The assessment of pain relies mostly on methods that require a person to communicate. However, for people with cognitive and verbal impairments, existing methods are not sufficient as they lack reliability and validity. To approach this problem, recent research focuses on an objective pain assessment facilitated by parameters of responses derived from physiology, and video and audio signals. To develop reliable automated pain recognition systems, efforts have been made in creating multimodal databases in order to analyze pain and detect valid pain patterns. While the results are promising, they only focus on discriminating pain or pain intensities versus no pain. In order to advance this, research should also consider the quality and duration of pain as they provide additional valuable information for more advanced pain management. To complement existing databases and the analysis of pain regarding quality and length, this paper proposes a psychophysiological experiment to elicit, measure, and collect valid pain reactions. Participants are subjected to painful stimuli that differ in intensity (low, medium, and high), duration (5 s / 1 min), and modality (heat / electric pain) while audio, video (e.g., facial expressions, body gestures, facial skin temperature), and physiological signals (e.g., electrocardiogram [ECG], skin conductance level [SCL], facial electromyography [EMG], and EMG of M. trapezius) are being recorded. The study consists of a calibration phase to determine a subject's individual pain range (from low to intolerable pain) and a stimulation phase in which pain stimuli, depending on the calibrated range, are applied. The obtained data may allow refining, improving, and evaluating automated recognition systems in terms of an objective pain assessment. For further development of such systems and to investigate pain reactions in more detail, additional pain modalities such as pressure, chemical, or cold pain should be included in future studies. Recorded data of this study will be released as the "X-ITE Pain Database". Video Link The video component of this article can be found at https://www.jove.com/video/59057/ 14,15 based solely on one of the signal sets 10,11,12,13,14,15 as well as on a combination/fusion 16,17,19 of the sets. The abovementioned modalities react almost autonomously to stressful stimuli such as pain. Using them has the advantage that they do not require a person's ability to report her/his pain. Such individuals would greatly benefit from an objective pain recognition system which incorporates such modalities. Data sets consisting of reactions to elicited pain provide precious information for analyzing pain patterns and developing practical applications for detecting and monitoring pain. Amongst others, Walter et al. 20 created the "BioVid Heat Pain Database", a multimodal database that is publicly available and provides data from short-time induced painful heat stimuli and corresponding psychophysiological and visual reactions. The "SenseEmotion