The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome

Chen, Xinwen; IJkel, W.F.J.; Tarchini, Renato; Sun, Xiulian; Sandbrink, H.; Wang, Hualin; Peters, Sander; Zuidema, D.; Lankhorst, René Klein; Vlak, Just M.; Hú, Zhìhóng

doi:10.1099/0022-1317-82-1-241

Cited by 200 publications

(130 citation statements)

References 59 publications

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“…The vp39 gene of the Heliothis armigera nuclear polyhedrosis virus (HaNPV) that we isolated and identified is 882bp. It has a 54% and 40% homology with that of AcMNPV in the DNA and amino acid sequences, respectively (Lorene et al, 1996;Chen et al, 2001). The objective of the current study is to corroborate the function of the HaNPV capsid protein VP39 in virus infection.…”

Section: Introductionsupporting

confidence: 60%

Interaction of Heliothis armigera Nuclear Polyhedrosis Viral Capsid Protein with its Host Actin

Lu¹,

Qi²,

Gao³

2002

BMB Reports

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In order to find the cellular interaction factors of the Heliothis armigera nuclear polyhedrosis virus capsid protein VP39, a Heliothis armigera cell cDNA library was constructed. Then VP39 was used as bait. The host actin gene was isolated from the cDNA library with the yeast two-hybrid system. This demonstrated that VP39 could interact with its host actin in yeast. In order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pEGFP39. The fusion protein was expressed in the Hz-AM1 cells under the control of the Autographa californica multiple nucleopolyhedrovirus immediate early gene promoter. The host actin was labeled specifically by the red fluorescence substance, tetramethy rhodamine isothicyanete-phalloidin. Observation under a fluorescence microscopy showed that VP39, which was indicated by green fluorescence, began to appear in the cells 6 h after being transfected with pEGFP39. Red actin cables were also formed in the cytoplasm at the same time. Actin was aggregated in the nucleus 9 h after the transfection. The green and red fluorescence always appeared in the same location of the cells, which demonstrated that VP39 could combine with the host actin. Such a combination would result in the actin skeleton rearrangement.

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Section: Introductionsupporting

confidence: 60%

Interaction of Heliothis armigera Nuclear Polyhedrosis Viral Capsid Protein with its Host Actin

Lu¹,

Qi²,

Gao³

2002

BMB Reports

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“…Therefore, at least 10 conserved baculovirus proteins are shared by ODVs of AcMNPV, CuniNPV, and HearNPV, including P49, ODV-EC27, ODV-E56, P74, GP41, VP39, P33, P6.9, ODV-EC43, and PIF-2. Another protein conserved in baculoviruses, PIF1, was identified as an ODV component in HearNPV in our study and was also reported as an ODV-associated protein in CuniNPV (46) but was not identified by multiple approaches in AcMNPV (13). DNA polymerase and helicase are conserved baculovirus proteins shared by the ODVs of both AcMNPV and HearNPV, but they were not detected in the CuniNPV ODV (46).…”

Section: Discussionmentioning

confidence: 80%

“…A culture of H. armigera insects was maintained as described by Sun et al (55). An in vivo-cloned strain of HearNPV (HearNPV-G4) (13,55) was used as the wild-type virus and propagated in H. armigera. Cells of the Heliothis zea HzAM1 line (39) were used for producing BV of HearNPV.…”

Section: Methodsmentioning

confidence: 99%

“…Phylogenetic analysis indicated that HearNPV belongs to the group II NPVs (12,29). Its DNA genome is 131 kb and contains 135 open reading frames (ORFs) that potentially encode proteins of 50 amino acids (aa) or larger (13). Several HearNPV genes, such as the polyhedrin gene (polh) (14), the ecdysteroid UDP-glucosyltransferase gene (egt) (15), the late expression factor 2 gene (lef-2) (12), the basic DNA-binding protein gene (p6.9) (61), ha122 (37), Ha94 (19), chitinase (60), fp25K (67), p10 (18), and the F-protein gene (ha133) (36), have been characterized.…”

mentioning

confidence: 99%

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Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100

Deng

Wang

Mao

et al. 2007

J Virol

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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry were used to analyze the structural proteins of the occlusion-derived virus (ODV) of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV), a group II NPV. Twenty-three structural proteins of HearNPV ODV were identified, 21 of which have been reported previously as structural proteins or ODV-associated proteins in other baculoviruses. These include polyhedrin, P78/83, P49, ODV-E18, ODV-EC27, ODV-E56, P74, LEF-3, HA66 (AC66), DNA polymerase, GP41, VP39, P33, ODV-E25, helicase, P6.9, ODV/BV-C42, VP80, ODV-EC43, ODV-E66, and PIF-1. Two proteins encoded by HearNPV ORF44 (ha44) and ORF100 (ha100) were discovered as ODV-associated proteins for the first time. ha44 encodes a protein of 378 aa with a predicted mass of 42.8 kDa. ha100 encodes a protein of 510 aa with a predicted mass of 58.1 kDa and is a homologue of the gene for poly(ADP-ribose) glycohydrolase (parg). Western blot analysis and immunoelectron microscopy confirmed that HA44 is associated with the nucleocapsid and HA100 is associated with both the nucleocapsid and the envelope of HearNPV ODV. HA44 is conserved in group II NPVs and granuloviruses but does not exist in group I NPVs, while HA100 is conserved only in group II NPVs.

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“…By using three independent approaches, we found that the concerted action of three AcMNPV genes, p26, p10 and p74, is required for proper virion occlusion in the polyhedra of AcMNPV. The three genes were found in a cluster in AcMNPV (Ayres et al, 1994) and several other NPVs, including Helicoverpa armigera single nucleocapsid NPV (Chen et al, 2001), Spodoptera exigua multiple NPV (IJkel et al, 1999), Bombyx mori NPV (Gomi et al, 1999) and Choristoneura fumiferana defective NPV (Lauzon et al, 2005). Baculoviruses replicating in the nuclei of insect cells synthesize large amounts of polyhedrin and P10.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a virion occlusion-defective Autographa californica multiple nucleopolyhedrovirus mutant lacking the p26, p10 and p74 genes

Wang

Salem

Campbell

et al. 2009

Journal of General Virology

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Nucleopolyhedroviruses (NPVs), family Baculoviridae, are insect-specific viruses with the potential to control insect pests in agriculture and forestry. NPVs are occluded in polyhedral occlusion bodies. Polyhedra protect virions from inactivation in the environment as well as assisting virions in horizontal transmission in the insect population. The process of virion occlusion in the polyhedra is undefined and the genes that regulate the virion occlusion process have not been well investigated yet. An Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutant (AcDef) that has a 2136 bp DNA deletion, including p26, p10 and p74 genes, has been isolated. No virions were detected in the polyhedra of AcDef. Restoration of all the missing sequences into AcDef led to proper virion occlusion. Individual gene deletion of either p10 or p26 could not abolish virion occlusion in the polyhedra of AcMNPV, but p10 deletion reduced virion occlusion efficiency more than threefold compared with the wild-type AcMNPV. Previous studies by other research groups on deletion of AcMNPV gene p74 suggested that p74 is a per os infectivity factor, and deletion of the p74 gene did not eliminate virion occlusion. Collectively, the three genes (p26, p10 and p74) may act in concert to regulate the virion occlusion process. Therefore, p26, p10 and p74 are all required for proper virion occlusion in the polyhedra of AcMNPV.

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The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome

Cited by 200 publications

References 59 publications

Interaction of Heliothis armigera Nuclear Polyhedrosis Viral Capsid Protein with its Host Actin

Interaction of Heliothis armigera Nuclear Polyhedrosis Viral Capsid Protein with its Host Actin

Proteomics Analysis of Helicoverpa armigera Single Nucleocapsid Nucleopolyhedrovirus Identified Two New Occlusion-Derived Virus-Associated Proteins, HA44 and HA100

Characterization of a virion occlusion-defective Autographa californica multiple nucleopolyhedrovirus mutant lacking the p26, p10 and p74 genes

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