The shape-effect of calcium phosphate nanoparticle based films on their osteogenic properties

Sutthavas, Pichaporn; Habibović, Pamela; Rijt, Sabine van

doi:10.1039/d0bm01494j

Cited by 13 publications

(6 citation statements)

References 88 publications

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“…The surfacegrafted amines could then be further modified by reaction with succinic anhydride to carboxylic acid groups (MSN-COOH), to allow Ca and P deposition on the surface of MSNs., as we reported previously. [15] The surface modification resulted in another surface potential change to −17.7 ± 0.2, indicating that the modification from positively charged amine groups to negatively charged carboxylic acid groups was successful (Table S1, Supporting Information). The carboxylic acid group modification was further confirmed by Fourier transform infrared spectroscopy with an observable peak of around 1627 cm −1 (COOH stretch, Figure S2c, Supporting Information).…”

Section: Synthesis and Characterization Of Sr-loaded And Ca P Zn-surf...mentioning

confidence: 99%

Calcium Phosphate‐Coated and Strontium‐Incorporated Mesoporous Silica Nanoparticles Can Effectively Induce Osteogenic Stem Cell Differentiation

Sutthavas

Birgani

Habibović

et al. 2021

Adv Healthcare Materials

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Ceramic (nano)materials are promising materials for bone regeneration applications. The addition of bioinorganics such as strontium (Sr) and zinc (Zn) is a popular approach to further improve their biological performance. However, control over ion delivery is important to prevent off-target effects. Mesoporous silica nanoparticles (MSNs) are popular nanomaterials that can be designed to incorporate and controllably deliver multiple ions to steer specific regenerative processes. In this work, MSNs loaded with Sr (MSN Sr ) and surface coated with a pH-sensitive calcium phosphate (MSN Sr -CaP) or calcium phosphate zinc layer (MSN Sr -CaZnP) are developed. The ability of the MSNs to promote osteogenesis in human mesenchymal stromal cells (hMSCs) under basic cell culture conditions is explored and compared to ion administration directly to the cell culture media. Here, it is shown that MSN-CaPs can effectively induce alkaline phosphatase (ALP) levels and osteogenic gene expression in the absence of other osteogenic stimulants, where an improved effect is observed for MSNs surface coated with multiple ions. Moreover, comparatively lower ion doses are needed when using MSNs as delivery vehicles compared to direct ion administration in the medium. In summary, the MSNs developed here represent promising vehicles to deliver (multiple) bioinorganics and promote hMSC osteogenesis in basic conditions.

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Section: Synthesis and Characterization Of Sr-loaded And Ca P Zn-surf...mentioning

confidence: 99%

Calcium Phosphate‐Coated and Strontium‐Incorporated Mesoporous Silica Nanoparticles Can Effectively Induce Osteogenic Stem Cell Differentiation

Sutthavas

Birgani

Habibović

et al. 2021

Adv Healthcare Materials

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“…To allow Ca, P and Zn ion deposition on the surface and within mesopores, the MSNs, DMSN and MBGZn were first aminated by postgrafting using APTES. The surface-grafted amines were subsequently modified to carboxylic acid groups by reaction with succinic anhydride, as we reported recently [ 25 ]. Zn ions were then incorporated into the mesopores of modified MSNs and DMSNs by physical diffusion to create Zn-loaded MSNs and Zn-loaded DMSNs (MSN Zn and DMSN Zn , respectively).…”

Section: Resultsmentioning

confidence: 99%

“…Under reflux conditions, 1 mL of APTES was introduced under vigorous stirring and left stirring for 24 h. The nanoparticles were collected via centrifugation and washed with absolute ethanol three times. The amine groups were modified to carboxylic acid groups using our previously reported method [ 25 ]. In short, 3 g of succinic anhydride were dissolved in 20 mL DMF and left stirring for 30 min at room temperature to allow the complete dissolution of succinic anhydride.…”

Section: Methodsmentioning

confidence: 99%

Zn-Loaded and Calcium Phosphate-Coated Degradable Silica Nanoparticles Can Effectively Promote Osteogenesis in Human Mesenchymal Stem Cells

et al. 2022

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Nanoparticles such as mesoporous bioactive glasses (MBGs) and mesoporous silica nanoparticles (MSN) are promising for use in bone regeneration applications due to their inherent bioactivity. Doping silica nanoparticles with bioinorganic ions could further enhance their biological performance. For example, zinc (Zn) is often used as an additive because it plays an important role in bone formation and development. Local delivery and dose control are important aspects of its therapeutic application. In this work, we investigated how Zn incorporation in MSN and MBG nanoparticles impacts their ability to promote human mesenchymal stem cell (hMSC) osteogenesis and mineralization in vitro. Zn ions were incorporated in three different ways; within the matrix, on the surface or in the mesopores. The nanoparticles were further coated with a calcium phosphate (CaP) layer to allow pH-responsive delivery of the ions. We demonstrate that the Zn incorporation amount and ion release profile affect the nanoparticle's ability to stimulate osteogenesis in hMSCs. Specifically, we show that the nanoparticles that contain rapid Zn release profiles and a degradable silica matrix were most effective in inducing hMSC differentiation. Moreover, cells cultured in the presence of nanoparticle-containing media resulted in the highest induction of alkaline phosphate (ALP) activity, followed by culturing hMSC on nanoparticles immobilized on the surface as films. Exposure to nanoparticle-conditioned media did not increase ALP activity in hMSCs. In summary, Zn incorporation mode and nanoparticle application play an important role in determining the bioactivity of ion-doped silica nanoparticles.

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“…Surface topography affects the pattern of gene expression of bone-related proteins (OSP, OSN, bone sialoprotein, type I collagen, and ALP) (Thalji and Cooper, 2013). ALP is an enzyme that acts as a mineralization promoter (Sutthavas et al, 2021). RUNX2 is a marker in the first stage of bone formation, in which mesenchymal precursors commit to osteoblast differentiation lineage (Yuan et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16

Hua

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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The hydroxyapatite (HA) coating on carbon/carbon (C/C) is reasonable and feasible to obtain bone graft materials with appropriate mechanical and biological properties. However, improvement of the physical and chemical properties of HA-C/C composites to promote bone regeneration and healing remains a challenge. In our present study, the HA coatings on C/C with magnesium (Mg) (Mg-HA-C/C) composites were synthesized that Ca (NO3)2, Mg (NO3)2, and NH4H2PO4 were mixed and coatings were made by electromagnetic induction deposition’s heating. As determined with in vitro experiments, Mg-HA-C/C composites containing 10 and 20% Mg decreased miR-16 levels, increased cell viability, elevated the levels of osteogenesis-related genes, and promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) seeded on their surfaces. In a rat model of skull defects, compared to the control group, at 4 and 12 weeks after the operation, the bone volume fraction (BV/TV) of Mg-HA-C/C composite group was increased by 8.439 ± 2.681% and 23.837 ± 7.845%, as well as the trabecular thickness (Tb.Th) was 56.247 ± 24.238 μm and 114.911 ± 34.015 μm more. These composites also increased the levels of ALP and RUNX2 in skull. The Mg-HA-C/C composite-enhanced bone regeneration and healing were blocked by in situ injection of an miR-16 mimic lentivirus vector. Thus, Mg-HA-C/C composites promote osteogenic differentiation and repair bone defects through inhibiting miR-16.

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The shape-effect of calcium phosphate nanoparticle based films on their osteogenic properties

Abstract: Calcium phosphate nanoparticle based films show shape dependent increase of hMSC osteogenic marker expression and hMSC morphology.

Cited by 13 publications

References 88 publications

Calcium Phosphate‐Coated and Strontium‐Incorporated Mesoporous Silica Nanoparticles Can Effectively Induce Osteogenic Stem Cell Differentiation

Calcium Phosphate‐Coated and Strontium‐Incorporated Mesoporous Silica Nanoparticles Can Effectively Induce Osteogenic Stem Cell Differentiation

Zn-Loaded and Calcium Phosphate-Coated Degradable Silica Nanoparticles Can Effectively Promote Osteogenesis in Human Mesenchymal Stem Cells

Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16

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