The serotype distribution of Streptococcus agalactiae (GBS) carriage isolates among pregnant women having risk factors for early-onset GBS disease: a comparative study with GBS causing invasive infections during the same period in Denmark

Slotved, Hans Christian; Frimodt‐Møller, Niels; Khalil, Mohammad; Nielsen, Stine Yde

doi:10.1186/s12879-021-06820-2

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…Maternal GBS colonization is a major determinant of EOGBS infections in neonates. European studies have shown similarity in serotype distribution between maternal colonized GBS and iGBS, and this nding indicates that EOGBS infection is caused by GBS transmission from mother to newborn [13,14].…”

Section: Discussionmentioning

confidence: 68%

Molecular characteristics and virulence factors of group B Streptococcus in South Korea according to the invasiveness

Choi,

Kim,

Kim

et al. 2024

Preprint

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Background: Group B Streptococcus (GBS) causes invasive infections in newborns and elderly individuals, but is a noninvasive commensal bacterium in most immunocompetent people. Recently, the incidence of invasive GBS infections has increased worldwide, and there is growing interest in the molecular genetic characteristics of invasive GBS strains. Vaccines against GBS are expected in the near future. Here, we aimed to analyze the molecular characteristics of GBS according to the invasiveness. Methods:We analyzed GBS isolates collected and stored in two hospitals in South Korea between January 2015 and December 2020. The invasiveness of these isolates was determined via a retrospective review of clinical episodes. Serotype and sequence type (ST) were determined using multiplex polymerase chain reaction (PCR) and multilocus sequence typing, respectively. Fourteen virulence factor-encoding genes of GBS were analyzed using multiplex PCR. Results: One hundred and twenty GBS isolates from 55 children and 65 adults were analyzed; among them, 41 (34.2%) were invasive infection-related GBS isolates (iGBS). The most frequently detected serotype was III (39/120, 32.5%), and it accounted for a high proportion of iGBS (21/41, 51.2%). The most frequent ST was ST19 (18/120, 15.0%), followed by ST2 (17/120, 14.2%). Serotype III/ST17 was predominant in iGBS (12/41, 29.3%), and all 17 ST2 strains were noninvasive. The distribution of most of the investigated virulence factors was not significantly related to invasiveness; noteworthily, most of the serotype III/ST17 iGBS carried pilus island (PI) 2b (10/12, 83.3%), and the prevalence of fbsB was significantly low compared with noninvasive GBS isolates (P = 0.004). Characteristically, the combination of bca(+)-cspA(+)-pavA(+)-fbsB(-)-rib(+)-bac(-) was predominant in iGBS (24.4%, 10/41). Conclusions: Serotype III/ST17 GBS carrying PI-2b was frequently detected in iGBS. There was no significant association between invasiveness and the pattern of virulence factors; however, a specific combination of virulence factors was predominant in iGBS.

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Section: Discussionmentioning

confidence: 68%

Molecular characteristics and virulence factors of group B Streptococcus in South Korea according to the invasiveness

Choi,

Kim,

Kim

et al. 2024

Preprint

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“…They usually involve real-time PCR, either "in house" assays, dedicated to a number of PCR devices or molecular platforms. All of them differ in sensitivity and specificity of the results [1,[31][32][33][34][35][36][37][38][39][40][41][42][43].…”

Section: Discussionmentioning

confidence: 99%

An Application of Real-Time PCR and CDC Protocol May Significantly Reduce the Incidence of Streptococcus agalactiae Infections among Neonates

et al. 2022

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Streptococcus agalactiae is an important human opportunistic pathogen, especially infectious for pregnant women and neonates. This pathogen belongs to beta hemolytic Streptococcus spp. representatives and accounts for a significant part of early infections in newborns, including serious life-threatening infections. This research investigated the usefulness of Centers for Disease Control and Prevention (CDC) protocol for S. agalactiae DNA detection in 250 samples of recto-vaginal swabs collected from pregnant women (at 35–37 weeks of gestation) and pre-cultured overnight in liquid medium. With an application of the CDC protocol-based real-time PCR, the cfb gene was detected in 68 (27.2%) samples compared to 41 (16.4%) for the standard culture-based methodology. The applied molecular method presented high sensitivity (100.0%) and specificity (87.1%). Therefore, it allowed for more precise detection of S. agalactiae bacteria, compared to the reference diagnostic method, culture on solid media with the following strain identification. The increased sensitivity of GBS detection may result in a reduced number of infections in newborns and leads to more targeted antimicrobial prophylaxis therapy of GBS infections in pregnant women. In addition, the use of the molecular method allows for a significant reduction in the time needed to obtain a result for GBS detection, and interpretation of the results is relatively simple. Therefore, it enables a faster intervention in case of a necessity of an antibiotic therapy introduction in pregnant women whose GBS status is unknown at the time of delivery.

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“…Bacteria were grown in Tryptone Soya Broth medium (TSB) (Oxoid CM0129) or on TSB agar plates (TSA) (Oxoid CM0131) at 37 °C with shaking (180 RPM). Identification and serotyping of the GBS isolates was performed as previously described [ 27 ]. The genomic sequence data for the 22 clinical isolates have been deposited at the European Nucleotide Archive (ENA) under Bioproject no.…”

Section: Methodsmentioning

confidence: 99%

Identification of antibiotic induced persister cells in Streptococcus agalactiae

Greve,

Slotved,

Olsen

et al. 2024

PLoS ONE

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Antibiotic persistence is a phenomenon, where a small fraction of a bacterial population expresses a phenotypic variation that allows them to survive antibiotic treatment, which is lethal to the rest of the population. These cells are called persisters cells, and their occurrence has been associated with recurrent disease. Streptococcus agalactiae is a human pathobiont, able to cause invasive infections, and recurrent infections have been reported to occur in both newborns and adults. In this study, we demonstrated that S. agalactiae NEM316 can form persister cells when exposed to antibiotics from different classes. The frequency of persister cell formation was dependent on bacterial growth phase and the class of antibiotics. The ability to form persister cells in response to penicillin was shown to be a general trait among different clinical S. agalactiae isolates, independent of sero- and sequence-type. Taken together, this study shows the existence of antibiotic tolerant S. agalactiae persister cells, which may explain why this bacterial species frequently persists after treatment of invasive infection and can be associated with recurrent disease.

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The serotype distribution of Streptococcus agalactiae (GBS) carriage isolates among pregnant women having risk factors for early-onset GBS disease: a comparative study with GBS causing invasive infections during the same period in Denmark

Cited by 7 publications

References 27 publications

Molecular characteristics and virulence factors of group B Streptococcus in South Korea according to the invasiveness

Molecular characteristics and virulence factors of group B Streptococcus in South Korea according to the invasiveness

An Application of Real-Time PCR and CDC Protocol May Significantly Reduce the Incidence of Streptococcus agalactiae Infections among Neonates

Identification of antibiotic induced persister cells in Streptococcus agalactiae

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