The Serological Analysis of the a Blood Group Substance(s)

Borel, J. F.

doi:10.1007/978-94-017-6289-2_68

Cited by 2 publications

(1 citation statement)

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“…Antigens cross-reactive with the A antigen of human red cells have been demonstrated on the red cells of several subhuman species, notably cattle, sheep and pigs [1,2,91. Despite early work by VON DUNGERN and HIRSZFELD [15] suggesting that canine red cells might be distinguished by their reactions with human typing sera, definitive demonstration of similar antigens on canine red cells has been lacking.…”

Section: Discussionmentioning

confidence: 99%

Tr: A Canine Red Cell Antigen Related to the A‐antigen of Human Red Cells

et al. 1971

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A canine red cell antigen, Tr, was defined by means of an immune canine antiserum produced by isoimmunization of a dog with canine red cells. The specificity of the antiserum was found t o be identical t o that of a naturally-occurring canine antiserum which reacted specifically with human group A red cells after absorption with human group 0 and B red cells. Absorption and agglutinationinhibition studies with human saliva showed that the canine red cell antigen Tr is related to the A antigen of human red cells; its representation on the canine red cell appears to be weaker than that of the A antigen of human A, red cells. The incidence of the Tr antigen on canine red cells is approximately 50%. No relationships were established between the canine A, B, C and D blood types and the ABH system of man.Studies with isoimmune antisera have defined a series of red cell typing systems in the domestic dog. The designations A, B, C, D, E, F and G applied to these systems do not imply relationships to similarly named blood types in other species, and systematic attempts t o establish such relationships have not been made [13, 141. In other subhuman species, including cattle, sheep and pigs, red cell antigens cross-reactive with the A antigen of human red cells have been demonstrated [I, 2, 91. The present study defines a canine red cell antigen, Tr, cross-reactive with the human A antigen, and unrelated t o the antigens of the conventional canine typing systems. Materials and MethodsHuman and canine red cells collected by venepuncture were washed 3 times and suspended in 0.15 M NaCl for agglutination tests, using 2% suspensions for canine cells and 4% for human. In some instances, human cells were stored as whole blood in Alsever's solution for 1 to 7 days before use.Canine typing sera were prepared by isoimmunization of dogs by the small volume method of SWISHER, YOUNG and TRABOLD 114.1, modified by the use of packed, washed red cells instead of whole blood. Antisera were compared for specificity with standard antiseras. Human saliva from normal secretor subjects of blood groups 0, A,, and B was centrifuged at 10,000 rpm for 30 min, filtered (Millipore 0.22 ,u m.p.), heated in a boiling waterbath for 10 min and stored a t -20°C. Selected salivas were (a) 0 saliva, 1 in 10 dilution, (b) A, saliva, 1 in 5 dilution, which reduced the titer of an AJanti-A reaction from 64 t o 4 and (c) B saliva, 1 in 20 dilution, which reduced a human B/anti-B titer from 32 to 4. Agglutination reactions were performed as (a) single tube tests with 0.1 ml of serum and 0.1 ml red cell suspension, scored macroscopically after centrifuging from 0 to + + + +, and (b) classical twofold dilution titrations by the method of FOERSTER [3]. Inhibition studies employed 0.1 ml vol of diluted saliva, incubated with the antiserum for 15 min prior to the addition of red cells. Incubation conditions were 4°C for 60 min for all reactions with the exception of those utilizing canine anti-A serum, when 37°C for 15 min was used.Absorption studies employed equal v...

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Section: Discussionmentioning

confidence: 99%

Tr: A Canine Red Cell Antigen Related to the A‐antigen of Human Red Cells

et al. 1971

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The reactivity of canine red cells with heterophile agglutinins having anti‐A activity*

Bowdler

Uhlenbruck²,

Dries

et al. 1973

Animal Blood Groups and Biochemical Genetics

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SummaryHeterophile A‐reactive agglutinins, obtained from the seeds of Dolichos biflorus and from the snails Helix aspersa, Helix pomatia and Cepaea nemoralis, were tested for reactivity with the saliva and red cells of a random series of dogs. Saliva from dogs with the A‐like Tr red cell antigen inhibited the agglutination of human A red cells by each of the reagents. However, none of the heterophile agglutinins distinguished Tr+ from Tr— red cells: the Dolichos agglutinin was non‐reactive, while all of the snail reagents agglutinated both Tr+ and Tr— cells. It is concluded that the canine red cell bears a series of heterophile receptors which mask reactivity with the canine homologue of the red cell A‐antigen, and that the restricted size of the binding sites of the agglutinins limits the informational significance of cross‐reactivity obtained with these reagents.

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The Serological Analysis of the a Blood Group Substance(s)

Cited by 2 publications

References 20 publications

Tr: A Canine Red Cell Antigen Related to the A‐antigen of Human Red Cells

Tr: A Canine Red Cell Antigen Related to the A‐antigen of Human Red Cells

The reactivity of canine red cells with heterophile agglutinins having anti‐A activity*

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