The severity of acute kidney injury correlates with plasma venom levels in Bothrops atrox envenomings

Brasileiro-Martins, Lisele Maria; Nascimento, Thaís Pinto; Silva-Neto, Alexandre Vilhena; Martins, Felipe Terra; Cavalcante, Sofia Angiole; Martins, Rodrigo Barros; Marques, Hedylamar Oliveira; Colombini, Mônica; Martins, Marilaine; Sartim, Marco A.; Wen, Fan Hui; Ferreira, Luíz Carlos de Lima; Sachett, Jacqueline de Almeida Gonçalves; Moura-da-Silva, Ana Maria; Aquino, Priscila Ferreira de; Monteiro, Wuelton Marcelo

doi:10.1016/j.toxicon.2022.09.010

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…The use of specific antibodies in the development of diagnostic tools (ELISA and lateral flow strip assays) for envenoming by venomous animals can accurately determine the type of snakebite incident and classify the severity of the case based on the amount of circulating venom, thus avoiding unjustified administration and unnecessary antivenom [ 11 , 40 , 50 , 60 , 61 , 84 ]. Consequently, the specificity of the antibodies present in antivenoms is associated with the venoms of species selected for hyperimmunization, and variations in intra/interspecific toxins can result in reduced recognition of toxins from different venoms [ 85 , 86 , 87 ].…”

Section: Biomarkers For Envenomation By Venomous Animals: What To Loo...mentioning

confidence: 99%

Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics

Cavalcante

Almeida

Moraes

et al. 2023

Toxins

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Specific and sensitive tools for the diagnosis and monitoring of accidents by venomous animals are urgently needed. Several diagnostic and monitoring assays have been developed; however, they have not yet reached the clinic. This has resulted in late diagnoses, which represents one of the main causes of progression from mild to severe disease. Human blood is a protein-rich biological fluid that is routinely collected in hospital settings for diagnostic purposes, which can translate research progress from the laboratory to the clinic. Although it is a limited view, blood plasma proteins provide information about the clinical picture of envenomation. Proteome disturbances in response to envenomation by venomous animals have been identified, allowing mass spectrometry (MS)-based plasma proteomics to emerge as a tool in a range of clinical diagnostics and disease management that can be applied to cases of venomous animal envenomation. Here, we provide a review of the state of the art on routine laboratory diagnoses of envenomation by snakes, scorpions, bees, and spiders, as well as a review of the diagnostic methods and the challenges encountered. We present the state of the art on clinical proteomics as the standardization of procedures to be performed within and between research laboratories, favoring a more excellent peptide coverage of candidate proteins for biomarkers. Therefore, the selection of a sample type and method of preparation should be very specific and based on the discovery of biomarkers in specific approaches. However, the sample collection protocol (e.g., collection tube type) and the processing procedure of the sample (e.g., clotting temperature, time allowed for clotting, and anticoagulant used) are equally important to eliminate any bias.

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Section: Biomarkers For Envenomation By Venomous Animals: What To Loo...mentioning

confidence: 99%

Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics

Cavalcante

Almeida

Moraes

et al. 2023

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

Immersing in a Neglected Problem Inside the Amazon Jungle: Abstracts of the 1st International Symposium on Research in Venomous Animals, Manaus, Brazil

et al. 2023

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The severity of acute kidney injury correlates with plasma venom levels in Bothrops atrox envenomings

Cited by 2 publications

References 27 publications

Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics

Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics

Immersing in a Neglected Problem Inside the Amazon Jungle: Abstracts of the 1st International Symposium on Research in Venomous Animals, Manaus, Brazil

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