The separation of ketosteroids by partition chromatography using nitromethane as stationary phase

Jones, J. K. N.; Stitch, S. R.

doi:10.1042/bj0530679

Cited by 23 publications

(4 citation statements)

References 16 publications

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“…Cig-STEROIDS Adsorption chromatography (cf. 99, 292) has been in use for some time for the identification and quantitative estimation of the relatively large amounts of 17-ketosteroids in the urine, but partition chromatography is rapidly gaining favor for qualitative (341,353,401,292,257,108,120,217) and quantitative (41,340,211,99,323) analysis. It is a valuable tool for the study of the biosynthesis (56,15,117,354) and tissue metabolism (238,329,450,451,449,223) of androgens, and for the study of enzymatic reactions (414,415,114).…”

Section: B Quantitative Methodsmentioning

confidence: 99%

“…Morris and Williams (276) separated dehydrocorticosterone, corticosterone, and 17-hydroxydeoxy-corticosterone7 on Supercel columns containing 1,2-ethanediol with a petroleum ether-toluene (1:4) mixture. In the separation of ketosteroids according to Jones and Stitch (211) the stationary phase is nitromethane on a silicic acid column and the mobile phase is 3 per cent chloroform in petroleum ether. With columns of sufficient length such closely related steroids as androstan-3/3-ol-17-one and A5-androsten-3/3-ol-17-one can be separated.…”

Section: -Acidsmentioning

confidence: 99%

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Partition Chromatography Of Steroids

Heftmanń¹

1955

Chem. Rev.

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Section: B Quantitative Methodsmentioning

confidence: 99%

Section: -Acidsmentioning

confidence: 99%

Partition Chromatography Of Steroids

Heftmanń¹

1955

Chem. Rev.

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“…The production of artifacts by acid hydrolysis during isolation of sterols from human urine has been described (cf. Marrian, 1951;Jones & Stitch, 1953;Brown, 1955).…”

Section: Dosementioning

confidence: 99%

The metabolism of butylated hydroxyanisole in the rabbit

Dacre¹,

Denz²,

Kennedy

1956

Biochemical Journal

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PREVENTION OF SWELLNG OF LIVER MITOCHONDRIA 777 and by inorganic orthophosphate. Added Na+, K+, CFand S042ions in the range 1-10 mm are without effect, and citrate, oxalate and ethylenediaminetetraacetate protect mitochondria from swelling. 4. The effects of the nucleotides, Mg2+ and Mn2+ ions on the water content of mitochondria are also appreciable in a medium consisting of only 40 mm sucrose. In this medium AMP is more effective than adenosine diphosphate (ADP), which is better than ATP, apparently because of the inhibitory effect of the inorganic phosphate liberated during incubation. All three nucleotides are broken down to adenosine and inorganic phosphate, and this breakdown is more rapid in the presence of Mg2+, Mn2+ and Ca2+ ions either alone or in mixtures. 5. Adenosine 3'-phosphate, inosine 5'-phosphate, guanosine 5'-phosphate, uridine 5'-phosphate, flavin mononucleotide, adenosine and inosine were without effect on the water content ofmitochondria. 6. It is concluded that the agents which prevent swelling act on the structure of the mitochondria in ways which are not yet understood.

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“…Our initial work considered only those dosage forms in vegetable oils other than castor oil, because the latter, which consists primarily of the glycerides of hydroxy fatty acids, has properties quite different from those of other vegetable oils. Jones and Stitch (12) previously reported separation of mixtures of steroids in biological material by partition chromatography, using the polar organic solvent nitromethane as immobile phase on a silicic acid support and 3% CHC13 in petroleum ether as the mobile phase. Wolff (13) in our laboratories isolated progesterone from its oil injection with nitromethane as the immobile phase, using Celite 545 as support and n-heptane as the mobile phase.…”

mentioning

confidence: 99%

Analysis of oil‐based pharmaceuticals

Smith

1972

J Americ Oil Chem Soc

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Oils of animal, mineral and vegetable origin are used in the formulation of pharmaceuticals. In most cases the analysis of these pharmaceuticals requires the separation of the drug substance from the oil components prior to its quantitation, the required degree of separation depending on the specificity of the quantitation method. When the physical properties of the drug substance are quite similar to those of components of the oil, the separation of the drug becomes complex. Column partition chromatography provides an excellent means of separating steroids from the vegetable oil vehicles used in injectables. Both reverse phase and direct phase techniques are applied to the separation of the steroids from the glyceride, stern and triterpenoid fractions of the oil. Illustrations are provided to demonstrate the application of thin layer chromatography, paper chromatography, gas chromatography, gel permeation chromatography and adsorption chromatography in the analysis of oil solutions, oil-based dermatological preparations and suppositories.

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The separation of ketosteroids by partition chromatography using nitromethane as stationary phase

Cited by 23 publications

References 16 publications

Partition Chromatography Of Steroids

Partition Chromatography Of Steroids

The metabolism of butylated hydroxyanisole in the rabbit

Analysis of oil‐based pharmaceuticals

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