The Sequence of 16S rRNA From<i>Mycoplasma</i>Strain PG50

Frydenberg, Jane; Christiansen, Claus

doi:10.1089/dna.1985.4.127

Cited by 31 publications

(13 citation statements)

References 27 publications

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“…In (27) to hybridize to the 16S probe from Mycoplasma sp. strain PG50 (8). However, the 23S rDNA probe hybridized to different fragments (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Kim and Chan (16), however, have estimated the size of one C. jejuni strain (TGH9011) to be 1.9 Mb by field inversion gel electrophoresis. Nuijten et al (27) have constructed a genome map of C. jejuni 81116 and determined the locations of the flagellar genes (flaA and flaB) with a homologous DNA probe and those of the 16S rRNA genes with a heterologous DNA probe from a mycoplasma (8). PFGE has also proved useful for comparison of C. jejuni and C. coli genome DNA for epidemiologic studies (51).…”

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confidence: 99%

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Genome maps of Campylobacter jejuni and Campylobacter coli

et al. 1992

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Little information concerning the genome of either Campylobacter jejuni or Campylobacter coli is available. Therefore, we constructed genomic maps of C. jejuni UA580 and C. coli UA417 by using pulsed-field gel electrophoresis. The genome sizes of C. jejuni and C. coli strains are approximately 1.7 Mb, as determined by SalI and SmaI digestion (N. Chang and D. E. Taylor, J. Bacteriol. 172:5211-5217, 1990). The genomes of both species are represented by single circular DNA molecules, and maps were constructed by partial restriction digestion and hybridization of DNA fragments extracted from low-melting-point agarose gels. Homologous DNA probes, encoding the flaAB and 16S rRNA genes, as well as heterologous DNA probes from Escherichia coli, Bacillus subtilis, and Haemophilus influenzae, were used to identify the locations of particular genes. C. jejuni and C. coli contain three copies of the 16S and 23S rRNA genes. However, they are not located together within an operon but show a distinct split in at least two of their three copies. The positions of various housekeeping genes in both C. jejuni UA580 and C. coli UA417 have been determined, and there appears to be some conservation of gene arrangement between the two species.

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“…In (27) to hybridize to the 16S probe from Mycoplasma sp. strain PG50 (8). However, the 23S rDNA probe hybridized to different fragments (Fig.…”

Section: Resultsmentioning

confidence: 96%

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confidence: 99%

Genome maps of Campylobacter jejuni and Campylobacter coli

et al. 1992

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“…Sequence analysis of certain genes is, therefore, an extremely useful complement or alternative to conventional methods for identification and for phylogenetic studies. Complete and partial sequences of the 16S rRNA genes from the rrnA and rrnB operons have been determined for some of the members of the M. mycoides cluster (19,27,41,44,56).…”

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confidence: 99%

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

et al. 1996

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The so-called Mycoplasma mycoides cluster consists of six species or subspecies of mycoplasmas (Mollicutes). These species are pathogenic for ruminants and some of them are of great concern in veterinary medicine. The members of the M. mycoides cluster have two rRNA operons (rrnA and rrnB). The nucleotide sequences of the 16S rRNA genes of 10 strains, representing all of the known species and subspecies of the M. mycoides cluster, were determined by direct automated solid-phase DNA sequencing. The sequences of both rRNA operons were determined by a novel strategy involving in vitro amplification by PCR with one operon-specific primer pair and one general primer pair. Interestingly, sequence differences (polymorphisms) between the two operons were observed for all strains. Two strains of M. capricolum subsp. capripneumoniae were sequenced, and 15 polymorphisms were found in the type strain (F38) and 17 polymorphisms were found in the other strain (4/2LC). Eight polymorphisms were found in the 16S rRNA genes of the M. mycoides subsp. mycoides smallcolony type, and sequence length variations in a poly(A) region were observed in the 16S rRNA genes of the two operons of this species. Secondary-structure analysis showed that polymorphisms were present in both stem and loop regions. The nucleotide substitutions in the polymorphic sites of the stem regions often resulted in a change from a canonical to a noncanonical base pairing or vice versa. A compensatory mutation was never observed in the other nucleotide of the base pair. Phylogenetic analysis based on the 16S rRNA sequences indicated that Mycoplasma sp. strain PG50 should be included in the M. capricolum species group. Furthermore, the 16S rRNA sequences of M. mycoides subsp. capri and the M. mycoides subsp. mycoides large-colony type were 99.9% identical. We therefore suggest that these species be reclassified in a common species group (for instance, "Mycoplasma capri") distinct from the M. mycoides subsp. mycoides small-colony type, which formed an intermediate branch between the M. capricolum species group and the M. capri species group.Many species belonging to the class Mollicutes (trivial name, mycoplasmas) are pathogenic and of great economic concern in livestock production. There is one interesting group of six closely related mycoplasmas named the Mycoplasma mycoides cluster, consisting of several ruminant pathogens (13,49,50). This group comprises the following species, subspecies, or strains: M. capricolum subsp. capricolum (formerly M. capricolum), M. capricolum subsp. capripneumoniae (formerly Mycoplasma sp. strain F38), M. mycoides subsp. capri, the M. mycoides subsp. mycoides large-colony (LC) and small-colony (SC) types, and Mycoplasma sp. strain PG50.The best-known species in the M. mycoides cluster is perhaps the M. mycoides subsp. mycoides SC type, which is the causative agent of contagious bovine pleuropneumonia. This disease has been known since the 18th century, and the organism was first isolated in 1898 by Nocard and Roux (38). The M. myco...

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“…pneumoniae 16s rRNA sequence has approximately 70% homology to that of E. cofi (Brosius et af., 1978), a value which corresponds to those found for M . capricofum (Iwami et af., 1984) and Mycoplasma strain PG 50 (Frydenberg & Christiansen, 1985). Alignment of the 16s rRNA sequences of M .…”

Section: Results a N D Discussionmentioning

confidence: 99%

“…The following species were compared : Mycoplasma capricolum (Iwami et al, 1984), Mycoplasma strain PG 50 (Frydenberg & Christiansen, 1985), E. coli (Brosius et al, 1978), Proteus vulgaris (Carbon et al, 1981), Bacillus brevis, Bacillus stearothermophilus and Bacillus subtilis (Woese et al, 1983;Huysmans & De Wachter, 1986), and Xenopus laevis (Salim & Maden, 1981). The sequences correspond to the variable regions V3 (base 998-1043, Fig.…”

Section: U B Gobel a G E I S E R A N D E J S T A N B R I Dmentioning

confidence: 99%

Oligonucleotide Probes Complementary to Variable Regions of Ribosomal RNA Discriminate between Mycoplasma Species

Göbel

Geiser²,

Stanbridge³

1987

Microbiology

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On the basis of information from computer-assisted sequence comparison of the Mycoplasma pneumoniae 16s ribosomal RNA (rRNA) sequences with sequences from various other mycoplasmal and bacterial species, we constructed M. pneumoniae-specific oligonucleotide probes complementary to variable regions in the 16s rRNA molecule. Using a DNA/RNA dot blot hybridization procedure, it was possible to detect less than 1 x lo3 mycoplasmas. This test is a most sensitive assay for species-specific detection of bacteria. It can easily be adapted for detection and identification of other bacterial species and may have wide medical and industrial application.

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The Sequence of 16S rRNA FromMycoplasmaStrain PG50

Cited by 31 publications

References 27 publications

Genome maps of Campylobacter jejuni and Campylobacter coli

Genome maps of Campylobacter jejuni and Campylobacter coli

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

Oligonucleotide Probes Complementary to Variable Regions of Ribosomal RNA Discriminate between Mycoplasma Species

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