The selective localization of B lymphocytes in the spleen and the role of complement receptors

Abstract

The role of complement receptors on the localization of T and B cells in the spleen of mice was studied using short-term homing experiments in cobra venom factor (CoF)-treated animals. The localization ratio of B and T cells in the spleen of CoF-treated mice decreased significantly compared to control recipients. No changes could be found in the relative distribution of resident T and B cells in the spleen or other lymphoid organs of CoF-treated animals and when their spleen or lymph node cells were transferred, the localization pattern was normal. When cells were incubated in serum prior to transfer a disturbed localization ratio in the spleen of untreated recipients was observed. This was due to a blockade of complement receptors as determined by the inability of the incubated cells to form EAC rosettes. No blockade of EAC rosettes and no changes in localization ratios upon transfer could be observed when the cells were incubated in functionally C3-depleted serum. The results suggest a role for the complement-receptor on B cells in the initial localization in the spleen, whereas no influence upon the selective localization in high endothelial venules-bearing organs was found.

Asplenic-hyposplenic Overwhelming Sepsis: Postsplenectomy Sepsis Revisited

“…If the receptors are blocked, the B cells do not interact properly with the splenic reticular cells and endothelial cells, resulting in faulty B-cell homing. T cells are not adversely affected and migrate properly within the spleen [22].…”

A Comparative Study of Normal B Cells and the EBV‐Positive Burkitt's Lymphoma Cell Line, Raji, as Activators of the Complement System

“…In conclusion, it would appear that the instrinsic capacity of CR2 to act as a site for the generation of an AP C3-convertase is just as pronounced for normal B cells, as it is for Burkitt's lymphoma cells and malignant B cells from patients with chronic lymphocytic leukaemia [10]. These observations raise the possibility that AP activation, and the consequent C3 fragment deposition, may be of physiological significance, either in terms of B cell trafficking [20] or in the modulation of B cell reactivity with C3 fragment-bearing antigen [21]. Furthermore, the data presented here raise an additional question, namely, whether the type of C3 fragments deposited may be of significance in influencing normal or diseased B cell activity.…”

Equine splenectomy: Do we understand the consequences?

“…In addition to antibody production, the spleen functions as a homing hub for B cells within the splenic follicles as long as cell surface receptors for complement are present. If these receptors are blocked or not present because the spleen is absent, B cells do not interact properly with splenic reticular cells and endothelial cells, resulting in insufficient homing of B cells and a deficient immune response (Kraal et al 1985;Hooper et al 1986).…”