The sequential changes in DNA synthesis, glucose utilization, protein synthesis, and peripheral benzodiazepine receptor density in C6 brain tumors after chemotherapy to predict the response of tumors to chemotherapy

Takeda, Norio; Dikšić, Mirko; Yamamoto, Y.

doi:10.1002/(sici)1097-0142(19960315)77:6<1167::aid-cncr25>3.0.co;2-z

Cited by 12 publications

(6 citation statements)

References 49 publications

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“…5) was similar to that observed ex vivo after tissue excision (22.4% 6 5.4% and 31.7% 6 12.7%; Table 1). Our study on doxorubicin, cisplatin, and 5-fluorouracil treatment of C6 cells grown in vitro (14) and the studies of other researchers on animal models (27,28) suggest that this early decline of 18 F-FDG uptake in treated tumors indicates a reduction of the viable cell fraction within the tumor. Larger variance in the biodistribution data (Table 1) than in the PET scans (Fig.…”

Section: Discussionmentioning

confidence: 58%

Rapid Reduction of 1-Receptor Binding and 18F-FDG Uptake in Rat Gliomas After In Vivo Treatment with Doxorubicin

Waarde¹,

Shiba²,

Jong³

et al. 2007

Journal of Nuclear Medicine

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Section: Discussionmentioning

confidence: 58%

Rapid Reduction of 1-Receptor Binding and 18F-FDG Uptake in Rat Gliomas After In Vivo Treatment with Doxorubicin

Waarde¹,

Shiba²,

Jong³

et al. 2007

Journal of Nuclear Medicine

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“…The brains were stored at −84°C in a sealed, freezer bag until their use. The brains were sectioned in 20 μm thick slices in a microtome-cryostat at −25°C and placed on a gelatin coated microscopic glass as previously described (Palacios et al 1988; Takeda et al 1996). In short, the slices were mounted on the gelatin-coated microscope slides and stored at room temperature overnight and then stored in slide boxes with desiccant (at least 2 days) at −20°C until use.…”

Section: Methodsmentioning

confidence: 99%

A genetic rat model of depression, Flinders sensitive line, has a lower density of 5-HT1A receptors, but a higher density of 5-HT1B receptors, compared to control rats

Nishi

Kanemaru

Dikšić

2009

Neurochemistry International

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Deficiencies in brain serotonergic neurotransmission, which is in part associated with the alteration of brain serotonin (5-HT) receptors, have been proposed as part of a neurochemical imbalance in affective disorders, including depression. The drugs used for the treatment of these disorders generally act through and/or on the serotonergic system. Different animal models of depression have provided researchers with tools to obtain a better understanding of drug actions and possibilities to obtain insight into the neurochemical bases of these disorders. The measurements of the 5-HT 1A and 5-HT 1B receptor densities in a rat model of depression, Flinders Sensitive Line (FSL) rats, and comparisons with Sprague-Dawley (SPD) and Flinders Resistant Line (FRL) rats, are reported here. The receptor sites were quantified by autoradiography in more than twenty-five distinct brain regions known to have relatively large densities of respective sites. Some brain regions (e.g., dental gyrus, septal nucleus) were divided into several parts, according to previously known subdivisions, because of a substantial heterogeneity of these receptors. The densities in the FSL rats ("depressed" rats) were compared statistically to those in the SPD rats. In addition, comparisons were made to the densities in the FRL rats (rats not showing depressive symptoms). Comparisons were performed with the SPD and FRL rats because both of these strains have been used as control animals in studies of FSL rats. The results show that the densities of 5-HT 1A receptors are not significantly different between the FSL and SPD rats, but they are significantly different from the FRL rats. 5-HT 1A receptor density is significantly higher in the FRL rats than the SPD rats. The 5-HT 1B receptors were significantly greater in the FSL rats than in either the SPD or FRL rats. In addition, the FRL rats have 5-HT 1B receptor densities significantly lower in many brain regions than the SPD rats. The data presented here, in addition to previously reported differences in regional synthesis between these strains and the effect of acute citalopram on synthesis, suggest that SPD rats are likely a more appropriate control than FRL rats, when studies of FSL rats are performed with drugs acting directly or indirectly on, or through, the brain serotonergic system. However, comparisons, particularly of neurochemical and/ or biological parameters in FRL rats, may reveal new insight into the alterations of 5-HT neurotransmission in this animal model of depression and possibly human depression, as well as the elevation of symptoms with treatments. The data also suggest that there could be a different fraction 3Correspondence to:

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“…For example, the correct choice of chemotherapeutic agents or the fractionation schedule for radiation therapy treatments frequently depends on the proliferative status of a tumour (Schabel, 1969;Thames et al, 1983;Begg et al, 1992;Corro et al, 1995, Takeda et al, 1996. It is well known that slowly proliferating tumours respond better to cell cycle non-specific agents and/or conventional radiation therapy schedules, while rapidly proliferating tumours respond better to cell cycle specific agents and/or hyperfractionated radiation therapy schedules.…”

mentioning

confidence: 99%

Sigma-2 receptors as a biomarker of proliferation in solid tumours

et al. 2000

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Over the past several years, our group has provided considerable evidence that the expression of sigma-2 (σ2) receptors may serve as a biomarker of tumour cell proliferation. In these in vitro studies, σ2receptors were expressed 8–10 times more in proliferative (P) tumour cells than in quiescent (Q) tumour cells, and the extent and kinetics of their expression were independent of a number of biological, physiological and environmental factors often found in solid tumours. Moreover, the expression of σ2receptors followed both the population growth kinetics when Q-cells were recruited into the P-cell compartment and the proliferative status of human breast tumour cells treated with cytostatic concentrations of tamoxifen. However, these in vitro studies may or may not be indicative of what might occur in solid tumours. In the present study, the σ2receptor P:Q ratio was determined for the cells from subcutaneous 66 (diploid) and 67 (aneuploid) tumours grown in female nude mice. The σ2receptor P:Q ratio of the 66 tumours was 10.6 compared to the σ2receptor P:Q ratio of 9.5 measured for the 66 tissue culture model. The σ2receptor P:Q ratio of the 67 tumours was 4.5 compared to the σ2receptor P:Q ratio of ≈ 8 measured for the 67 tissue culture model. The agreement between the solid tumour and tissue culture data indicates that: (1) the expression of σ2receptors may be a reliable biomarker of the proliferative status of solid tumours and (2) radioligands with both high affinity and high selectivity for σ2receptors may have the potential to non-invasively assess the proliferative status of human solid tumours using imaging techniques such as positron emission tomography or single-photon emission computerized tomography. © 2000 Cancer Research Campaign

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The sequential changes in DNA synthesis, glucose utilization, protein synthesis, and peripheral benzodiazepine receptor density in C6 brain tumors after chemotherapy to predict the response of tumors to chemotherapy

Cited by 12 publications

References 49 publications

Rapid Reduction of 1-Receptor Binding and 18F-FDG Uptake in Rat Gliomas After In Vivo Treatment with Doxorubicin

Rapid Reduction of 1-Receptor Binding and 18F-FDG Uptake in Rat Gliomas After In Vivo Treatment with Doxorubicin

A genetic rat model of depression, Flinders sensitive line, has a lower density of 5-HT1A receptors, but a higher density of 5-HT1B receptors, compared to control rats

Sigma-2 receptors as a biomarker of proliferation in solid tumours

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