The development of fast, specific, ultrasensitive, and accurate techniques for the early detection of important pathogens has been spurred by growing concerns about environmental safety, food safety, and nosocomial infection. Many bacteria, which may be found in foods, water, and medical equipment, cause diseases in humans and plants. In recent years, antibiotic resistance has arisen as a significant concern, as has their contamination as a result of unregulated use. As a result, there is a greater need to quickly establish reliable, simple, and sensitive techniques for evaluating antibiotics and their residues. The majority of bacterial detection procedures are lengthy and take days to provide a result. Aptamers appear to be a potential option for detecting bacteria in food items rapidly and reliably. Because of their high sensitivity, specificity, and selectivity, aptamer-based biosensors (aptasensors) attracted a lot of interest. This article provides a summary of newly discovered antibiotic detection aptasensors. This paper discusses the use of several aptamer assays to identify antibiotics such as sulfonamides, tetracyclines, lincosamide, (fluoro)quinolones, chloramphenicol, anthracyclines, aminoglycosides, and β-lactams.