The septin family of GTPases: architecture and dynamics

Weirich, Christine S.; Erzberger, J.P.; Barral, Yves

doi:10.1038/nrm2407

Cited by 316 publications

(317 citation statements)

References 115 publications

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“…The annulus structure was completely absent in all spermatozoa analyzed. c Western blot analysis for septin 4 which was not detected in the gel; PC positive control Septin proteins belong to the GTPase superclass of P loop NTPases [10][11][12]. Sept4 null mice show the reproductive phenotypes similar to a subset of asthenozoospermic patients [7,8].…”

Section: Discussionmentioning

confidence: 99%

Relationship between absence of annulus and asthenozoospermia in Iranian men

Hosseinifar

Shafipour

Modarresi

et al. 2014

J Assist Reprod Genet

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Purpose To find a relationship between absence of annulus and asthenozoospermia in Iranian men. Methods In the present study, semen samples from 100 asthenozoospermic and 20 normozospermic patients were analyzed for sperm concentration and motility. Spermatozoa were immunostained for the two septin subunits Sept4 and Sept7. The absence of the annulus structure was confirmed by transmission electron microscopy and western blot analysis for septin 4. DNA sequencing for all coding exons of SEPT12 was performed for a patient using peripheral blood sample. Results Specific antibodies for SEPT4 and SEPT7 consistently labeled the annuli in spermatozoa from all of the 20 normozospermic men, while in one of 100 patients with asthenozoospermia, 75 % of sperms lacking septin 4 or septin 7 proteins at the annulus. It was shown that the structural defect in annulus formation is not caused by point mutation of SEPT12 gene. ConclusionsIn conclusion, the results of this study demonstrated that the frequency of the absence of annulus in asthenozoospermic sample of Iranian population has a low frequency and could not be assume as a diagnostic marker for classifying asthenozoospermic patients.

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Section: Discussionmentioning

confidence: 99%

Relationship between absence of annulus and asthenozoospermia in Iranian men

Hosseinifar

Shafipour

Modarresi

et al. 2014

J Assist Reprod Genet

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“…The crystal structure of the SEPT2-SEPT6-SEPT7 complex was recently solved and revealed that septins form non-polar filaments unlike actin and microtubules [Sirajuddin et al, 2007]. Despite the significance of these structural insights [Barral and Kinoshita, 2008;Weirich et al, 2008], it remains to be determined how septins function as a distinct component of the cytoskeleton.…”

Section: Septins: a Novel Component Of The Cytoskeletonmentioning

confidence: 99%

Cytoskeleton rearrangements during Listeria infection: Clathrin and septins as new players in the game

Mostowy

Cossart

2009

Cell Motil. Cytoskeleton

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The study of an infection process can reveal how microbes exploit the host, and can illuminate unknown host cellular functions. Invasive pathogens have evolved efficient strategies to promote their internalization within normally non-phagocytic host cells. The so-called ''zippering'' bacteria present to host cell receptors molecules that mimic endogenous ligands, thereby inducing specific intracellular signaling cascades ultimately resulting in actin polymerization and uptake. Here we review how the bacterial pathogen Listeria monocytogenes enters into cells, and present a series of studies revealing that in addition to actin rearrangements this bacterium exploits the clathrin-mediated endocytosis machinery together with septins, a novel cytoskeleton element. The challenge is now to decipher how all of these components orchestrate themselves to permit entry into normally non-phagocytic cells. Cell Motil. Cytoskeleton 66: 816-823, 2009. '

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“…8). Mammalian septins are evolutionary conserved proteins that form filaments associated with cytoskeleton organization, membrane dynamics, mitosis, cytokinesis, and cell cycle progression (9,10). Recently, accumulating data suggest that some septin family members also participate in the pathogenesis of neoplasia (11) and neurodegenerative diseases (12).…”

Section: Introductionmentioning

confidence: 99%

Targeted Knockdown of SEPT9_v1 Inhibits Tumor Growth and Angiogenesis of Human Prostate Cancer Cells Concomitant with Disruption of Hypoxia-Inducible Factor-1 Pathway

Sharon

Golan

Mabjeesh

2010

Molecular Cancer Research

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Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor in the hypoxic response pathway. We recently identified a novel interaction between HIF-1α and the mammalian septin family member, septin 9 protein, isoform 1 (SEPT9_i1), a protein product of septin 9 transcript variant 1 (SEPT9_v1). Septins are a highly conserved family of GTP-binding cytoskeletal proteins that are implicated in multiple cellular functions, including oncogenesis. SEPT9_i1 binds and stabilizes HIF-1α protein and stimulates HIF-1 transcriptional activity by preventing its RACK1-mediated ubiquitination and degradation. SEPT9_i1-HIF-1 activation promotes tumor growth and angiogenesis. The effect of SEPT9_v1 silencing in prostate cancer cells was studied. SEPT9_v1 stable knockdown was generated in PC-3 cells using a specific shRNA. SEPT9_v1 silencing reduced HIF-1α protein expression and inhibited HIF-1 transcriptional activity. SEPT9_v1 knockdown affected cell morphology, deregulated cell cycle, and decreased migration. The antiproliferative effect of shSEPT9_v1 was abolished in HIF-1α knockout colon cancer cells. In vivo, SEPT9_i1 depletion reduced HIF-1α protein expression, cellular proliferation, tumor growth, and angiogenesis. These results provide new insights and validation for applying SEPT9_v1 as a potential target for antitumor therapy by interrupting the HIF-1 pathway. Mol Cancer Res; 8(5); 643-52. ©2010 AACR.

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The septin family of GTPases: architecture and dynamics

Cited by 316 publications

References 115 publications

Relationship between absence of annulus and asthenozoospermia in Iranian men

Relationship between absence of annulus and asthenozoospermia in Iranian men

Cytoskeleton rearrangements during Listeria infection: Clathrin and septins as new players in the game

Targeted Knockdown of SEPT9_v1 Inhibits Tumor Growth and Angiogenesis of Human Prostate Cancer Cells Concomitant with Disruption of Hypoxia-Inducible Factor-1 Pathway

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