage of adipocyte differentiation. The anti-adipogenic effect of euphorbiasteroid may be derived from inhibition of early stage of adipocyte differentiation. Taken together, euphorbiasteroid inhibits adipogenesis of 3T3-L1 cells through activation of the AMPK pathway. Therefore, euphorbiasteroid and its source plant, E. lathyris L., could possibly be one of the fascinating anti-obesity agent.

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“…Flavonoids are the main effective components responsible for their activities, such as hemostatic properties, anticancer, antitumor, antiobesity, antifertility action, antioxidation effects, and the treatment of hypertension and hemorrhoids [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination and excretion study of six flavonoids in rat after oral administration ofFructus Sophoraeextract by liquid chromatography tandem mass spectrometry

Zhi¹,

Zhang²,

Li³

et al. 2016

Acta Chromatographica

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Summary.A new, sensitive, and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS/MS) has been developed for the quantification of six flavonoids (sophoricoside, genistin, genistein, rutin, quercetin, and kaempferol) in rat bile and urine. The sample pretreatment was simple by liquid-liquid extraction. Sulfamethalazole was used as internal standard (IS). During method development, the effect of extraction volume, mobile phase composition, column temperature, and injection volume were varied to optimize sensitivity and achieve a run time as short as possible. Chromatographic separation was accomplished on a C 18 column with a simple linear gradient elution within 9 min. Full validation of the assay was in accordance with the requirement of the validation of the method in vivo and implemented including specificity, linearity, accuracy, precision, recovery, and matrix effect. This is the first report on determination of the major flavones in rat bile and urine after oral administration of Fructus Sophorae extract. The method has been used successfully in excretion studies of six major flavonoids in rat bile and urine.

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The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells

Cited by 48 publications

References 54 publications

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

ReishiMax, mushroom based dietary supplement, inhibits adipocyte differentiation, stimulates glucose uptake and activates AMPK

Euphorbiasteroid, a component of Euphorbia lathyris L., inhibits adipogenesis of 3T3‐L1 cells via activation of AMP‐activated protein kinase

Simultaneous determination and excretion study of six flavonoids in rat after oral administration ofFructus Sophoraeextract by liquid chromatography tandem mass spectrometry

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