The separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection

Ochs, Scott D.; Westfall, Thomas C.; Macarthur, Heather

doi:10.1016/j.jneumeth.2004.08.010

Cited by 27 publications

(32 citation statements)

References 26 publications

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“…The Published HPLC Procedures [13] The HPLC analysis was performed using AKTA purifier HPLC system (Amersham, Sweden) with a UV detector and a reversed-phase column (DENALI TM Monomeric RPC C18 4.6 mm 9 250 mm, 120 Å pore size VYDAC 238DE TM Series, Grace Vydac, USA) and analyzed under the control of UNICON 4.11 program. The published HPLC procedure used one elution buffer (8% methanol (v/v), 0.1 M sodium phosphate, 0.2 mM sodium octyl sulfate, 0.1 mM EDTA, adjusted to pH 2.8 with phosphoric acid).…”

Section: Incubation Of Da or Am In Solutionsmentioning

confidence: 99%

“…We initially used a recently published HPLC method [13] which was used to specifically separate and detect AM in HPLC chromatogram. However, we found that the resulting AM peaks always overlapped with DA peaks in HPLC chromatograms and there was also excursion of DA and AM peaks in the chromatogram (Fig.…”

Section: Well Separation Of Am and Da Peaks By Improved Hplc A Proceduresmentioning

confidence: 99%

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Roles of Glutathione (GSH) in Dopamine (DA) Oxidation Studied by Improved Tandem HPLC Plus ESI-MS

Zhou

Lim

2008

Neurochem Res

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In this study, new procedure with improved tandem HPLC plus ESI-MS was utilized to decipher the protective role of glutathione (GSH) against dopamine (DA) oxidation. We demonstrated that auto-oxidation of DA could produce aminochrome (AM, a cyclized DA quinone), which could be effectively abrogated by reductants, especially by GSH. Furthermore GSH was demonstrated to be able to conjugate with AM to form various conjugates via condensation reactions without enzymatic catalysis. The GSH-AM conjugates tend to aggregate, possibly mediated by conjugated AM structures, but could be inhibited by GSH. We hypothesized that proteins conjugated by AM might facilitate Lewy body formation of Parkinson's disease (PD) in dopaminergic neurons via similar polymerization. We proposed that GSH could protect dopaminergic neurons against DA-induced toxicity via various mechanisms. The imbalance between DA oxidation and GSH protective capacity could be a key factor contributing to PD. Strategies to use GSH analogues, GSH inducers or to control DA oxidation might work to control PD onset and development.

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Section: Incubation Of Da or Am In Solutionsmentioning

confidence: 99%

Section: Well Separation Of Am and Da Peaks By Improved Hplc A Proceduresmentioning

confidence: 99%

Roles of Glutathione (GSH) in Dopamine (DA) Oxidation Studied by Improved Tandem HPLC Plus ESI-MS

Zhou

Lim

2008

Neurochem Res

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“…Serum cytokine levels, including TNF-␣, IL-1␣, IL-1␤, IL-2, IL-4, IL-6, IL-10, INF-␥, and granulocyte macrophage colony-stimulating factor, three migratory inhibitory proteins (MIP-1␣, MIP-2, and MIP-3␣), monocyte chemoattractant protein-1, and RANTES (regulated on activation, normal T-cell expressed and secreted) were measured by using the Searchlight Proteome Array Multiplex system (Pierce, Rockford, IL). Plasma nonoxidized and oxidized catecholamines (norepinephrine and epinephrine) were measured by HPLC with electrochemical detection, as previously described (24,31).…”

Section: M40401 Experimentsmentioning

confidence: 99%

Severity of sepsis alters the effects of superoxide anion inhibition in a rat sepsis model

Cui

Parent²,

Macarthur³

et al. 2004

Journal of Applied Physiology

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of sepsis alters the effects of superoxide anion inhibition in a rat sepsis model. J Appl Physiol 97: 1349 -1357, 2004. First published May 28, 2004 10.1152 10. /japplphysiol.01161.2003 analysis showed that selective inhibitors of five different host inflammatory mediators administered for sepsis, although beneficial with severe sepsis and high-control mortality rates, were ineffective or harmful with less severe sepsis. We hypothesized that severity of sepsis would also influence inhibition of superoxide anion, another inflammatory mediator. To test this, 6-h infusions of M40401, a selective SOD mimetic, or placebo were given to antibiotic-treated rats (n ϭ 547) starting 3 h after challenge with differing doses of intravenous Escherichia coli designed to produce low-or high-control mortality rates. There was a positive and significant (P ϭ 0.0008) relationship between the efficacy of M40401 on survival rate and control mortality rates. M40401 increased or decreased the log (odds ratio of survival) (means Ϯ SE), dependent on whether control mortality rates were greater or less than the median (66%) (ϩ0.19 Ϯ 0.12 vs. Ϫ0.25 Ϯ 0.10, P ϭ 0.01). In a subset of animals examined (n ϭ 152) at 9 h after E. coli challenge, M40401 increased (mean effect Ϯ SE compared with control) mean arterial blood pressure (8 Ϯ 5 mmHg) and decreased platelets (Ϫ37 Ϯ 22 cells ϫ 10 3 /ml) with high-control mortality rates but had opposing effects on each parameter (Ϫ3 Ϯ 3 mmHg and 28 Ϯ 19 cells ϫ 10 3 /ml, respectively) with low rates (P Յ 0.05 for the differing effects of M40401 on each parameter with high-vs. low-control mortality rates). A metaregression analysis of published preclinical sepsis studies testing SOD preparations and SOD mimetics showed that most (16 of 18) had control mortality rates Ͼ66%. However, across experiments from published studies, these agents were less beneficial as control mortality rate decreased (P ϭ 0.03) in a relationship not altered (P ϭ not significant) by other variables associated with septic challenge or regimen of treatment and which was similar, compared with experiments with M40401 (P ϭ not significant). Thus, in these preclinical sepsis models, possibly related to divergent effects on vascular function, inhibition of superoxide anion improved survival with more severe sepsis and high-control mortality rates but was less effective or harmful with less severe sepsis. Extrapolated clinically, inhibition of superoxide anion may be most efficacious in septic patients with severe sepsis and a high risk of death. septic shock; treatments; superoxide dismutase mimetic; M40401

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“…While simple methods solely employ UV detection (what is suitable for analytes with extended chromophores), better results can be achieved via combination of different, orthogonal detection regimes, such as fluorescence or electrochemistry. The latter was fielded by Ochs et al (2005) resulting in separation and quantification of different aminochromes, i.e. dopaminochrome, noradrenochrome or adrenochrome, with high sensitivity in nanomolar concentration ranges.…”

Section: Stability-indicating Analytical Approaches For Epi and Ne Qumentioning

confidence: 99%

Ficts and facts of epinephrine and norepinephrine stability in injectable solutions

Hoellein

Holzgrabe

2012

International Journal of Pharmaceutics

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The separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection

Cited by 27 publications

References 26 publications

Roles of Glutathione (GSH) in Dopamine (DA) Oxidation Studied by Improved Tandem HPLC Plus ESI-MS

Roles of Glutathione (GSH) in Dopamine (DA) Oxidation Studied by Improved Tandem HPLC Plus ESI-MS

Severity of sepsis alters the effects of superoxide anion inhibition in a rat sepsis model

Ficts and facts of epinephrine and norepinephrine stability in injectable solutions

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