The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver operating characteristic (TG-ROC) analysis

Xu, Huiyun; Lohr, Jürgen; Greiner, Matthias

doi:10.1016/s0022-1759(97)00128-2

Cited by 31 publications

(26 citation statements)

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“…A new diagnostic test can be evaluated with a number of different parameters, including sensitivity, specificity, accuracy, efficiency, and positive and negative predictive values (35). A critical point for such evaluations is how the cutoff point is established; therefore, in the validation of our assays we employed two well-characterized sets of serum samples with sample sizes large enough to minimize the stochastic uncertainty in cutoff selection (http://epitools.ausvet.com.au).…”

Section: Discussionmentioning

confidence: 99%

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Two novel recombinant Fasciola hepatica proteins, i.e., a ferritin (FhFtn-1) and a tegument-associated protein (FhTP16.5), were used as antigens to develop in-house enzyme-linked immunosorbent assay (ELISA) methods. The assays were optimized and validated using 152 serum samples from humans with a known infection status, including healthy subjects, patients with chronic fascioliasis, and patients with other parasitic diseases. The FhFtn-1 ELISA was shown to be 96.6% sensitive and 95.7% specific; the respective parameters for the FhTP16.5 ELISA were 91.4% and 92.4%. The performances of the FhFtn-1 and FhTP16.5 ELISAs were compared with that of an available commercial test (the DRG test) using a subset of serum samples. Our in-house tests were slightly more sensitive than the DRG test in detecting antibodies against F. hepatica, but the differences were not statistically significant. In conclusion, the present study provides evidence for the potential of the FhFtn-1 and FhTP16.5 ELISAs as diagnostic tools for human fascioliasis, as might be implemented in conjunction with standard assays for large-scale screenings in areas where the disease is endemic and for the detection of occasional cases in clinical laboratories.

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Section: Discussionmentioning

confidence: 99%

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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“…Essa análise se baseia numa curva em que são colocados os valores de corte, tendo no eixo das ordenadas a sensibilidade e no eixo das abscissas a taxa de falso positivo (ou seja, 1 menos o valor da especificidade). O ponto de corte ideal é o que permite uma maior especificidade, sem perda de sensibilidade (GREINER et al, 1995;XU et al, 1997 …”

Section: Methodsunclassified

Soroepidemiologia da doença de Newcastle em plantéis de avestruzes dos Estados da Bahia e de São Paulo

et al. 2009

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“…The cutoff point was determined by TG-ROC analysis (11,28,30), which is, briefly, a plot of the test sensitivity (Se) and specificity (Sp) against the cutoff (threshold) value (PI value), the latter being an independent variable. At the intersection point of the two graphs, known as the point of equivalence, the assay Se is equal to the assay Sp.…”

Section: Methodsmentioning

confidence: 99%

“…The hemagglutination-inhibition (HI) test is still the most widely used conventional serological method for measuring anti-NDV antibody levels in poultry sera, and it is considered the standard laboratory test for this disease (30). However, sera from other species tend to give a high incidence of falsepositive results.…”

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confidence: 99%

“…Moreover, assays with a single serum dilution are faster and more practical than serial dilution assays (7,25,26). Additionally, the determination of a suitable cutoff point in ELISA and other quantitative serodiagnostic tests becomes a useful tool of analysis for better test performance as well as reliable sensitivity and specificity, principally when no specific assumptions are made concerning the distribution of the ELISA data (30). If sensitivity and specificity are equally important, the two-graph receiver operating characteristic (TG-ROC) method is appropriate (11).…”

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confidence: 99%

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Detection and Quantification of Antibodies to Newcastle Disease Virus in Ostrich and Rhea Sera Using a Liquid Phase Blocking Enzyme-Linked Immunosorbent Assay

Sousa

Montassier

Pinto

2000

Clin Diagn Lab Immunol

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A liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutinationinhibition (HI) test was regarded as the reference method. The cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r ‫؍‬ 0.875). The two tests showed good agreement ( ‫؍‬ 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.Newcastle disease (ND) is caused by an avian paramyxovirus (APMV-1 serotype) that belongs to the genus Rubulavirus of the family Paramyxoviridae (19). Newcastle disease virus (NDV) occurs worldwide and has a considerable economic impact on the world poultry industry, ranging from losses due to disease and the expense of vaccination to the significant cost of diagnostic laboratory investigations (14). The breeding of ratites (ostriches, emus, and rheas) has expanded considerably all over the world in recent years. They are susceptible to several diseases of domestic fowl, including ND (15,20). Efforts to control and prevent ND through efficient vaccination programs and corresponding serological monitoring are constant.The hemagglutination-inhibition (HI) test is still the most widely used conventional serological method for measuring anti-NDV antibody levels in poultry sera, and it is considered the standard laboratory test for this disease (30). However, sera from other species tend to give a high incidence of falsepositive results. And although the number of nonspecific agglutination reactions can be reduced by pretreatment with heat and kaolin, these procedures decrease the sensitivity of this test (28).Indirect enzyme-linked immunosorbent assays (I-ELISA) have been developed, evaluated, and well correlated to the HI test for serodiagnosis of NDV in poultry (4,8,18). In spite of their high sensitivity, easy standardization, lack of requirement for serum pretreatment, and possible computerization of the system, these assays have the disadvantage of not being applicable to the testing of ratite sera in a single system unless anti-ratite species conjugates are used in place of an antichicken conjugate (5, 28).An APMV-1-specific monoclonal antibody blocking ELISA with the ability to test sera from exotic or wild avian species for NDV-specific antibodies in serial twofold dilutions or a single dilution has been described (9, 13). However, production and maintenance of hybridoma cells are time-consuming and sometimes expensive for laboratories with limited facilities. Moreover, assays with a single serum dilution are faster and more ...

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The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver operating characteristic (TG-ROC) analysis

Cited by 31 publications

References 2 publications

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

Soroepidemiologia da doença de Newcastle em plantéis de avestruzes dos Estados da Bahia e de São Paulo

Detection and Quantification of Antibodies to Newcastle Disease Virus in Ostrich and Rhea Sera Using a Liquid Phase Blocking Enzyme-Linked Immunosorbent Assay

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