The serotonin transporter-imipramine "receptor".

Talvenheimo, Jane; Fishkes, H; Nelson, Pamlea J.; Rudnick, Gary

doi:10.1016/s0021-9258(18)32380-9

Cited by 83 publications

(29 citation statements)

References 15 publications

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“…Therefore, another aspect of the original proposal must be reevaluated, because uptake of Na + and 5-HT + coupled to K + efflux would be electrogenic, moving one positive charge into the cell with each 5-HT molecule. The 1:1 transport stoichiometry for Na + (Talvenheimo et al, 1983) and K + (Rudnick, 1977; Fig. 3) with 5-HT agrees well with the valence of the Na + -dependent peak current and its reversal by intracellular K + (Hasenhuetl et al, 2016).…”

Section: Back To the Stoichiometrysupporting

confidence: 71%

“…2 B) and plotting Δμ 5-HT , the chemical potential of the 5-HT gradient, against Δμ Na + , gave a straight line with a slope of 0.9 (Fig. 2 C), strongly suggesting a 1:1 5-HT/Na + stoichiometry (Talvenheimo et al, 1983). For K + , the transmembrane 5-HT gradient i /[5-HT] o ) was a linear function of the K + gradient ([K + ] I /[K + ] o ) suggesting 1:1 stoichiometry also for K + (Rudnick, 1977;Fig.…”

Section: Introductionmentioning

confidence: 86%

“…As stated above, 5-HT transport is electroneutral. This observation ruled out the possibility that a transmembrane K + gradient generated a diffusion potential that drove transport electrically (Rudnick, 1977) and instead favored a mechanism in which K + efflux was (Talvenheimo et al, 1983), reprinted with permission from the Journal of Biological Chemistry. (D) Accumulation of intravesicular 5-HT vs. the imposed K + gradient (both expressed as the ratio of solute in/out).…”

Section: Introductionmentioning

confidence: 99%

“…Na + and K + stoichiometry Because platelet plasma membrane vesicles could establish an equilibrium between 5-HT accumulation and the Na + and K + gradients that were driving it, we were able to measure the thermodynamic coupling between 5-HT uptake and gradients of the two ions (Rudnick, 1977;Talvenheimo et al, 1983). This coupling could be directly translated into a stoichiometry for ion-coupled 5-HT transport.…”

Section: Introductionmentioning

confidence: 99%

“…(C) Replot of data from B as the chemical potential of the accumulated 5-HT gradient vs. the chemical potential of the imposed Na + gradient. From(Talvenheimo et al, 1983), reprinted with permission from the Journal of Biological Chemistry. (D) Accumulation of intravesicular 5-HT vs. the imposed K + gradient (both expressed as the ratio of solute in/out).…”

mentioning

confidence: 99%

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Serotonin transport in the 21st century

Rudnick

Sandtner

2019

Journal of General Physiology

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Serotonin (5-hydroxytryptamine [5-HT]) is accumulated within nerve endings by the serotonin transporter (SERT), which terminates its extracellular action and provides cytoplasmic 5-HT for refilling of synaptic vesicles. SERT is the target for many antidepressant medications as well as psychostimulants such as cocaine and ecstasy (3,4-methylenedioxymethamphetamine). SERT belongs to the SLC6 family of ion-coupled transporters and is structurally related to several other transporter families. SERT was studied in the 1970s and 1980s using membrane vesicles isolated from blood platelets. These studies led to a proposed stoichiometry of transport that has been challenged by high-resolution structures of SERT and its homologues and by studies of SERT electrophysiology. Here, we review the original evidence alongside more recent structural and electrophysiological evidence. A self-consistent picture emerges with surprising insights into the ion fluxes that accompany 5-HT transport.

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Section: Back To the Stoichiometrysupporting

confidence: 71%

Section: Introductionmentioning

confidence: 86%