The septins: roles in cytokinesis and other processes

Longtine, Mark S.; DeMarini, Douglas J.; Valencik, Maria L.; Al-Awar, Omayma; Fares, Hanna; Virgilio, Claudio De; Pringle, John R.

doi:10.1016/s0955-0674(96)80054-8

Cited by 462 publications

(540 citation statements)

References 123 publications

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“…Bradeion belongs to the septin family genes, 5 which are evolutionarily conserved cytoskeletal GTPases involved in cytokinesis and other cellular processes. The best conserved region is an ATP /GTP binding site ( P-loop ), called Walker's A box.…”

Section: Discussionmentioning

confidence: 99%

“…Through these processes, we have identified a variety of novel genes, and one of these is Bradeion. 1 The Bradeion gene belongs to the mammalian septin family genes, 5 and originally was discovered from human brain cDNA by expression screening using the monoclonal antibody CE5. 6 Interestingly, Bradeion is expressed specifically in two human cancers, colorectal cancer and malignant melanoma, with ''all or none'' expression profile and no point mutations.…”

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confidence: 99%

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Impaired expression of a human septin family gene Bradeion inhibits the growth and tumorigenesis of colorectal cancer in vitro and in vivo

et al. 2002

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We have identified a novel human septin family gene Bradeion, which is specifically expressed in human colorectal cancer and malignant melanoma. In order to analyze the implications of tumor -specific gene expression, ribozymes and its derivatives were specifically designed and transfected into various colorectal adenocarcinoma cell lines for Bradeion inactivation. We constructed ribozyme expression plasmids controlled by a human tRNA Val promoter, and both hammerhead ribozyme and its allosteric derivative maxizyme were used for two different forms of Bradeion mRNA. The sequence -specific cleavage of Bradeion mRNA resulted in significant growth inhibition and G2 arrest in human cancer cell lines, detected by flow cytometry analysis. In addition, in vivo mice studies demonstrated marked tumor growth suppression by the Bradeion -specific ribozymes. Thus, the tumor -specific and selective marker Bradeion also provides valuable tools as a potential target for colorectal cancer therapy.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Impaired expression of a human septin family gene Bradeion inhibits the growth and tumorigenesis of colorectal cancer in vitro and in vivo

et al. 2002

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“…The role of septins during cytokinesis in yeast, fl ies and humans is well-documented [9]. The use of PCR-based cloning and genomics has revealed that A. nidulans possesses fi ve members of the septin family (aspA-E), each belonging to a specifi c orthologous group [10].…”

Section: Septinsmentioning

confidence: 99%

Septum formation in Aspergillus nidulans

Harris

2001

Current Opinion in Microbiology

105

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Filamentous fungi form multicellular hyphae that are partitioned by septa. In A. nidulans, septum formation requires the assembly of a septal band following the completion of mitosis. Recent observations show that this band is a dynamic structure composed of actin, a septin and a formin. In addition, assembly is dependent upon a conserved protein kinase cascade that regulates mitotic exit and septation in yeast. Hyphal differentiation may refl ect the regulation of this cascade by cyclin-dependent kinase activity. In this review, the dynamics and regulation underlying the assembly of the septal band are discussed.

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“…We next investigated the effect of abolishing SKM1 activity in mutants affected in the septin-encoding gene CDC10 (Longtine et al, 1996), in the Rho1p-GAP-encoding gene BEM2 (Peterson et al, 1994), and in the Rho-like protein coded by CDC42, by crossing the Y881, 918-8A and DJTD2-16D strains, respectively, to strains carrying the skm1⌬::URA3 mutation. Double-mutant segregants displayed the same morphogenetic defects as the single mutant strains.…”

Section: Skm1p Does Not Interact Genetically With Other Morphogeneticmentioning

confidence: 99%

**Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK‐like protein kinase**

Martı́n

Mendoza

Rodrı́guez-Pachón

et al. 1997

Molecular Microbiology

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SummarySte20/PAK serine/threonine protein kinases have been suggested as playing essential roles in cell signalling and morphogenesis as potential targets of Cdc42 and Rac GTPases. We have isolated and characterized the Saccharomyces cerevisiae SKM1 gene, which codes for a novel member of this family of protein kinases. The amino acid sequence analysis of Skm1p revealed the presence of a PH domain and a putative p21-binding domain near its amino terminus, suggesting its involvement in cellular signalling or cytoskeletal functions. However, deletion of SKM1 produced no detectable phenotype under standard laboratory conditions. Moreover, disruption of each of the two other S. cerevisiae Ste20/PAK-like kinase-encoding genes, STE20 and CLA4, in skm1 backgrounds, showed that Skm1p is not redundant with Ste20p or Cla4p. Interestingly, overexpression of SKM1 led to morphological alterations, indicating a possible role for this protein in morphogenetic control. Furthermore, overproduction of Skm1p lacking its N-terminus caused growth arrest. This effect was also seen when similarly truncated versions of Ste20p or Cla4p were overexpressed. We further observed that overproduction of this C-terminal fragment of Skm1p complements the mating defect of a ste20 mutant strain. These results suggest that the N-terminal domains of S. cerevisiae Ste20/ PAK-like protein kinases share a negative regulatory function and play a role in substrate specificity.

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The septins: roles in cytokinesis and other processes

Cited by 462 publications

References 123 publications

Impaired expression of a human septin family gene Bradeion inhibits the growth and tumorigenesis of colorectal cancer in vitro and in vivo

Impaired expression of a human septin family gene Bradeion inhibits the growth and tumorigenesis of colorectal cancer in vitro and in vivo

Septum formation in Aspergillus nidulans

**Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK‐like protein kinase**

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