The Serological Heterogeneity of Pseudomonas syringae pv. atrofaciens Strains and Their Ecological Niches

Пасічник, Л. А.; Yakovleva, Lyudmila; Gvozdyak, R. I.; Vassilev, Vassil

doi:10.1023/b:mici.0000008377.53112.ae

Cited by 3 publications

(12 citation statements)

References 8 publications

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“…Штами, виділені з бур'янів лободи білої, хвощу польового, енотери дворічної, грициків звичайних, осоту польового та будяка польового, були однорідні за антигенним складом і належали до серогрупи ІV. [18].…”

Section: таблиця 1 результати подвійної дифузії в агарі штамів із пшеunclassified

“…При аналізі штамів Р. syringae рv. atrofaciens із жита в Україні додатково виявлено бактерії, які віднесено до серогрупи І [18], яка відповідає серогрупі PERSAVTOM2 за класифікацією M. Saunier та ін. [7].…”

Section: таблиця 1 результати подвійної дифузії в агарі штамів із пшеunclassified

“…Відомо, що за набором серологічно активних детермінант штами різних патоварів можуть відрізнятися [5,7,17]. Тому для більш чіткого уявлення про антигенний склад і приналежність штамів бактерій до певних серологічних груп здійснювали їх серогрупування за використання реакції ПДА (табл.1).…”

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Serological Features of Bacteria Pseudomonas syringae Agroecosystems of Cereal

Pasichnyk¹,

Butsenko²

2018

Mikrobiol. Z.

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Section: таблиця 1 результати подвійної дифузії в агарі штамів із пшеunclassified

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Serological Features of Bacteria Pseudomonas syringae Agroecosystems of Cereal

Pasichnyk¹,

Butsenko²

2018

Mikrobiol. Z.

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“…The dominance of strains of these serogroups is also characteristic for P. syringae, isolated from wheat [11,13], which indicates the prevalence of the serogroup not only in the bacterial phytopathogens isolated from cereals, but also in other plants of agrophytocenosis such as weeds.…”

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“…The strains of serogroup V are rarely met among bacteria isolated from wheat [13], as well as the strains of P. syringae, isolated from weeds were also presented in this serogroup in limited quantities. P. syringae strain serogroup I was found for the first time on weeds in wheat agrophytocenosis.…”

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The Spectrum of Weed Phytopathogens in Wheat Agrophytocenosis

Pasichnyk¹

2016

Mikrobiol. Z.

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Bacterial diseases, pests and weeds are the main factors that do not allow generating high yields of agricultural crops. The article presents the results of a study of agents of weed diseases. It was shown that the affected weeds in wheat agrophytocenosis present an ecological niche for phytopathogenic bacteria, belonging to the species Pseudomonas syringae, Pseudomonas viridiflava, Pseudomonas fluorescens, Pectobacterium carotovorum, Pantoea agglomerans, Xanthomonas translucens and Pseudomonas sp. In addition to these pathogens, phytopathogenic yeasts Rhodosporidium diobovatum and Rhodotorula sp. were isolated and identified. Most of the phytopathogens, isolated from weeds, are widely specialized and in experiment affect various species of weeds and cereals. Fatty acid composition of the total cellular lipids of these plant pathogens was identified and proved to be characteristic for each species of bacteria. P. syringae was determined as the main agent of bacterial diseases of weeds, its strains have a high degree of serological relationship to the agent of basal bacteriosis of cereals -P. syringae pv. atrofaciens and belong to the same serogroups. The results of RAPD-PCR analysis revealed that P. syringae strains isolated from weeds and disease agents of cereals present a genetically homogeneous group. The results obtained indicate that wheat and weeds are affected by the same agents of bacterial diseases. K e y w o r d s: phytopathogenic bacteria, agent, bacterial disease, weeds, fatty acids, serology.

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Modernization of the Pseudomonas syringae Pathovars Serogrouping Scheme

Pasichnyk¹,

Butsenko²

2022

Mikrobiol. Z.

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In 1979, L.T. Pastushenko and I.D. Symonovych developed a scheme of serogrouping phytopathogenic bacteria of the Pseudomonas genus, which is still used now. However, today’s using this serogrouping scheme is complicated by the lack of all data accumulated over the years of its application. Moreover, the scheme does not correspond to the modern taxonomy of phytopathogenic bacteria of the Pseudomonas genus. Aim. On the basis of own experimental results and data of scientific literature, to carry out modernization of the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus. Methods. The strains of Pseudomonas syringae pathovars such as atrofaciens, coronafaciens, tabaci, which were isolated from plants of wheat, rye, oats, tobacco, and various species of affected weeds in different regions of Ukraine have been studied in the work. Antigenic properties of bacterial strains were studied by agglutination and precipitation reactions (the Ouchterlony double immunodiffusion techniques) using antisera to P. syringae strains of nine serological groups (I, II, III, IV, V, VI, VII, VIII, IX). To carry out the precipitation reaction, O- and OH-antigens were obtained by a modified Grasse’s method. The presence of the same number of precipitation lines of the studied antigens as the number of lines with homologous antiserum of the corresponding serogroup testified to their belonging to this serogroup according to the known serogrouping scheme of phytopathogenic bacteria developed in 1979 by L.T. Pastushenko and I.D. Symonovych. Results. It has been proved that strains of P. syringae pathovars isolated from different cereals (rye, wheat, oats) and segetal vegetation differ in antigenic composition. The antigenic composition of P. syringae strains depends on the host plant from which the pathogen was isolated. Strains of the causative agent of basal glume rot P. syringae pv. atrofaciens isolated from wheat belong to four serological groups (II, IV, V, VI), from rye — to five serological groups (I, II, IV, V, VI), as well as strains of this pathogen isolated from segetal vegetation of wheat agrophytocenosis. Strains of the halo bligth P. syringae pv. coronafaciens from affected oat plants belong to two serological groups (I, V). The serogrouping scheme has been supplemented by new data on the antigenic properties of P. syringae pv. tomato, the causative agent of the bacterial speck disease of tomatoes (Solanum lycopersicum), which is classified as serogroup IV. It has been found that P. syringae pv. tabaci strains, which cause wildfire of tobacco, are part of three serogroups — VII, VIII, IX, and not of two ones, as was presented in the known scheme (1979). Conclusions. Therefore, on the basis of our own research and literature data, the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus developed by L.T. Pastushenko and I.D. Symonovych has been modernized. In the renovated scheme, current species’ names of phytopathogens are present, this scheme introduces new information about the serogroups of P. syringae pv. atrofaciens isolated from rye seeds and plants, weeds, P. syringae pv. coronafaciens — from oat plants, P. syringae pv. tabaci — from tobacco, and P. syringae pv. tomato — from affected tomato plants.

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The Serological Heterogeneity of Pseudomonas syringae pv. atrofaciens Strains and Their Ecological Niches

Cited by 3 publications

References 8 publications

Serological Features of Bacteria Pseudomonas syringae Agroecosystems of Cereal

Serological Features of Bacteria Pseudomonas syringae Agroecosystems of Cereal

The Spectrum of Weed Phytopathogens in Wheat Agrophytocenosis

Modernization of the Pseudomonas syringae Pathovars Serogrouping Scheme

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