The Sesamum indicum Rhizosphere Associated Bacterium: A Source of Antifungal Compound

Chaudhary, Renu; Balhara, Meenakshi; Jangir, Deepak Kumar; Dangi, Mrridula; Dangi, Mehak; Khatri, Savita; Chhillar, Anil Kumar

doi:10.2174/1568026618666180206102140

Cited by 2 publications

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“…Considering that the leishmaniasis treatment with pentavalent antimonials is known to be ineffective and unsafe and therapies based on pentamidine and amphotericin B are considered toxic and exhibit recurrence rates, it is clear that new treatment alternatives are necessary [132][133][134][135][136]. Although widely described as a producer of antibacterial and antifungal compounds [41,42,[137][138][139], we emphasize that the Burkholderia species' antileishmanial activity was unknown so far. As we demonstrated in this study, Burkholderiales may offer promising candidates to treat neglected diseases of which resistance and toxicity of current treatments represent a global public health concern.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Cruz

Paula

Nogueira

et al. 2021

International Journal of Microbiology

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In this study, we report the isolation and identification of an endophytic strain of Burkholderia cepacia (COPS strain) associated with Polygala paniculata roots. Polygala plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. B. cepacia COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes aph(3′)-IIa and blaTEM-116, respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles gyrB, lepA, and phaC. The root endophyte B. cepacia COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 9341, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as Moniliophthora perniciosa, whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas Sclerotinia sclerotiorum and Ceratocystis paradoxa showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited S. epidermidis ATCC 35984, E. faecium ATCC 700221 (MIC values of 32 μg/mL for both), E. faecalis ATCC 29212 (64 μg/mL), and S. aureus ATCC 25923 (128 μg/mL). We observed moderate antagonistic activity against A. baumannii ATCC 19606 and E. coli ATCC 25922 (both at 512 μg/mL), as well as potent cytotoxic effects on Leishmania infantum and Leishmania major promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic B. cepacia strain associated with P. paniculata and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.

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Section: Discussionmentioning

confidence: 99%

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Cruz

Paula

Nogueira

et al. 2021

International Journal of Microbiology

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“…The strains of Burkholderia sp. RC1 and RC2 isolated from the sesame rhizosphere demonstrated good antifungal activity against A. fumigatus and A. niger (Chaudhary et al, 2018). Most of the related biological control studies are about plant tion of this bacteria to postharvest diseases of fruits.…”

Section: Introductionmentioning

confidence: 99%

Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay

Shi¹,

Du²

2023

Europ.J.Hortic.Sci.

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Botrytis cinerea (gray mold) infection can causepostharvest quality decline of various fruits, resulting in huge economic losses. The use of fungicides to maintain fruit quality will lead to potential environmental pollution, harming human health. successfully applied to control postharvest diseases of fruits. In the present study, bacterial strain B-1 with a high antifungal activity against Botrytis cinerea was isolated from the surface of apricots. Combining morphological and biochemical characterization with 16S rDNA and recA gene sequencing, this bacterial Burkholderia contaminans, which is a kind of B. cepacia complex (Bcc). In order to further reveal its antifungal mechanism, the bacterial cells were lysed, and a protein with antifungal activity was isolated by DEAE-52 ionexchange chromatography and sephadex G-100 gel a molecular weight of 17.5 kD was analyzed by mass spectrometry and retrieved from ncbiprot database, and was determined to be as superoxide dismutase.The bacterial protein could effectively inhibit mycelia growth of B. cinerea, Penicillium expansum, and Alternaria tenuissima in vitro. The protein exhibited a broad-spectrum antifungal activity against nine pathogenic fungi. Moreover, it could cinee mildew in grape and strawberry fruits. After six days of inoculation with antifungal crude protein, the inhibition rates of grape and strawberry fruits were 88.97% and 86.55%, respectively. In addition, it could affect the DNA level, reactive oxygen species and mitochondrial membrane potential in pathogenic fungi. This study demonstrated that the B. contaminans B-1 strain had a great potential to be used a biocontrol microorganism to protect against postharvest fruit fungal decay.

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The Sesamum indicum Rhizosphere Associated Bacterium: A Source of Antifungal Compound

Abstract: Results from this study recommend that plant rhizosphere remains a rich hotspot for delivering a novel antifungal compounds.

Cited by 2 publications

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Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay

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