The Sensitivity of Orchid Seedlings to Nutritional Ions

Wynd, F. L.

doi:10.2307/2394424

Cited by 7 publications

(1 citation statement)

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“…½ MS contains low amount of calcium (220 mg l -1 ) compared to other media used and this could be the optimum level for the seeds to germinate, whereas an increase or decrease from this level showed lesser germination. It is suggested that, orchid seedlings might react favorably to an increase in nitrogen as ammonium ion than the nitrate form in seedling growth (Wynd, 1933) since the uptake of ammonium is faster than that of nitrate. In this experiment, nitrogen source in both the forms are noticeably effective in germination and development.…”

Section: Discussionmentioning

confidence: 99%

Influence of nutritional media and photoperiods on in vitro asymbiotic seed germination and seedling development of Dendrobium aqueum Lindley

Parthibhan¹

2012

Afr. J. Plant Sci.

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Dendrobium aqueum Lindley is an epiphytic orchid decreasing in nature owing to environmental disturbance and inherent lower germination rates of mature seeds. To conserve this species, in vitro asymbiotic media and photoperiod screening were experimented. Twenty asymbiotic orchid seed germination media were examined to get the optimal medium for germination and seedling development under 16/8 hrs photoperiod. Highest seed germination occurred in 1 / 2 MS medium (93.41%) followed by mMS (91.04%) and m ½ MS (90.23%) in 6 to 7 weeks. The biggest protocorms were observed in MS medium (788.8 µm) followed by ½ MS (615.60 µm). The yellowish embryos during germination exhibit varied colours from yellow to pale green, dark green, yellow, brown, black and white. MS, ½ MS, mMS, ½ mMS, and ¼ MS media only promote fully developed seedlings within 126 to 143 days. The influence of five different photoperiods with four media was also examined separately. 24/0 hrs (L/D) photoperiod provide highest germination (97.75%) on MS followed by ½ MS (96.01%). Dark Photoperiod 0/24 h (L/D) supports up to protocorm stage but with poor seedling growth. The well rooted seedlings were hardened and successfully reintroduced in its natural habitat with 56% survival rate.

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Section: Discussionmentioning

confidence: 99%