The serotype distribution of <i>Campylobacter jejuni</i> and 

<i>C. coli</i> isolated from patients with diarrhoea and controls at
 Tikur 

Anbassa Hospital, Addis Ababa, Ethiopia

Asrat, Daniel; Hathaway, A.; Sjögren, Eva; Ekwall, Erik; Kaijser, B

doi:10.1017/s0950268896007315

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…However, this level of typeability is comparable to that from a recent study of poultry campylobacter isolates in The Netherlands where 18% of isolates were found to be untypeable by using the Penner serotyping technique (11). The most recently published data from studies using the Penner scheme for isolates from humans have been those from studies carried out in China and Japan (17) and Ethiopia (4). The quoted untypeability rates are 58.8% for Chinese isolates, 13.0% for Japanese isolates, and 63.4% for Ethiopian isolates.…”

Section: Discussionsupporting

confidence: 79%

Serotyping Scheme for Campylobacter jejuni and Campylobacter coli Based on Direct Agglutination of Heat-Stable Antigens

et al. 1998

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Campylobacter is now the most frequently reported cause of gastrointestinal disease in England and Wales, yet few isolates are characterized beyond the genus level. The majority of isolates areCampylobacter jejuni (90%), with most of the remainder being Campylobacter coli. We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has been replaced by detection of heat-stable antigens by direct bacterial agglutination; absorbed antisera are used where appropriate. This scheme has been used to type 2,407 C. jejuni samples and 182 C. coli samples isolated in Wales between April 1996 and March 1997. Forty-seven C. jejuni serotypes were identified, with the 10 most prevalent serotypes accounting for 53% of the isolates tested; 19% of the isolates were untypeable. Only fifteenC. coli serotypes were identified, with three serotypes accounting for 69% of the isolates. This scheme provides a baseline for epidemiological studies of C. jejuni and C. coli.

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Section: Discussionsupporting

confidence: 79%

Serotyping Scheme for Campylobacter jejuni and Campylobacter coli Based on Direct Agglutination of Heat-Stable Antigens

et al. 1998

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“…Serotyping C. jejuni/coli strains from human patients in Etiopia, Asrat et al (1997), in accordance with other countries of the world, found the highest frequency of occurrence of serotypes HS 1, 3, 8, 26 and 34. These authors, however, found that about a quarter of strains was positive for more than 1 serotype.…”

Section: Discussionsupporting

confidence: 79%

“…The Penner's serotyping scheme uses 62 antisera (HS) at present, and the Lior's system works with 122 antisera (HL) (Asrat et al 1997). Regarding the high number of antisera it is clear that only a selected group of them is mostly used.…”

Section: Discussionmentioning

confidence: 99%

Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method

Steinhauserova¹,

Fojtíková²

1999

Acta Vet. Brno

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Steinhauserová I., K. Fojtíková: Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method. Acta Vet. Brno 1999, 68: 149-154. A total number of 178 strains of Campylobacter spp. from human patients suffering from diarrhea and from the intestinal content of slaughtered pigs and poultry was isolated during years 1996 to 1997. Campylobacter strains were isolated and identified by standard tests and the PCR method using fla A and fla B, as well. Isolates originating from human patients were identified as Campylobacter jejuni (98% = 108 strains) and C. coli (2% = 2 strains). Campylobacter spp. (20) strains obtained from slaughtered pigs were identified using standard tests in 45% (9 strains) and 40% (8 strains) as C. coli and C. jejuni, respectively. Strains of Campylobacter spp. (48) isolated from slaughtered poultry were identified as C. jejuni (89% = 42 strains). Standard tests failed to identify 9 strains; 6 strains were identified as Campylobacter jejuni and C. coli using the PCR method. Serotyping using 34 antisera of the Penner's serotyping scheme was performed in 130 strains of Campylobacter jejuni isolated from poultry and human patients. This method was suitable for identification of almost 80% of C. jejuni strains. We found 10 different serotypes of human C. jejuni strains. There were serotypes (HS) 4, 1 and 9 (32%, 23% and 15%, respectively) predominating in the human population; they were followed by serotypes (HS) 10, 2 and 23 (8%, 5% and 3%, respectively). Animal strains included 7 (HS) serotypes. Like in the human strains, the serotype 4, found in 37% of examined isolates, was predominating. Serotypes 23 and 1 (17% and 15%, respectively) were the next most frequent ones. Comparing the obtained serotypes of human and animal strains we see that there are only 4 identical ones (i.e., 4, 1, 10, 23), which are, however, most frequently occurring in both groups of strains.The PCR methods and serotyping may facilitate and make more accurate the results of standard cultivation and in epidemiologically serious cases help in explaining the route of transmission.

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“…In contrast, the predominant LG genotype of strains belonging to the HS-4 complex serogroup was only found in 38% of the strains tested. Penner serogroups 1, 2, 3, and especially 4, which together comprise a large proportion of clinical isolates in certain parts of the world, are not clonal but represent a genetically diverse population (2,13,14,19,27,32). In this respect, the strong correlation between LG genotype and HS-1, HS-2, and HS-3 serotype is remarkable.…”

Section: Discussionmentioning

confidence: 99%

Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

et al. 2002

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A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the lipopolysaccharide gene cluster. Sixty-one Penner serotype reference strains were analyzed with this new genotyping scheme, and 32 genogroups were found. Eleven additional genogroups were obtained from 87 clinical C. jejuni strains tested. This molecular typing method shows a correlation with the Penner heat-stable serotyping method, a phenotypic typing method based on lipopolysaccharide structures that is often used as a "gold standard" for subtyping Campylobacter spp. This strong correlation suggests that the data obtained can be directly compared with epidemiological data collected in the past by classical serotyping of C. jejuni and C. coli. In contrast to the high percentage of nontypeability by phenotyping, this molecular typing method results in 100% typeability and provides a superior alternative to serotyping.

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The serotype distribution of Campylobacter jejuni and C. coli isolated from patients with diarrhoea and controls at Tikur Anbassa Hospital, Addis Ababa, Ethiopia

Cited by 14 publications

References 27 publications

Serotyping Scheme for Campylobacter jejuni and Campylobacter coli Based on Direct Agglutination of Heat-Stable Antigens

Serotyping Scheme for Campylobacter jejuni and Campylobacter coli Based on Direct Agglutination of Heat-Stable Antigens

Serotyping and Identification of Campylobacter jejuni and Campylobacter coli Strains of Human and Animal Origin Using the PCR Method

Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

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