The serological diagnosis of cysticercosis

Proctor, E. M.; Powell, Steve; Elsdon‐Dew, R.

doi:10.1080/00034983.1966.11686398

Cited by 34 publications

(7 citation statements)

References 14 publications

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“…Serological cross-reaction between the three larval cestode species of E. granulosus, T. hydatigena and T. ovis found in sheep has been reported previously3) (II) (16) (19). In those studies, positive reactions were observed in all sera, regardless of whether the antigen from one species was used to test the sera from sheep infected with the homologous or heterologous species.…”

Section: Discussionmentioning

confidence: 82%

Echinococcus granulosus, Taenia hydatigena, T. ovis:Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep

Yong

Heath

Parmeter

1978

New Zealand Veterinary Journal

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In order to monitor the progress of New Zealand's hydatids eradication campaign, a specific, serological, diagnostic test is required to identify infected sheep. An indirect haemagglutination test, using pyruvic aldehyde-stabilized sheep erythrocytes as the antigen carrier, was developed for the serodiagnosis oflarval cestode infections of sheep. Using cyst fluid from Echinococcus granulosus, Taenia hydatigena and T. ovis as the antigens in this test, it was shown that the larval cestode species, responsible for an infection in sheep harbouring a single specific infection, could be identified by the higher titre given with the homologous antigen, in comparison to that given with the heterologous antigens. Sera of sheep infected with two or more species were also tested by this method, and the only specific infections to be diagnosed by differential titres were those due to the presence of live E. granulosus cysts. These antigens cannot be used for the diagnosis of specific larval cestode infections in the field because of the cross-reactivity between cyst fluids. However, the test did show that infection with larval cestodes could be diagnosed on a non-specific basis.

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Section: Discussionmentioning

confidence: 82%

Echinococcus granulosus, Taenia hydatigena, T. ovis:Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep

Yong

Heath

Parmeter

1978

New Zealand Veterinary Journal

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“…As reações de precipitação em tubos de gel de agar possibilitam diferenciar os sistemas antigênicos envolvidos, podendo também quantificar suas concentrações, mas sua utilização ainda é limitada, pois são pouco sensíveis e praticamente nenhum sucesso foi obtido no seu emprego como auxílio diagnóstico em LCR 4, 6,13,18,34 . A reação de hemaglutinação (RHA) é simples e rápida, entretanto para poder comparar os resultados entre laboratórios é necessário que se uniformize o método de preparo dos antígenos empregados pois, apesar de ser mais sensível que a RFC, ainda mostra inespecificidade elevada 5,14,17,25,26,27,29,36 . Diferentes antígenos foram empregados para a padronização da reação do imunofluorescência (RIF) ao diagnóstico da cisticercose humana 10,11,15,23,35,38 , a aplicabilidade dessa reação foi comprovada nos estudos de Bassi e coI.…”

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Teste imunoenzimático (Elisa) no diagnóstico da neurocisticercose: estudo de diferentes extratos antigênicos na detecção de anticorpos IgG em amostras de soro e de líquido cefalorraqueano

Costa

1986

Arq. Neuro-Psiquiatr.

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Five different antigens from Cysticercus cellulosae, a vesicular fluid, a saline and an alkaline total extracts, an escolex and membrane, were studied in the ELISA immunoenzymatic assay to demonstrate IgG antibodies in cerebrospinal fluid (CSF) and serum samples. For the 5 antigens the 20 micrograms/ml concentration was selected for polyvinyl plates sensitization for CSF and serum assays. The IgG fraction of a sheep anti-human IgG antiserum was labeled with horseradish peroxidase and revealed with hydrogen peroxide-5-aminosalicilic acid. For positive results 10 ELISA Units for sera and 5 EU for CSF were taken. A total of 182 serum samples and 115 CSF samples were tested. The ELISA sensitivity for sera were 88.6% for vesicular fluid, escolex and membrane; 85.7% for saline extract and 62.8% for alkaline extract. The ELISA sensitivity for CSF was 100% for vesicular fluid and saline total extract, 91.7% for membrane, 89.6% for excolex and 77.1% for alkaline. The ELISA specificity for sera was 100% to the 5 antigens studied; to the CSF was 100% for the alkaline and escolex and 98.5% for the other antigens. An ELISA geometric mean titer of sera and CSF was respectively the 121.1 and 27.3 for vesicular fluid, 111.6 and 31.1 for saline total extract, 30.3 and 5.3 for alkaline total extract, 63.2 and 14.8 for escolex and 69.1 and 18.8 for membrane antigen. ELISA was then compared to immunofluorescence, hemagglutination and complement fixation tests in CSF and sera. ELISA immunoenzymatic assay with saline total extract is recommended for the easy preparation and for the high quantity of antigens obtained, for the high sensitivity and great specificity for sera and CSF; we suggest that this test may be used as a substitute for immunofluorescence, hemagglutination and complement fixation in sera or CSF for the diagnosis of neurocysticercosis.

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“…On the other hand, crude extract of total cysticerci, soluble extracts from parts of cysticerci (membrane or scolex), crude extract of Taenia solium, or semipuri®ed proteins have been used as antigens by many researchers (Biagi et al 1961;Powell et al 1966;Proctor et al 1966;Flisser et al 1980;Nascimento and Mayrink 1984;Nascimento et al 1987a;Tsang et al 1989).…”

Section: Discussionmentioning

confidence: 99%

Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis

Maia

Pianetti

et al. 1999

Parasitology Research

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Human neurocysticercosis, due to infection of the central nervous system by cysticerci of Taenia solium, is a severe form of neurologic disease occurring in Central and South America. Specific proteins from scolex antigen from cysticerci were purified by polyacrylamide gel electrophoresis and electroelution and recognized in Western blots by antibodies present in sera from patients with neurocysticercosis. The proteins appeared as 13-, 17-, and 26-kDa bands on Coomassie blue-stained gels and proved to be specific to cysticerci of T. solium. No cross-reactivity with sera from patients with taeniasis or hydatidosis was observed. Enzyme-linked immunosorbent assay using the purified proteins of 13, 17, and 26 kDa demonstrated rates of 53%, 88%, and 100% specificity, respectively, at the cutoff serum dilution of 1:32 for the specific immunodiagnosis of human neurocysticercosis.

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The serological diagnosis of cysticercosis

Cited by 34 publications

References 14 publications

Echinococcus granulosus, Taenia hydatigena, T. ovis:Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep

Echinococcus granulosus, Taenia hydatigena, T. ovis:Evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep

Teste imunoenzimático (Elisa) no diagnóstico da neurocisticercose: estudo de diferentes extratos antigênicos na detecção de anticorpos IgG em amostras de soro e de líquido cefalorraqueano

Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis

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