The Serology Diagnostic Schemes in Borrelia burgdorferi Sensu Lato Infections – Significance in Clinical Practice

Tokarska-Rodak, Małgorzata; Kozioł-Montewka, Maria

doi:10.5772/34150

Cited by 2 publications

(6 citation statements)

References 32 publications

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“…OspC, OspA, and p41 are considered the most immunogenic proteins of B. burgdorferi 19,20 ; consistently, in this study IgGs targeting these antigens were also the most frequent and they reached the highest levels (Fig. 1).…”

Section: Discussionsupporting

confidence: 80%

“…1). Other important targets for IgG diagnostics include VisE, p83, p58, and p17 19,20 , also detected in this study. Interestingly, in many patients we observed antibodies targeting different species (e.g.…”

Section: Discussionsupporting

confidence: 63%

“…Serological identi cation of SARS-SoV-2 infection was conducted with: Microblot -Array COVID-19 IgG (TestLine Clinical Diagnostics s.r.o. ); the array contains selected fragments of speci c NCP, RBD and Spike S2 antigens of SARS-CoV-2 virus.…”

mentioning

confidence: 99%

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Correlation between COVID-19 severity and previous exposure of patients to Borrelia spp.

Szewczyk-Dąbrowska

Budziar

Harhala

et al. 2022

Preprint

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Predictors for the risk of severe COVID-19 are crucial for patient care and control of the disease. Other infectious diseases as potential comorbidities in SARS-CoV-2 infection are still poorly understood. Here we identify association between the course of COVID-19 and Lyme disease (borreliosis), caused by Borrelia burgdorferi transmitted to humans by ticks. Exposure to Borrelia was identified by multi-antigenic (19 antigens) serological testing of patients: severe COVID-19 (hospitalized), asymptomatic to mild COVID-19 (home treated or not aware of being infected), and not infected with SARS-CoV-2. Increased levels of Borrelia-specific IgGs strongly correlated with COVID-19 severity and risk of hospitalization. This suggests that a history of tick bites and related infections may contribute to the risks in COVID-19. Though mechanisms of this link is not clear yet, screening for antibodies targeting Borrelia may help accurately assess the odds of hospitalization for SARS-CoV-2 infected patients, supporting efforts for efficient control of COVID-19.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 63%

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Correlation between COVID-19 severity and previous exposure of patients to Borrelia spp.

Szewczyk-Dąbrowska

Budziar

Harhala

et al. 2022

Preprint

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“…As long as the infection with B . burgdorferi progresses, the immunological response may expand to an increasing number of proteins, including p21 (81·3% of our samples), p58 (75% of the cases), p19 (50% of our samples) and p83 (25% of our samples) (Tokarska‐Rodak & Kozio‐Montewk, ), all of which were observed during the Western blot confirmation.…”

Section: Discussionmentioning

confidence: 75%

“…We found a variable band profile in the positive samples during the Western blot confirmation. The most frequently detected antigen was p41 (in 100% of the cases), which is most commonly recognised in patients with Lyme disease and is highly immunogenic (Luft et al ., ; Tokarska‐Rodak & Kozio‐Montewk, ). Generally, antibodies to p41 appear in the early phase of infection but can also be observed during the late stages.…”

Section: Discussionmentioning

confidence: 99%

Serological evidence of Borrelia circulation among blood donors in the São Paulo state, Brazil

Slavov

Christova

Ferreira

et al. 2019

Transfusion Medicine

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SUMMARY Objective The objective of this study was to examine the Borrelia seroprevalence among blood donors in Southeast Brazil. Background There is evidence that Borrelia spirochetes are circulating in Brazil; however, there are no studies that characterise these bacteria and investigate their seroprevalence in the Brazilian population. Such a situation, combined with a recent outbreak of tick‐borne Rocky Mountain spotted fever in the São Paulo state demonstrates the increasing role of ticks as arthropod vectors in Brazil. Methods For the purpose of the study, 452 blood donors from Ribeirão Preto city, São Paulo state were tested using anti‐Borrelia immunoglobulin G (IgG) assay. The positive results were also confirmed by Western blot for anti‐borrelia IgM/IgG. Results The anti‐Borrelia IgG enzyme‐linked immunosorbent assay (ELISA) showed nine positive and nine borderline reactive samples, giving a total seroprevalence of 2·0% of anti‐Borrelia IgG among Brazilian blood donors. The confirmation of the seropositive and borderline samples by Borrelia Western blot was demonstrated by IgG‐positive results in 16 samples (a seroprevalence of 3.5%). Anti‐Borrelia IgM antibodies were also detected in one sample. Conclusions Our results demonstrate that Borrelia‐like spirochetes may be circulating among blood donors from the São Paulo State and that the ticks have an important epidemiological role as vectors of bacterial infections in this Brazilian region. These results not only alert us to possible actions that might be undertaken in order to completely characterise the aetiological agents of Lyme‐like syndromes in Brazil but also the possible impact that these bacterial agents might have on haemotherapy practices.

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The Serology Diagnostic Schemes in Borrelia burgdorferi Sensu Lato Infections – Significance in Clinical Practice

Cited by 2 publications

References 32 publications

Correlation between COVID-19 severity and previous exposure of patients to Borrelia spp.

Correlation between COVID-19 severity and previous exposure of patients to Borrelia spp.

Serological evidence of Borrelia circulation among blood donors in the São Paulo state, Brazil

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