The Sézary Syndrome Lymphoid Cell: Abnormal Surface Properties and Mitogen Responsiveness

Braylan, Raul C.; Variakojis, Daina; Yachnin, Stanley

doi:10.1111/j.1365-2141.1975.tb00890.x

Cited by 39 publications

(13 citation statements)

References 31 publications

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“…This procedure was similar to one already described (13), except that heat-inactivated human serum (type AB), previously absorbed with sheep red blood cells, was used in place of fetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

Demonstration of the inhibitory effect of human alpha-fetoprotein on in vitro transformation of human lymphocytes.

Yachnin¹

1976

Proc. Natl. Acad. Sci. U.S.A.

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We have studied the effects of human alphafetoprotein (HAFP), isolated from the serum and ascitic fluid of a hepatoma-bearing patient, on the in vitro transformation of human peripheral blood lymphocytes by a variety of mitogenic stimuli. At a concentration of 2.5 mg/ml, HAFP inhibited the Iymphocyte response to phytohemagglutinin, concanavalin A, and rabbit anti-human thymocyte serum, but failed to inhibit the response to pokeweed mitogen. HAFP Until recently, little was known of the biological role of the alpha-fetoprotein (1), although it was regarded as an embryonic serum albumin (2, 3) and was shown to possess estrogen hormone-binding properties (4). Our report on the inhibition of human lymphocyte transformation in vitro by fetuin (5), a bovine alpha-fetoprotein, and the simultaneous observations by Tomasi and his coworkers that murine alpha-fetoprotein suppresses murine lymphocyte responses in vitro to both mitogenic and antigenic stimuli (6, 7), suggest an immunoregulatory role for alpha-fetoproteins during fetal development. This paper presents experimental data that demonstrate the inhibitory effects of human alpha-fetoprotein (HAFP) on lymphocyte responses in vitro to a variety of mitogenic stimuli which include the one-way mixed lymphocytes culture. The results suggest that this effect may be due primarily to an inhibition of thymus-derived lymphocytic (T-lymphocyte) function. MATERIALS AND METHODSThe techniques for isolating, culturing, and harvesting cultures of human lymphocytes were previously described (5,(8)(9)(10). A 1 ml culture system was employed throughout, and all experiments with cultures were performed in triplicate. The effect of preexposure with HAFP on the viability of human lymphocytes was determined by trypan blue exclusion (5), and their ability to undergo subsequent transformation when subjected to a mitogenic stimulus was assessed. Lymphocytes (25-35 X 106), in a total volume of 2.0 ml of RPMI medium and 12.5% of human type AB serum, were incubated for 18 hr in the presence of 2.2 mg/ml of HAFP or HSA, with conditions identical to those used for lymphocyte cultures. A portion (4 X 106) of the lymphocytes was removed from the cell suspensions, spun down, and used for the determination of viability by the trypan blue method. The remainder of the cell suspension was diluted with 5 ml of Amos A gelatin (9) buffer and centrifuged at 150 X g for 15 min. The cell buttons were washed once more with 7 ml of Amos buffer, resuspended in culture medium, and used for transformation studies.The ability of HAFP to inhibit E-rosette formation by Ficoll-Hypaque-isolated human lymphocytes was determined by means of the E-rosette formation procedure, carried out in the presence of 2.5 mg/ml of HAFP . This procedure was similar to one already described (13), except that heat-inactivated human serum (type AB), previously absorbed with sheep red blood cells, was used in place of fetal calf serum.In addition to the effect of HAFP in the mixed lymphocyte culture (MLC), the effect of HAFP on l...

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Section: Methodsmentioning

confidence: 99%

Demonstration of the inhibitory effect of human alpha-fetoprotein on in vitro transformation of human lymphocytes.

Yachnin¹

1976

Proc. Natl. Acad. Sci. U.S.A.

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“…Those findings imply that two noncontiguous epidermal plaques must arise by hematogenous dissemination of the neoplastic (6,26), and chromosome breaks deletions, duplications, and rearrangements are probably late sequelae of the aberrant mitotic process in CTCL. Furthermore, the morphologic abnormalities associated with CTCL are not specific for the disorder and have been noted in normal mitogen-activated lymphocytes, as well as in benign skin infiltrates (27).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of cutaneous T cell lymphoma by use of monoclonal antibodies reactive with tumor-associated antigens.

Berger¹,

Morrison²,

Chu³

et al. 1982

J. Clin. Invest.

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“…The Sézary syndrome is generally thought to be a T cell malignancy [3,4], al though there are a few reports on 'null-cell' characteristics of malignant cells in this dis ease [2,7] as well as on T and B cell ('chi merical) characteristics (5]. Our case shows an apparent change in immunological char acteristics from 'non-B/non-T cell' to T cell malignancy in terms of E rosette formation with a slight increase in PAS stainability and occurrence of tartrate-resistant acid phosphatase in the atypical cells.…”

Section: Discussionmentioning

confidence: 99%

Sézary Syndrome: Immunocytological and Cytochemical Variability of Sézary Cells

Konrads¹,

Laaser²,

Hummerich³

et al. 1980

Acta Haematol

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The characteristic large cells in the blood of a patient with Sézary syndrome underwent immunocytological and cytochemical changes during polychemotherapy, which caused transient regression of skin lesions. Tartrate-resistant acid phosphatase was demonstrable in a few cells only after chemotherapy; initially, only 2% T cells and 26% B cells could be demonstrated in the blood by immunocytological methods; after cytostatic therapy, 35% T cells but no B cells were detected.

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The Sézary Syndrome Lymphoid Cell: Abnormal Surface Properties and Mitogen Responsiveness

Cited by 39 publications

References 31 publications

Demonstration of the inhibitory effect of human alpha-fetoprotein on in vitro transformation of human lymphocytes.

Demonstration of the inhibitory effect of human alpha-fetoprotein on in vitro transformation of human lymphocytes.

Diagnosis of cutaneous T cell lymphoma by use of monoclonal antibodies reactive with tumor-associated antigens.

Sézary Syndrome: Immunocytological and Cytochemical Variability of Sézary Cells

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