The sex-specific metabolic signature of C57BL/6NRj mice during aging

Abstract

Due to intact reactive oxygen species homeostasis and glucose metabolism, C57BL/6NRj mice are especially suitable to study cellular alterations in metabolism. We applied Nuclear Magnetic resonance spectroscopy to analyze five different tissues of this mouse strain during aging and included female and male mice aged 3, 6, 12, and 24 months. Metabolite signatures allowed separation between the age groups in all tissues, and we identified the most prominently changing metabolites in female and male tissues. A refined analysis of individual metabolite levels during aging revealed an early onset of age-related changes at 6 months, sex-specific differences in the liver, and a biphasic pattern for various metabolites in the brain, heart, liver, and lung. In contrast, a linear decrease of amino acids was apparent in muscle tissues. Based on these results, we assume that age-related metabolic alterations happen at a comparably early aging state and are potentially associated with a metabolic switch. Moreover, identified differences between female and male tissues stress the importance of distinguishing between sexes when studying age-related changes and developing new treatment approaches. Besides, metabolomic features seem to be highly dependent on the genetic background of mouse strains.

Reduced liver damage and fibrosis with combined SCD Probiotics and intermittent fasting in aged rat

“…This study found significant increases in LDH and albumin levels in IF, SCD Probiotic and combined supplementation groups compared to the control group. Age‐related muscle and bone mass decreases might contribute to lower LDH levels 43,44 . Additionally, changes in ALP levels, an enzyme associated with liver, kidney, and bone tissue, might affect aging.…”

Castration Model Illuminates Sex Differences in Healthy Aging: Insights from Metabolome and Transcriptome Analyses

“…Given the known correlation between ages 10 – 14 months in mice and 38 – 47 years in humans, where senescence-related changes become prominent 15 , we conducted aging-related behavioral analyses. These included Barnes maze (BM), open field (OF) and novel object recognition (NOR) tasks to evaluate the spatial learning and memory, locomotor activity, and recognition memory in 12 – 13 month-old casM, shamM and shamF mice.…”

“…Hair graying, a common aging marker in both mice and humans [12], was notably delayed in casM compared to shamM at 13 months, indicating a more youthful phenotype in the castrated group ( Figure 1C ). Given the known correlation between ages 10 – 14 months in mice and 38 – 47 years in humans, where senescence-related changes become prominent [13], we conducted aging-related behavioral analyses. These included Barnes maze (BM), open field (OF) and novel object recognition (NOR) tasks to evaluate the spatial learning and memory, locomotor activity, and recognition memory in 12 – 13 month- old casM, shamM and sham female (shamF) mice.…”

“…Hair graying, a common aging marker in both mice and humans [12], was notably delayed in casM compared to shamM at 13 months, indicating a more youthful phenotype in the castrated group (Figure 1C). Given the known correlation between ages 10 -14 months in mice and 38 -47 years in humans, where senescence-related changes become prominent [13],…”

“…Three groups of mice include casM, shamM and shamF at 12-13 months old (n= [6][7][8][9][10][11][12][13] were subjected to BM, OF and NOR tasks. A 60 cm high, 120 cm-diameter and 60 mm thick circular wheeled PVC slab with 25 cm-diameter holes was used for BM tests.…”