The separation and evaluation of puric bases and their nucleosides and nucleotides by thin-layer chromatography

Binding of ¹⁴C-ADP by Thrombasthenic Platelet Membranes

“…Following analysis by thin-layer chromatography according to Chivot et al [7], only 14C-ADP could be detected on the radiochromatogram showing that the bound UC-ADP was not metabolized within the 60 min incubation time of the experiment.…”

“…The bound radioactivity was extracted by incubating the membranes at 37 °C with 1 n hydrochloric acid. The eluate was analyzed by radio chromatography on PEI cellulose plates in two solvent systems as described by C hivot et al [7].…”

Developmental Change and Hormonal Control of β-Glucosidase Activity in Rat Kidney

“…Liberated p-nitrophenol was then deter mined by absorbance at 410 nm and enzyme activity was expressed as units (1 nmol p-nitrophenol/min) per milligram protein. (3-Glucuronidase, N-acetyl-/3-glucosaminidase and (3-galactosidase activities were assayed under the same conditions as described above except for the substrate and buffer system (2). Reaction mixtures for these assays contained PNP derivatives (PNP glucuronide, 5 mmol/1; PNP N-acetylglucosaminide, 2.5 mmol/1; PNP galactopyranoside, 1 mmol/1; PNP phosphate, 10 mmol/1) as substrates and 50 mmol/1 citrate buffer (pH 4.0).…”