The selective antagonism of bradykinin action on rat isolated uterus by crude Mandevilla velutina extract

Abstract

A crude aqueous alcoholic extract of Mandevilla velutina (Apocynaceae) rhizomes produced a concentration-dependent displacement to the right of the concentrationresponse curve to bradykinin (Bk) in the isolated uterus of the rat. Schild analysis of the data revealed a linear relationship (r = 0.99) and yielded a pA2 value of 3.3 + 0.08 (-log g ml-') but the slope differed significantly from unity. The anti-Bk action was of rapid onset and was reversible upon washout. Contractions induced by acetylcholine, oxytocin, angiotensin II and BaCl2 were unaffected by the extract. This represents the first report of a selective Bk antagonist of plant origin. The isolation of the active principle(s) may result in a useful pharmacological tool for elucidating the physiological and physiopathological roles of endogenous Bk.

Evidence for participation of B1 and B2 kinin receptors in formalin‐induced nociceptive response in the mouse

“…These findings provide additional evidence supporting our previous view that this compound may interfere with some stage of BK action (Calixto et al, 1985;1987;.…”

Effect of a crude extract of Mandevilla velutina on contractions induced by bradykinin and [des‐Arg9]‐bradykinin in isolated vessels of the rabbit

“…The crude aqueous/alcoholic extract of Mandevilla velutina rhizomes was prepared as described previously (Calixto et al, 1985a). Thawed rhizomes were minced and extracted with 50% ethanol-water in the proportion 1:3 (w/v), stirred mechanically at room temperature for 24 h and passed through filter paper.…”

“…Recently, we have demonstrated that an aqueous/ alcoholic extract of Mandevilla velutina rhizomes (Apocynaceae) antagonizes selectively the Bk-induced contractions in rat uterine muscle (Calixto et al, 1985a). Since we have proposed that the active principle(s) present in this extract may be interfering with the Bk receptor, it became of interest to determine whether the extract of Mandevilla velutina interacts preferentially with one subtype of Bk receptor.…”

Micropropagation and callogenesis in Mandevilla guanabarica (Apocynaceae), an endemic plant from Brazil

“…) Woodson. These species have an underground system that is widely used in folk medicine in the form of infusions or alcoholic extracts, against inflammation and snakebite (Calixto et al 1985). For optimizing the production of secondary metabolites with pharmacological properties from the underground system of Mandevilla species, micropropagation protocols have been established, using in vitro culture techniques (Handro et al 1988, Biondo et al 2004, Biondo et al 2007).…”