The separation of oligonucleotides of baker's-yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography

Gorbulev, V.G.; Barciszewski, Jan; Axelrod, V.D.

doi:10.1042/bj1630409

Search citation statements

Order By: Relevance

Paper Sections

Select...

Discussion1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1977

1979

Publication Types

Select...

Article2

Book1

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Often the components were identified from their chromatographic mobilities comparing with the mobilities of markers because of lower sensitivity of spectral method. The fingerprinting system developped in our laboratory [16] allows to separate practically all fragments of guanylo-RNase and pancreatic RNase digests of tRNA. Moreover the electrophoretic mobilit;y of oligonucleotides happened to be higher at pH=1.5-1.55 than at pH=1.7.…”

Section: Discussionmentioning

confidence: 99%

Primary structure of baker's yeast tRNA_2b^Val

Gorbulev¹,

Axelrod²,

Bayev³

1977

Nucl Acids Res

View full text Add to dashboard Cite

The minor form of valine tRNA from baker's yeast-tRNAVal 2b--purified by column chromatography was completely digested with guanylo-RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic RNase, and their complete guanylo-RNase and pancreatic RNase digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA Val 2b was reconstructed.

show abstract

Section: Discussionmentioning

confidence: 99%